CCCTC-结合因子（CTCF）及端粒酶hTERT在人子宫内膜癌组织中的表达研究

发布时间：2018-06-16 00:13

  本文选题：CCCTC-结合因子（CTCF） + 端粒酶基因hTERT蛋白　； 参考：《重庆医科大学》2014年硕士论文

【摘要】：第一部分应用Western-blotting技术检测人子宫内膜癌组织中CTCF和端粒酶基因hTERT蛋白表达的研究 目的：检测对比人子宫内膜癌和正常子宫内膜组织CTCF和端粒酶基因hTERT蛋白表达情况，结合临床病理参数分析子宫内膜癌组CTCF及hTERT分别与之关联，最后分析CTCF与hTERT之间联系，为临床奠定一定基础。 方法：随机选取30例正常人子宫内膜组织标本（其中15例增殖期内膜组织，15例分泌期内膜组织）为对照组，45例子宫内膜癌患者癌组织标本为实验组。采用Western-blotting法检测CTCF蛋白和端粒酶基因hTERT（Human telomerase reverse transcriptase,hTERT）蛋白在各组的表达水平。 结果：CTCF和hTERT在各组中均有表达。实验组CTCF蛋白和端粒酶基因hTERT蛋白阳性表达率均高于对照组。实验组与对照组间CTCF和端粒酶基因hTERT蛋白相对表达量均存在统计学差异。子宫内膜癌组CTCF表达与子宫内膜癌的病理分级、临床分期均有关，与病理类型无关；实验组hTERT表达与子宫内膜癌病理分级、病理类型、临床分期均无关。子宫内膜癌组CTCF与hTERT异常的表达存在正相关性。 结论：CTCF与端粒酶基因hTERT蛋白在人子宫内膜癌和正常子宫内膜中表达存在差异性；不同临床病理参数下CTCF和hTERT表达情况各异；在子宫内膜癌中两者联合检测对子宫内膜癌的诊断具有一定的意义。 第二部分应用半定量RT-PCR法检测人子宫内膜癌组织中CTCF和hTERTmRNA表达的研究 目的：检测对比人子宫内膜癌组织与正常子宫内膜组织中CTCFmRNA与hTERTmRNA的表达情况，分析其临床意义。 方法：从收集好的30例人正常子宫内膜标本（15例增殖期内膜组织，15例分泌期内膜组织）中挑选出WB实验CTCF表达阳性样本组织进行CTCFmRNA检测、hTERT表达阳性样本组织进行hTERTmRNA检测；从45例子宫内膜癌患者的癌组织标本中挑选出WB实验CTCF表达阳性样本组织进行CTCFmRNA检测、hTERT表达阳性样本组织进行hTERTmRNA检测；分别先后提取RNA,采用半定量RT-PCR法检测各标本中mRNA表达情况。 结果：子宫内膜癌组CTCFmRNA和端粒酶hTERTmRNA各自相对表达量与对照组间存在统计学差异。 结论：CTCFmRNA和端粒酶hTERTmRNA各自在实验组与对照组间存在不同，利用这一差异性可为临床诊断及治疗提供一定的基础。
[Abstract]:The first part used Western-blotting technique to detect the expression of CTCF and telomerase gene hTERT protein in human endometrial carcinoma. Objective: to detect and compare the expression of CTCF and telomerase gene hTERT protein in human endometrial carcinoma and normal endometrial tissue. The relationship between CTCF and hTERT in endometrial carcinoma was analyzed with clinicopathological parameters. Finally, the relationship between CTCF and hTERT was analyzed, which laid a foundation for clinical practice. Methods: 30 normal human endometrial tissues (15 proliferative endometrial tissues and 15 secretory endometrial tissues) were randomly selected as control group and 45 endometrial carcinoma specimens as experimental group. Western blotting was used to detect the expression of CTCF protein and human telomerase reverse transcriptasehTERT protein in each group. Results the expression of 1% CTCF and hTERT was found in all groups. The positive expression rate of CTCF protein and telomerase gene hTERT protein in experimental group was higher than that in control group. The relative expression of CTCF and telomerase gene hTERT was significantly different between the experimental group and the control group. The expression of CTCF in endometrial carcinoma group was related to the pathological grade and clinical stage of endometrial carcinoma, but not to pathological type, while hTERT expression in experimental group was not related to pathological grade, pathological type and clinical stage of endometrial carcinoma. There was a positive correlation between CTCF and hTERT expression in endometrial carcinoma. Conclusion there is a difference between the expression of human CTCF and telomerase gene hTERT in human endometrial carcinoma and normal endometrium, the expression of CTCF and hTERT is different under different clinicopathologic parameters. The combined detection of both in endometrial carcinoma has a certain significance in the diagnosis of endometrial carcinoma. The second part used semi-quantitative RT-PCR to detect the expression of CTCF mRNA and hTERT mRNA in human endometrial carcinoma. Objective: to detect and compare the expression of CTCF mRNA and hTERT mRNA in human endometrial carcinoma and normal endometrial tissue, and to analyze its clinical significance. Methods: a total of 15 cases of proliferative endometrial tissues were collected from 30 normal human endometrium specimens. The samples of CTCF positive expression were collected from 15 cases of secretory endometrial tissues in proliferative phase. The CTCF mRNA positive samples were detected for hTERT mRNA detection. From 45 samples of endometrial carcinoma, the samples of CTCF positive expression were collected from 45 patients with endometrial carcinoma. The positive samples of CTCF mRNA and hTERT expression were detected for hTERT mRNA. RNA was extracted successively and mRNA expression was detected by semi-quantitative RT-PCR. Results: the relative expressions of CTCF mRNA and telomerase hTERT mRNA in endometrial carcinoma group were significantly different from those in control group. Conclusion there are differences between the experimental group and the control group in the two groups. The use of this difference can provide a basis for clinical diagnosis and treatment.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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