胚胎期铅暴露对子代大鼠摄食和排便行为的影响及可能机制

发布时间：2018-06-16 11:51

本文选题：胃排空 + 小肠推进　；参考：《中国当代儿科杂志》2017年04期

【摘要】：目的探讨胚胎期铅暴露对子代大鼠摄食、排便行为的影响及可能机制。方法采用SpragueDawley大鼠孕期自由饮用0.1%(低剂量铅暴露组)和0.2%醋酸铅(高剂量铅暴露组)溶液的方法建立胚胎期铅暴露模型,并设空白对照组。将各组母鼠娩出的雄性仔鼠纳入研究,各组分别取10只仔鼠观察其在摄食、排便、胃排空、小肠推进及胃黏膜病理性炎性反应等方面的变化特点;各组分别取8只仔鼠,应用电镜技术、免疫组化等方法从空肠微绒毛及细胞连接超微结构变化,以及摄食中枢胆囊收缩素-8(CCK-8)、胃动素(MTL)的表达等方面揭示胚胎期铅暴露仔鼠胃肠动力异常的可能机制。结果与对照组比较,高、低剂量铅暴露组仔鼠每日摄食量下降,粪便含水量增加,粪粒质量减少,小肠推进速度增加(P0.05);高剂量铅暴露组仔鼠胃排空能力较对照组下降(P0.05)。与对照组比较,胚胎期铅暴露仔鼠胃黏膜出现明显的病理炎性改变(P0.05);空肠微绒毛减少、长度短缩及上皮间桥粒数目减少、致密斑间隙扩大(P0.05);摄食中枢MTL、CCK-8表达增加(P0.05);上述改变的程度与铅暴露剂量间有剂量-效应关系。结论胃肠结构损害程度,以及CCK和MTL在中枢的表达水平具有铅剂量依赖性,这可能是胚胎期铅暴露仔鼠出现异常的摄食、排便及消化功能改变的重要机制。
[Abstract]:Objective to investigate the effect and possible mechanism of embryonic lead exposure on feeding and defecating behavior of offspring rats. Methods Sprague-Dawley rats were exposed to 0.1% and 0.2% lead acetate (high dose lead exposure group) during pregnancy, and blank control group was set up. The male pups of each group were taken into the study. The changes of feeding, defecation, gastric emptying, intestinal propulsion and pathological inflammatory reaction of gastric mucosa were observed in 10 young rats in each group, and 8 young rats in each group were taken from each group, respectively, to observe the changes in feeding, defecation, gastric emptying, small intestine propulsion and pathological inflammatory reaction of gastric mucosa. The ultrastructural changes of jejunum microvilli and cell junctions and the expression of cholecystokinin (CCK-8) and motilin (MTL) in the feeding center were studied by electron microscopy and immunohistochemistry. Results compared with the control group, the daily food intake decreased, fecal water content increased, fecal grain mass decreased, and the intestinal propulsion rate increased in the low dose lead exposure group, and the gastric emptying capacity of the high dose lead exposure group was lower than that of the control group. Compared with the control group, there were obvious pathological inflammatory changes in gastric mucosa, decrease of jejunum microvilli, shortening of length and reduction of the number of interepithelial desmosomes. The density spot gap was enlarged (P0.05) and the expression of CCK-8 was increased in the feeding center. There was a dose-effect relationship between the above changes and the exposure dose of lead. Conclusion the degree of gastrointestinal structure damage and the level of CCK and MTL expression in the central nervous system are dose-dependent, which may be an important mechanism of abnormal feeding, defecation and digestive function changes in fetal lead exposed mice.
【作者单位】：西安交通大学第二附属医院儿科;陕西省人民医院妇产科;
【基金】：陕西省科技攻关项目(2016SF-263) 陕西省人民医院孵化基金(2010)
【分类号】：R714.2

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