EPAS1、VEGF在子宫内膜癌中的表达及其临床意义

发布时间：2018-06-27 21:24

本文选题：缺诱导因子2 + 血管内皮生长因子　；参考：《苏州大学》2014年硕士论文

【摘要】：第一部分EPAS1、VEGF在子宫内膜癌中的表达情况目的研究人子宫内膜癌及正常子宫内膜组织中缺氧诱导因子2(EPAS1/HIF-2α)、血管内皮生长因子(VEGF) mRNA及蛋白的表达及差异情况。方法应用逆转录聚合酶链反应(RT-PCR)、免疫印迹(western blotting)法检测46例子宫内膜癌及正常子宫内膜组织中EPAS1、VEGF mRNA和蛋白的表达，分析其间表达的差异。结果EPAS1、VEGF在子宫内膜癌中的表达明显高于正常子宫内膜组织, EPAS1mRNA和蛋白水平均增高(t=4.5871,p=0.0048;t=19.4877,p=0.0001)，VEGF在mRNA和蛋白水平均增高(t=7.0495,p=0.0001;t=98.4145,p=0.0001)。结论子宫内膜癌组织中EPAS1和VEGF呈过量表达,且EPAS1的表达与VEGF呈显著正相关。第二部分EPAS1、VEGF在子宫内膜癌中表达的临床意义目的研究子宫内膜癌组织中EPAS1、VEGF和微血管密度（MVD）的表达与临床病理特征之间的关系。方法应用免疫组化SP法检测46例子宫内膜癌及正常子宫内膜组织中EPAS1、VEGF蛋白的表达和分布,同时检测MVD作为判断血管生成的指标，分析其间的相关性以及与肿瘤临床病理特征之间的关系。结果EPAS1、VEGF和MVD在子宫内膜癌组织中的表达明显高于正常子宫内膜组织,三者之间的表达具有显著相关性(EPAS1和VEGF之间, r=0.7358,P=0.0041;VEGF和MVD之间,r=0.8871,P=0.0001; EPAS1和MVD间, r=0.7360,P=0.0003),EPAS1和VEGF的阳性表达与子宫内膜癌的FIGO分期、浸润深度有关。结论子宫内膜癌组织中EPAS1和VEGF呈过量表达,且EPAS1的表达与VEGF及MVD呈显著正相关。EPAS1可通过上调VEGF表达来促进子宫内膜癌血管生成从而在子宫内膜癌的发生发展中起重要作用。
[Abstract]:Part one: expression of EPAS1 + VEGF in endometrial carcinoma objective to study the expression of hypoxia inducible factor 2 (EPAS1 / HIF-2 伪) and vascular endothelial growth factor (VEGF) mRNA and protein in human endometrial carcinoma and normal endometrial tissues. Methods reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot (western blotting) were used to detect the expression of EPAS1 (western blotting) mRNA and protein in 46 cases of endometrial carcinoma and normal endometrium. Results the expression of VEGF in endometrial carcinoma was significantly higher than that in normal endometrial tissue. The mRNA and protein levels of EPAS1 in endometrial carcinoma were significantly higher than those in normal endometrial tissue (t = 4.5871). The expression of VEGF in endometrial carcinoma was significantly higher than that in normal endometrial tissue (t = 4.5871). The mRNA and protein levels of VEGF in endometrial carcinoma were both increased (t = 7.0495p0.0001p0.0001t98.4145p0.0001). Conclusion the expression of EPAS1 and VEGF in endometrial carcinoma is overexpression, and the expression of EPAS1 is positively correlated with VEGF. The clinical significance of the expression of EPAS1 / VEGF in endometrial carcinoma objective to study the relationship between the expression of EPAS1 / VEGF and microvessel density (MVD) and clinicopathological features in endometrial carcinoma. Methods Immunohistochemical SP method was used to detect the expression and distribution of EPAS1 + VEGF protein in 46 cases of endometrial carcinoma and normal endometrium, and MVD was used as an index to judge angiogenesis. The relationship between the correlation and clinicopathological features of tumor was analyzed. Results the expression of VEGF and MVD in endometrial carcinoma was significantly higher than that in normal endometrial tissue. There was a significant correlation between the expression of EPAS1 and VEGF, the positive expression of EPAS1 and VEGF, and the positive expression of EPAS1 and VEGF in endometrial carcinoma were correlated with the Figo stage and the depth of invasion of endometrial carcinoma, and the positive expression of EPAS1 and VEGF was related to the stage and depth of invasion of endometrial carcinoma, and the positive expression of EPAS1 and VEGF was related to the stage and depth of invasion of endometrial carcinoma. Conclusion the expression of EPAS1 and VEGF in endometrial carcinoma is overexpression, and the expression of EPAS1 is positively correlated with VEGF and MVD. EPAS1 can promote angiogenesis of endometrial carcinoma by up-regulating VEGF expression, which may play an important role in the occurrence and development of endometrial carcinoma.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

【参考文献】