肽基脯氨酰顺反异构酶抑制剂胡桃醌对宫颈鳞癌SiHa细胞凋亡的作用

发布时间：2018-06-18 04:16

本文选题：胡桃醌 + 肽基脯氨酰顺反异构酶　；参考：《中国病理生理杂志》2015年03期

【摘要】：目的:探讨肽基脯氨酰顺反异构酶抑制剂胡桃醌对宫颈鳞癌Si Ha细胞增殖和凋亡的影响及其促凋亡的机制。方法:选取处于对数生长期的Si Ha细胞,将其分为空白对照组和不同剂量(10、20、50、80及100μmol/L)胡桃醌给药组,共6组。采用MTT法观察胡桃醌对Si Ha细胞的抑制作用,并计算出半数抑制浓度(IC50=20.4μmol/L),依据IC50确定胡桃醌的有效浓度;选取与IC50接近的浓度(20μmol/L)通过Hoechst 33258及流式细胞术测定胡桃醌对Si Ha细胞凋亡的影响,Western blot检测凋亡相关蛋白caspase-3、8、9以及抑癌基因PTEN的蛋白水平。结果:MTT实验结果显示,与对照组比较,各给药组对细胞活力的抑制作用明显,差异有统计学意义(P0.05);Hoechst 33258检测表明,20μmol/L胡桃醌处理细胞12 h后可出现明显的细胞核典型凋亡细胞形态,流式细胞术检测结果说明胡桃醌可诱导Si Ha细胞出现早期凋亡;Western blot检测显示与正常对照组相比,20μmol/L胡桃醌处理细胞12 h后,cleaved caspase-3、8、9及PTEN的蛋白水平明显增加。结论:胡桃醌可以抑制Si Ha细胞的活力,并诱导细胞凋亡,其机制主要是通过抑制caspase途径并增加抑癌基因的表达。
[Abstract]:Aim: to investigate the effects of peptidyl prolyl cis-trans isomerase inhibitor (Nutanone) on proliferation and apoptosis of cervical squamous cell carcinoma (SIHA) cells and the mechanism of promoting apoptosis. Methods: SIHA cells at logarithmic growth stage were divided into six groups: blank control group and different dosage of 10 ~ (20) ~ (20) ~ (50) and 100 渭 mol / L ~ (-1) walnut quinone group (n = 6). MTT assay was used to observe the inhibitory effect of walnut quinone on SIHA cells, and the half inhibitory concentration (IC50 渭 mol / L) was calculated. The effective concentration of Nutanone was determined according to IC50. The effect of nutquinone on apoptosis of SIHA cells was determined by Hoechst 33258 and flow cytometry. The protein levels of caspase-3, caspase-3 and PTEN, the tumor suppressor gene, were detected by Western blot. Results compared with the control group, the cell viability was inhibited obviously in the control group, and the difference was statistically significant (P 0.05) Hoechst 33258 showed that the typical apoptotic cell morphology appeared 12 h after treated with 20 渭 mol / L of pectin. The results of flow cytometry showed that walnut quinone could induce early apoptosis in SIHA cells. Western blot analysis showed that the protein levels of caspase-3, caspase-3 and PTEN were significantly increased after treated with 20 渭 mol / L pectin for 12 h compared with the normal control group. Conclusion: walnut quinone can inhibit the activity of SIHA cells and induce apoptosis by inhibiting the caspase pathway and increasing the expression of tumor suppressor genes.
【作者单位】：吉林医药学院;
【基金】：国家自然科学基金资助项目(No.21102055) 吉林省科技厅科技发展项目(No.20130101157JC) 吉林省教育厅“十二五”教育技术研究项目(No.2012-314;2014-371)
【分类号】：R737.33

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