IL-27及其受体在未足月胎膜早破发病中的相关研究

发布时间：2018-06-15 21:36

本文选题：未足月胎膜早破早产 + 胎膜组织　；参考：《重庆医科大学学报》2017年08期

【摘要】：目的:探讨白介素-27(interleukin-27,IL-27)及其特异性受体WSX-1/TCCR诱导人胎膜组织产生炎性效应在未足月胎膜早破中的作用。方法:(1)将手术室收集到的胎膜组织分为正常足月组和未足月胎膜早破组,将培养的胎膜组织分为对照组、实验组(IL-27处理12 h组以及IL-27处理24 h组);(2)Western blot和免疫组化分析WSX-1在未足月胎膜早破早产和正常足月孕妇胎膜组织中的表达;(3)培养原代人胎膜组织,鉴定其是否存活;(4)IL-27处理培养的胎膜组织,提取处理的人胎膜组织蛋白,用荧光定量PCR和明胶酶谱检测MMP2、MMP9的表达。结果:(1)IL-27特异性受体WSX-1在未足月胎膜早破早产孕妇胎膜组织中的表达明显高于正常足月孕妇(P=0.0149);(2)成功培养原代人胎膜组织;(3)人重组IL-27蛋白刺激胎膜组织后MMP2、MMP9的表达明显升高。结论:IL-27可诱导人胎膜组织产生炎性效应,增强MMP2和MMP9的表达,参与未足月胎膜早破早产的发病。
[Abstract]:Aim: to investigate the role of interleukin-27 (IL-27) and its specific receptor WSX-1 / TCCR in inducing inflammation in human fetal membranes. Methods the fetal membrane tissue collected in the operating room was divided into normal term group and non term premature rupture group, and the cultured fetal membrane tissue was divided into control group. Experimental group treated with IL-27 for 12 h and IL-27 treated group for 24 h Western blot and immunohistochemical analysis were used to analyze the expression of WSX-1 in premature rupture of unmatured membranes and normal fetal membranes. The human fetal membrane tissue was extracted and the expression of MMP2mMP9 was detected by fluorescence quantitative PCR and gelatin zymogram. Results the expression of IL-27 specific receptor WSX-1 in preterm premature rupture of unmatured membranes was significantly higher than that in normal pregnant women (P < 0.0149) the expression of MMP2MMP9 in human fetal membrane tissue was significantly increased after human recombinant IL-27 protein was stimulated by recombinant IL-27 protein. Conclusion: 1 / IL-27 can induce inflammatory effect in human fetal membranes, enhance the expression of MMP2 and MMP9, and participate in preterm premature rupture of preterm membranes.
【作者单位】：重庆医科大学附属第一医院妇产科;
【基金】：国家自然科学基金面上资助项目(编号:81471472)
【分类号】：R714.433

【相似文献】