GRIM-19蛋白在taurolidine诱导宫颈癌细胞凋亡中的作用

发布时间：2018-06-27 22:59

本文选题：宫颈肿瘤 + 基因表达　；参考：《中国老年学杂志》2015年02期

【摘要】：目的探讨GRIM-19蛋白在taurolidine诱导人宫颈癌Hela细胞凋亡中的作用。方法应用四甲基偶氮唑蓝(MTT)法检测不同剂量组宫颈癌细胞(Hela细胞系)存活率的影响;采用流式细胞术检测不同剂量组Hela细胞的凋亡数据;采用Western印迹法检测各实验组中Hela细胞中GRIM-19蛋白的表达变化;同时采用酶联免疫吸附(ELISA)法检测半胱氨酸蛋白酶(caspase)9的活性。结果 MTT结果显示,taurolidine对Hela细胞生长有显著的抑制作用,流式细胞术结果显示出实验组Hela细胞凋亡比例显著上升,并呈现出明显的时程和量效关系(P0.05)。Western印迹分析显示,与对照组相比,GRIM-19蛋白在各实验组Hela细胞中的表达显著提高(P0.05),同样显示出了一定的时程和量效关系。ELISA检测结果显示,caspase-9在各实验组Hela细胞中的表达显著增加,与GRIM-19蛋白的表达显示出了相当的一致性。结论 Taurolidine可通过促进GRIM-19蛋白的表达诱导Hela细胞的凋亡,且线粒体凋亡很可能是taurolidine诱导Hela细胞凋亡的重要途径。
[Abstract]:Objective to investigate the role of GRIM-19 protein in apoptosis of human cervical carcinoma Hela cells induced by taurolidine. Methods MTT assay was used to detect the survival rate of cervical cancer cell line (Hela cell line) in different dose groups, and flow cytometry was used to detect the apoptosis data of Hela cell line. The expression of GRIM-19 protein and the activity of cysteine protease (caspase) 9 were detected by Western blot and enzyme-linked immunosorbent assay (Elisa). Results MTT results showed that taurolidine inhibited Hela cell growth significantly, and flow cytometry showed that the proportion of Hela cell apoptosis in the experimental group increased significantly, and showed a significant time-course and dose-effect relationship (P0.05). Western blot analysis showed that Hela cells in the experimental group had significant apoptosis. Compared with the control group, the expression of pGRIM-19 protein in Hela cells was significantly increased (P0.05). The results of Elisa showed that the expression of pGRIM-19 in Hela cells of each experimental group was significantly higher than that of the control group (P0.05), and also showed a certain time-course and dose-response relationship. The results of Elisa showed that the expression of pGRIM-19 in Hela cells increased significantly. The expression of GRIM-19 protein was consistent with that of GRIM-19 protein. Conclusion Taurolidine can induce the apoptosis of taurolidine cells by promoting the expression of GRIM-19 protein, and mitochondrial apoptosis may be an important way to induce apoptosis of Hela cells.
【作者单位】：吉林省肿瘤医院;吉林大学公共卫生学院;吉林大学第二临床医院;
【基金】：吉林省发改委资助项目(No.2013C033)
【分类号】：R737.33

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