宫颈癌乏氧诱导因子-1α和谷胱甘肽-S-转酶-π的表达及与化疗药物耐药性关系的研究

发布时间：2018-06-15 17:40

本文选题：乏氧诱导因子-α + 谷胱甘肽-S-转移酶-π　；参考：《中国现代医学杂志》2015年13期

【摘要】：目的观察乏氧诱导因子-1α(HIF-1α)和谷胱甘肽-S-转酶-π(GST-π)在宫颈癌中的表达,探讨其与化疗药物敏感性的关系。方法应用MTT法对71例未经化疗的宫颈癌患者行体外化疗药物阿霉素、卡铂、紫杉醇5-氟尿嘧啶和顺铂的敏感性检测,并用免疫组织化学方法对HIF-1α和GST-π在宫颈癌中的表达进行检测,将其表达情况与对应的化疗耐药性进行相关分析。结果 71例宫颈癌患者对紫杉醇(TAXOL)、顺铂(DDP)、阿霉素(ADM)、5-氟尿嘧啶(5-Fu)和卡铂(CBP)的耐药率分别为38.03%(27例)、53.50%(38例)、54.93%(39例)、26.80%(19例)和47.90%(34例)。腺癌耐药例数多于鳞癌,但两者差异无统计学意义(P0.05)。HIF-1α和GST-π在71例宫颈癌患者中阳性率分别为56.34%(40/71)和57.75%(41/71),HIF-1α和GST-π在腺癌中的阳性率高于鳞癌,但两者差异无统计学意义(P0.05)。HIF-1α和GST-π在宫颈癌中的表达呈明显相关,P0.05。HIF-1α和GST-π的表达与化疗药物敏感性之间均存在明显相关(P0.05)。结论 HIF-1α和GST-π可能共同参与介导宫颈癌的多药耐药,两者在宫颈癌中的阳性表达及共表达可作为预测宫颈癌患者多药耐药的指标之一。
[Abstract]:Objective to investigate the expression of hypoxia inducible factor 1 伪 (HIF-1 伪) and glutathione -Stransferase 蟺 (GST- 蟺) in cervical cancer, and to explore the relationship between the expression of HIF-1 伪 and chemotherapeutic drug sensitivity. Methods the sensitivity of adriamycin, carboplatin, paclitaxel 5-fluorouracil and cisplatin was detected by MTT assay in 71 patients with cervical cancer without chemotherapy. The expression of HIF-1 伪 and GST- 蟺 in cervical cancer was detected by immunohistochemical method. The expression of HIF-1 伪 and GST- 蟺 was correlated with the corresponding chemoresistance. Results the drug resistance rates of 71 patients with cervical cancer to TAXOLG, DDP, adriamycin and adriamycin were 38.03 (27 cases) and 53.50% (38 cases), respectively (54.9339 cases (26.80%) and 47.90% (34 cases), respectively. The positive rates of P0.05 HIF-1 伪 and GST- 蟺 in 71 patients with cervical cancer were 56.34 and 40 / 71, respectively. The positive rates of HIF-1 伪 and GST- 蟺 in adenocarcinoma were higher than those in squamous carcinoma. However, there was no significant difference between the expression of P0.05A, HIF-1 伪 and GST- 蟺 in cervical cancer. There was a significant correlation between the expression of P0.05HIF-1 伪 and GST- 蟺 and the sensitivity of chemotherapeutic agents. Conclusion HIF-1 伪 and GST- 蟺 may be involved in the pathogenesis of multidrug resistance in cervical cancer. The positive expression and co-expression of HIF-1 伪 and GST- 蟺 may be used as predictors of multidrug resistance in cervical cancer.
【作者单位】：湖北省宜昌市五峰土家族自治县人民医院妇产科;湖北省人民医院妇产科;
【分类号】：R737.33

【参考文献】