免疫组化分析FKBP51表达量在子宫内膜增殖期与分泌期的差异

发布时间：2018-06-22 20:39

本文选题：子宫内膜容受性 + FKBP51　；参考：《山东大学》2014年硕士论文

【摘要】：[目的]容受性较好的子宫内膜和发育正常的胚泡,是保证妊娠过程顺利进行的两个决定性因素。排卵正常的不孕患者最常见的妊娠失败原因就是子宫内膜容受性的降低导致的植入失败。在育龄女性多种疾病的发展进程中,都存在着各种各样导致子宫内膜容受性降低的因素,这些因素相互影响、相互作用最终导致了这些患者妊娠率的低下。弄清此过程中降低子宫内膜容受性的因素的作用机制,对指导临床采取干预措施提高患者的生育能力,增加妊娠率有着至关重要的意义。目前,大量研究关注于亲免素蛋白FKBP51,该蛋白在性激素的调节过程的许多方面都起到一定的作用,其表达受到多种性激素水平的调控,并且还作为性激素受体复合物的组成部分,调节性激素在各组织的作用水平。本研究旨在探索：伴随子宫内膜增殖期向分泌期的转换,其性激素水平变化是否会导致FKBP51蛋白在子宫内膜的表达水平产生明显的差异。 [方法]收集80例子宫内膜活检的内膜标本,制作子宫内膜组织组合芯片。对芯片上每一点标本进行病理分期,用免疫组化的方法检测FKBP51抗体在子宫内膜组织组合芯片(UTN801)上的表达。显微镜观察半定量评定FKBP51表达水平,使用灰度分析软件统计灰度值,采用SPSS v19.0中两独立样本T检验对阳性积分和灰度值进行统计学分析。 [结果]随性激素调节水平的变化,FKBP51在子宫内膜组织的表达水平存在显著差异,子宫内膜分泌期的FKBP51表达水平明显高于增殖期表达水平(P0.001)。 [结论]在子宫内膜组织,伴随性激素水平的变化,FKBP51的表达水平有显著的差异。正常子宫内膜周期中,增殖期向分泌期的转变,伴随着雌激素和雄激素水平作用的下调,孕激素作用水平的上调,在此过程中,FKBP51的表达水平随之升高,分泌期其表达水平显著高于增殖期。
[Abstract]:Objective: endometrium with good receptivity and blastocyst with normal development are two decisive factors to ensure the smooth progress of pregnancy. The most common cause of pregnancy failure in infertile patients with normal ovulation is the failure of implantation due to reduced endometrial receptivity. In the development process of many diseases of women of childbearing age, there are various factors that lead to the decrease of endometrial receptivity. These factors affect each other and ultimately lead to the low pregnancy rate of these patients. Understanding the mechanism of reducing endometrial receptivity in this process is of great significance in guiding clinical intervention to improve fertility and increase pregnancy rate. At present, a large number of studies have focused on the protein FKBP51, which plays a role in many aspects of the regulation of sex hormones, and its expression is regulated by various levels of sex hormones, and is also a component of the sex hormone receptor complex. To regulate the action of sex hormones in various tissues. The aim of this study was to explore whether the changes of sex hormone levels in endometrium may lead to significant differences in the expression of FKBP51 protein in endometrium with the transition from endometrial proliferation to secretory phase. Methods 80 endometrial specimens from endometrial biopsy were collected and microarray of endometrial tissue was made. The expression of FKBP51 antibody on UTN801 was detected by immunohistochemistry. The expression level of FKBP51 was evaluated by microscope. The gray value of FKBP51 was calculated by gray analysis software, and the positive score and gray value were analyzed statistically by T test of two independent samples in SPSS v19.0. [results] there was significant difference in the expression of FKBP51 in endometrial tissues. The expression level of FKBP51 in secretory phase of endometrium was significantly higher than that in proliferative phase (P0.001). [conclusion] there is significant difference in the expression of FKBP51 in endometrium with the change of sex hormone level. During the normal endometrial cycle, the transition from proliferative phase to secretory phase, with the down-regulation of estrogen and androgen levels, and the up-regulation of progesterone action, the expression of FKBP51 increased during the normal endometrial cycle. The expression level in secretory phase was significantly higher than that in proliferative stage.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.6

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