上皮性卵巢组织G3BP和OPN表达及相关性研究

发布时间：2018-06-22 18:19

本文选题：卵巢癌 + GTP酶激活蛋白SH功能区结合蛋白　；参考：《中华肿瘤防治杂志》2016年21期

【摘要】：目的卵巢癌是妇科肿瘤中死亡率居首的常见恶性肿瘤,寻找新的生物学标志物以提高卵巢癌诊治的敏感性与特异性至关重要。本研究探讨GTP酶激活蛋白SH3功能区结合蛋白(Ras-GTPase-activating protein SH3domain binding protein,G3BP)和骨桥蛋白(osteopontin,OPN)在上皮性卵巢组织中的表达、意义及其表达的相关性,为卵巢肿瘤的临床诊断及治疗提供依据。方法采用免疫组织化学方法检测G3BP和OPN在来源于中国医科大学盛京医院2009-10-01-2015-05-31的11例正常卵巢组织、20例卵巢上皮性良性肿瘤组织、31例卵巢上皮性交界性肿瘤组织及60例卵巢上皮性恶性肿瘤组织中的表达情况;并对二者表达的相关性进行统计分析。结果 G3BP在正常卵巢上皮组织、卵巢上皮性良性肿瘤组织、卵巢上皮性交界性肿瘤组织及卵巢上皮性恶性肿瘤组织中的表达呈递增趋势,其中上皮性良性肿瘤组织与正常卵巢上皮组织比较,χ~2=66.38,P0.01;上皮性交界性肿瘤组织与上皮性良性肿瘤组织比较,χ~2=41.183,P0.01;上皮性恶性肿瘤组织与上皮性交界性肿瘤组织比较,χ~2=73.342,P0.01。OPN在正常卵巢上皮组织、卵巢上皮性良性肿瘤组织、卵巢上皮性交界性肿瘤组织及卵巢上皮性恶性肿瘤组织中的表达呈递增趋势,其中上皮性良性肿瘤组织与正常卵巢上皮组织比较,χ~2=60.005,P0.01;上皮性交界性肿瘤组织与上皮性良性肿瘤组织比较,χ~2=43.844,P0.01;上皮性恶性肿瘤组织与上皮性交界性肿瘤组织比较,χ~2=73.429,P0.01。G3BP与OPN在卵巢上皮性恶性肿瘤组织中的表达具有正相关性,r=0.351,P0.01。结论 G3BP和OPN表达升高与上皮性卵巢癌的发生发展有密切关系,且二者可能存在协同作用。
[Abstract]:Objective Ovarian cancer is the most common malignant tumor in gynecological tumors. It is very important to search for new biomarkers to improve the sensitivity and specificity of diagnosis and treatment of ovarian cancer. The purpose of this study was to investigate the expression of GTPase-activating protein SH3domain binding protein G3BP and osteopontin binding protein in epithelial ovarian tissues, and to provide evidence for clinical diagnosis and treatment of ovarian tumors. Methods Immunohistochemical method was used to detect G3BP and OPN in 11 normal ovarian tissues from Shengjing Hospital of China Medical University 2009-10-01-2015-05-31. 20 cases of ovarian epithelial benign tumor tissues and 31 cases of ovarian epithelial sexual intercourse borderline tumor tissues and 60 cases of ovarian epithelial sexual intercourse tissues were detected. Cases of ovarian epithelial malignant tumors expressed in the tissues; The correlation between them was analyzed statistically. Results the expression of G3BP in normal ovarian epithelium, ovarian epithelial benign tumor, ovarian epithelial sexual intercourse borderline tumor and ovarian epithelial malignant tumor showed an increasing tendency. The comparison between epithelial benign tumor and normal ovarian epithelial neoplasm was 66.38 (P0.01); that of epithelial sexual intercourse between epithelial benign tumor and epithelial benign tumor was 41.183 (P0.01); and that of epithelial malignant tumor was significantly higher than that of epithelial sexual intercourse (蠂 ~ 23.342P0.01.OPN). Normal ovarian epithelium, The expression of ovarian epithelial benign tumor, borderline ovarian epithelial tumor and ovarian epithelial malignant tumor showed an increasing trend. The comparison between epithelial benign tumor tissue and normal ovarian epithelial tissue, 蠂 ~ (2 +) ~ (22) (60.005) P _ (0.01), epithelial sexual intercourse boundary tumor tissue and epithelial benign tumor tissue, 蠂 ~ (2 +) 43.844 (P ~ (0.01), and epithelial malignant tumor tissue and epithelial sexual intercourse boundary tumor tissue, 蠂 ~ (2 +) ~ (22) 429 (P 0.01.G3BP), compared with epithelial sexual intercourse tissue (P _ (0.01), compared with epithelial benign tumor tissue and epithelial benign tumor tissue (P _ (0.01). The expression of OPN in epithelial ovarian neoplasms was positively correlated with that of 0.351P0.01. Conclusion the increased expression of G3BP and OPN is closely related to the occurrence and development of epithelial ovarian cancer, and there may be a synergistic effect between the expression of G3BP and OPN.
【作者单位】：鞍山市双山医院妇产科;中国医科大学盛京医院妇产科;
【基金】：国家青年科学基金(81001163) 辽宁省自然课题基金(2015020462) 沈阳市科技厅基金(F13-220-9-46)
【分类号】：R737.31

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