P57、P53、Her-2及Ki-67在胎盘绒毛水肿性病变中的表达及其意义

发布时间：2018-06-18 04:33

本文选题：水泡状胎块 + 流产水肿绒毛　；参考：《重庆医学》2017年04期

【摘要】：目的研究P57、P53、Her-2及Ki-67在流产绒毛水肿(HA)、部分性葡萄胎(PHM)及完全性葡萄胎(CHM)中的表达,探讨其在水泡状胎块发生、发展中的作用,为葡萄胎的早期诊断及鉴别诊断提供理论依据。方法采用免疫组织化学(EnVision法)分别检测P57、P53、Her-2及Ki-67在30例正常绒毛(NP)、30例HA、30例PHM及30例CHM中的表达。结果 P57在NP组、HA组、PHM组中的阳性表达率分别为96.66%、96.66%、93.33%,显著高于在CHM组(3.33%)中的表达(P0.01);P53在PHM组(53.33%)中表达明显高于在NP组(16.66%)和HA组(20.00%)中的表达,而明显低于在CHM组(80.00%)中的表达,差异有统计学意义(P0.05);Her-2在CHM组中的表达为36.66%,明显高于在NP组(10.00)和HA组(13.33%)中的表达(P0.05),但是Her-2在PHM组(23.33%)与CHM组中的表达差异无统计学意义(P0.05);PHM组和CHM组的Ki-67增殖指数分别为(48.03±18.77)%和(87.79±10.50)%,均高于HA组[(33.43±17.17)%]及NP组[(34.46±16.15)%],P0.01,而CHM组的Ki-67显著高于PHM组(P0.01)。结论 P57缺失而P53及Ki-67高表达可诊断为CHM;P57阳性而P53及Ki-67增殖指数均缺失或弱表达可诊断为HA。其他情况下均需要进一步做分子基因分型检测;Her-2的表达对HA、PHM及CHM的鉴别意义不大。
[Abstract]:Objective to study the expression of P57 and Ki-67 in Ham, PHM (partial hydatidiform mole) and CHM (complete hydatidiform mole) in order to provide theoretical basis for early diagnosis and differential diagnosis of hydatidiform mole. Methods Immunohistochemical envision method was used to detect the expression of P57, P53, Her-2 and Ki-67 in 30 cases of normal chorionic villi, 30 cases of PHM and 30 cases of CHM. Results the positive expression rates of P57 in the PHM group of NP group were 96.66666 and 93.33, respectively, which were significantly higher than those in CHM group (3.33) and in PHM group (53.33) and in NP group (16.66) and HA group (20.00), respectively, and were significantly lower than those in CHM group (80.005%). The expression of Her-2 in CHM group was 36.66, which was significantly higher than that in NP group (10.00) and HA group (13.3335), but the expression of Her-2 in PHM group (23.33) and CHM group was not significantly different from that in CHM group (Ki-67 proliferation index was found in PHM group and CHM group, respectively. The ratio of Ki-67 in CHM group was significantly higher than that in HA group (33.43 卤17.17%) and NP group (34.46 卤16.15%), but Ki-67 in CHM group was significantly higher than that in PHM group. Conclusion the high expression of p53 and Ki-67 and the deletion of p53 and Ki-67 can be diagnosed as positive expression of CHMN P57, while both deletion or weak expression of p53 and Ki-67 can be diagnosed as HAA. In other cases, the expression of Her-2 is of little significance to the identification of HAH PHM and CHM.
【作者单位】：重庆市合川区人民医院病理科;重庆市第三人民医院肿瘤科;
【基金】：重庆市卫生和计划生育委员会医学科研计划项目(20143054)
【分类号】：R737.33

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