速激肽human Hemokinin-1类似物对人精子活力的影响

发布时间：2018-06-26 09:15

本文选题：人类速激肽-1 + 速激肽类似物　；参考：《兰州大学》2017年硕士论文

【摘要】：目的:本文旨在通过研究速激肽human hemokinin-1碳端类似物hHK-1(4-11)和氮端类似物hHK-1(1-7)、hHK-1(1-8)、hHK-1(1-9)、hHK-1(1-10)对精子活力的影响,通过这种方法找出速激肽作用于精子活力的最有效片段,通过这样的研究希望能为提高精子活力的药物提供新的思路,为临床的体外受精-胚胎移植以及卵胞浆内单精子注射等辅助生殖治疗手段中保持精子活力的药物提供新的线索。方法:通过化学合成法——多肽固相合成方法合成并验证了5个不同的人速激肽的片段,它们分别是人速激肽的碳端片段hHK-1(4-11),以及人速激肽的氮端类似物hHK-1(1-7)、hHK-1(1-8)、hHK-1(1-9)和hHK-1(1-10)。首先,我们分别检测了hHK-1(4-11)、hHK-1(1-7)、hHK-1(1-8)、hHK-1(1-9)和hHK-1(1-10)对正常精液PR级和PR+NP级精子活力影响,其次,我们通过流式细胞仪分析了正常成熟精子与上述药物共培养3小时和6小时后精子DNA碎片的产生情况。结果:不同浓度(1×10-3mol/L、1×10-4mol/L、1×10-5mol/L)的hHK-1(4-11)、hHK-1(1-7)、hHK-1(1-8)、hHK-1(1-9)和hHK-1(1-10)分别作用于精子,hHK-1(1-7)作用于正常精子90min后的PR和PR+NP级精子百分率和空白对照}D比起初始的PR和PR+NP级精子百分率,精子百分率的降低具有统计学意义(P0.05),但是hHK-1(4-11)、hHK-1(1-8)、hHK-1(1-9)和hHK-1(1-10)作用于正常精子90min的PR和PR+NP级精子百分率的降低无统计学差异。结论:hHK-1(4-11)、hHK-1(1-8)、hHK-1(1-9)和hHK-1(1-10)具有保持精子活力的作用,而hHK-1(1-7)并不具备保持精子活力的作用。另一方面,通过精子DNA碎片的分析结果显示:所有的速激肽类似物均没有使精子DNA碎片率上升,这就说明,这些药物并不存在精子毒性作用,其本身并不会对精子活力及完整性产生影响。
[Abstract]:Aim: to study the effects of fast kinin human hemokinin-1 carbon terminal analogue hHK-1 (4-11) and nitrogen terminal analogue hHK-1 (1-7) hHK-1 (1-8) hHK-1 (1-9) hHK-1 (1-10) on sperm motility, and to find out the most effective fragment of rapid kallikrein acting on sperm motility by this method. It is hoped that this study can provide new ideas for improving sperm motility and provide new clues for clinical in vitro fertilization-embryo transfer and assisted reproductive therapy such as intracytoplasmic sperm injection. Methods: five different fragments of human tachykinin, hHK-1 (4-11), hHK-1 (1-7) hHK-1 (1-8) hHK-1 (1-9) and hHK-1 (1-10), were synthesized and verified by the method of chemical synthesis and solid phase synthesis of peptides, which are the carbon terminal fragments of human tachykinin (hHK-1 (4-11) and the nitrogen terminal analogues of human tachykinin (hHK-1 (1-7) hHK-1 (1-8) hHK-1 (1-9) and hHK-1 (1-10). Firstly, we examined the effects of hHK-1 (4-11) hHK-1 (1-7) hHK-1 (1-8) hHK-1 (1-9) and hHK-1 (1-10) on the motility of normal semen PR and PR NP, respectively. The DNA fragments of normal mature spermatozoa co-cultured with these drugs for 3 and 6 hours were analyzed by flow cytometry. Results: hHK-1 (4-11) hHK-1 (1-7) hHK-1 (1-8) hHK-1 (1-9) and hHK-1 (1-10) treated with hHK-1 (1-7) and hHK-1 (1-10) at different concentrations (1 脳 10 ~ (-3) mol / L ~ (1) 脳 10 ~ (-4) mol / L ~ (-1) 脳 10 ~ (-5) mol / L) acted on the percentage of PR and PR NP grade sperm after normal sperm 90min, respectively. The decrease of sperm percentage was statistically significant (P0.05), but there was no significant difference between hHK-1 (4-11) hHK-1 (1-8) hHK-1 (1-9) and hHK-1 (1-10) on PR and PR NP level sperm percentage of normal sperm 90min. Conclusion 1: hHK-1 (4-11) hHK-1 (1-8) hHK-1 (1-9) and hHK-1 (1-10) can maintain sperm motility, but hHK-1 (1-7) does not. On the other hand, analysis of sperm DNA fragments showed that none of the tachykinin analogues increased the rate of sperm DNA fragments, suggesting that these drugs had no sperm toxicity. It does not in itself affect sperm motility and integrity.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.8

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