孕期营养影响子代肥胖发生模型的建立研究

发布时间：2018-06-21 05:36

本文选题：产前营养生理学现象 + 肥胖症　；参考：《中国全科医学》2015年20期

【摘要】：目的建立一种能够研究营养性肥胖妊娠妇女对子代肥胖影响的孕鼠肥胖模型。方法选取SPF级SD成年大鼠,将成功受孕孕鼠40只采用随机数字表法分为对照组(妊娠期间喂养基础饲料),全孕期高能饮食组(妊娠期间喂养高能模型饲料),孕早期高能饮食组(妊娠后前10 d喂养高能模型饲料,之后喂养基础饲料)和高能饮食组(妊娠开始喂养基础饲料,后10 d喂养高能模型饲料),每组各10只。在子代鼠出生后第4、9周,停止喂食12 h后,称取子代鼠体质量,量取体长,计算Lee's指数;在子代鼠股动脉采集0.5 ml血液标本,采用放射免疫分析法检测血糖、血脂、血胰岛素等指标,并计算胰岛素敏感指数(ISI);取子代鼠肾周和睾周脂肪组织,计算脂体比。采用不同的标准判断各组致肥率:(1)以超过对照组体质量20%为标准进行判断;(2)以超过对照组脂肪垫重量2倍标准差为标准进行判断;(3)以超过对照组Lee's指数2倍标准差为标准进行判断。结果出生后第9周,4组体质量、体脂肪、脂体比和Lee's指数比较,差异均有统计学意义(P0.05);其中全孕期高能饮食组和孕早期高能饮食组体质量、体脂肪、脂体比和Lee's指数均高于对照组(P0.05)。以标准(1)进行判断,孕早期高能饮食组在子代鼠出生后第4周和第9周致肥率均为60%;全孕期高能饮食组在子代鼠出生后第4周和第9周致肥率均为70%;以标准(2)进行判断,孕早期高能饮食组在子代鼠出生后第4周和第9周致肥率均为70%;全孕期高能饮食组在子代鼠出生后第4周和第9周致肥率均为80%;以标准(3)进行判断,孕早期高能饮食组在子代鼠出生后第4周和第9周致肥率均为80%;全孕期高能饮食组在子代鼠出生后第4周和第9周致肥率均为90%。出生后第4周,各组血糖、血脂比较,差异无统计学意义(P0.05);各组血胰岛素、ISI比较,差异均有统计学意义(P0.05);其中全孕期高能饮食组和孕早期高能饮食组血胰岛素和ISI与对照组比较,差异均有统计学意义(P0.05)。出生后第9周,各组血胰岛素、ISI和血脂比较,差异均有统计学意义(P0.05),其中全孕期高能饮食组和孕早期高能饮食组血胰岛素、ISI和血脂与对照组比较,差异均有统计学意义(P0.05)。出生后第9周,各组肝脏、脂肪组织重量比较,差异均有统计学意义(P0.05);其中全孕期高能饮食组和孕早期高能饮食组肝脏和脂肪组织重量较对照组增加(P0.05)。结论通过调整孕鼠饮食数量及时间,出生后第4周可诱导建立营养性肥胖孕鼠对子代鼠肥胖影响模型,出生后第9周可建立营养性孕鼠对子代鼠肥胖和代谢综合征影响模型。
[Abstract]:Objective to establish a pregnant rat obesity model which can study the effect of nutritional obese pregnant women on offspring obesity. Methods SPF adult SD rats were selected. Forty successful pregnant rats were randomly divided into three groups: control group (fed with basic diet during pregnancy), high energy diet group during pregnancy (high energy diet during pregnancy) and high energy diet group in early pregnancy (first 10 after pregnancy). D feeding high energy model feed, Then the basic diet and the high energy diet group were fed with the basic diet at the beginning of pregnancy and the high energy model diet with 10 rats in each group 10 days after the pregnancy. At the 4th week after birth, after 12 hours of feeding, the offspring were weighed and measured to calculate the Leehs index, and 0.5 ml blood samples were collected from the femoral artery of the offspring, and blood glucose and blood lipid were detected by radioimmunoassay (RIA). Serum insulin, insulin sensitivity index (ISI) and adipose tissue around kidney and testis were calculated and the ratio of lipid to body was calculated. Using different criteria to judge the fatness rate of each group: 1) using 20% of the weight of the control group as the criterion) using the standard deviation of more than 2 times the weight of the fat pad in the control group as the standard to judge the weight of the fat pad in the control group as the standard 3) and exceeding the standard of Lees index of the control group by 2 times. The difference is judged by standard. Results there were significant differences in body mass, body fat, body fat ratio and Leehs index between the 4 groups at the 9th week after birth (P 0.05), in which the body weight and body fat of high energy diet group and early pregnancy high energy diet group were all significantly higher than that of the control group (P < 0. 05). The ratio of lipid to body and Leehs index were higher than that of control group (P 0.05). According to the standard (1), the fertilization rates in the first trimester group were 60 at the 4th and 9th weeks after birth; in the whole pregnancy high energy diet group were 70 at the 4th and 9th weeks after the birth of the offspring; In the first trimester group, the fertilization rates were both 70 in the 4th and 9th weeks after birth in the offspring, 80 in the 4th and 9th week after birth in the whole pregnancy group. In the first trimester group, the fertilization rate was 80% at the 4th and 9th week after birth, and 90% at the 4th week and the 9th week after birth in the whole pregnancy high energy diet group. At the 4th week after birth, there was no significant difference in blood glucose and blood lipids in each group (P 0.05). There were significant differences in serum insulin and ISI between the whole pregnancy high energy diet group and the early pregnancy high energy diet group compared with the control group. At the 9th week after birth, there were significant differences in serum insulin ISI and serum lipids between the two groups (P 0.05). There were significant differences in serum insulin ISI and blood lipids between the whole pregnancy high energy diet group and the early pregnancy high energy diet group compared with the control group (P 0.05). At the 9th week after birth, the weight of liver and adipose tissue in each group was significantly higher than that in control group, and the weight of liver and adipose tissue in high energy diet group and early pregnancy high energy diet group was higher than that in control group. Conclusion by adjusting the amount and time of diet of pregnant rats, the effects of nutritional obesity on offspring obesity can be established at the 4th week after birth, and the effects of nutritional pregnant mice on obesity and metabolic syndrome in offspring can be established at 9 weeks after birth.
【作者单位】：浙江省杭州市第一人民医院(南京医科大学杭州医院)儿科;
【基金】：杭州市医药卫生科技计划项目(2012A003)
【分类号】：R714;R-332

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