血管生成相关因子内皮抑素和血小板反应素-1在多囊卵巢综合征大鼠卵巢组织中的表达及意义

发布时间：2018-06-28 18:15

本文选题：多囊卵巢综合征 + 内皮抑素　；参考：《疑难病杂志》2016年11期

【摘要】：目的探讨多囊卵巢综合征(PCOS)大鼠卵巢组织中内皮抑素(ES)和血小板反应素-1(TSP-1)的表达及意义。方法 2014年12月—2015年4月于哈尔滨医科大学附属第二医院动物实验中心进行实验。选取6周龄清洁雌性SD大鼠40只,随机分为对照组20只,模型组20只。模型组予来曲唑1 mg·kg~(-1)·d~(-1)连续灌胃21 d建立PCOS大鼠模型,对照组予等剂量1%羧甲基纤维索灌胃。造模成功后处死各组大鼠留取血清样本并摘取双侧卵巢组织取材,检测血清卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E_2)、孕激素(P)及睾酮(T)水平,观察大鼠卵巢形态学及组织学变化,并采用免疫组织化学法检测卵巢组织中ES和TSP-1的表达。结果模型组大鼠卵巢体积增大,可见包膜下囊性扩张的卵泡,镜下观察可见卵巢组织结构紊乱。与对照组比较,模型组大鼠体质量和卵巢质量均明显升高(t=3.918、2.389,P=0.000、0.024);模型组血清T、LH及E_2水平均增高,而FSH及P水平均降低(t=-2.309、2.870、5.330、-7.513、7.323,P0.05或0.01);模型组ES阳性(+++)分布显著高于对照组,而TSP-1阳性(+++)分布低于对照组(U=-4.762、-4.898,P=0.000、0.002)。结论来曲唑能够成功诱导PCOS大鼠模型,ES及TSP-1在PCOS的发生发展中发挥着重要作用。
[Abstract]:Objective to investigate the expression and significance of endostatin (es) and platelet reactive hormone 1 (TSP-1) in ovarian tissues of polycystic ovary syndrome (PCOS) rats. Methods from December 2014 to April 2015, the experiment was carried out in the Animal experiment Center of the second affiliated Hospital of Harbin Medical University. Forty clean female SD rats aged 6 weeks were randomly divided into control group (n = 20) and model group (n = 20). The model group was fed with letrozole 1 mg kg ~ (-1) d ~ (-1) for 21 days to establish PCOS rat model, and the control group was given 1% carboxymethyl fiber cord at the same dose. After the model was successfully made, the rats in each group were killed to collect serum samples and to collect the samples from bilateral ovarian tissues. The levels of serum follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E _ 2), progesterone (P) and testosterone (T) were detected, and the changes of ovary morphology and histology were observed. The expression of es and TSP-1 in ovarian tissues was detected by immunohistochemistry. Results in the model group, the ovarian volume was enlarged and subcapsular cystic dilated follicles were observed. Compared with the control group, the body weight and ovarian weight of the model group were significantly higher (t = 3.918 / 2.389P = 0.0000.000 / 0.024), the serum levels of TnH and E _ 2 were increased, while the levels of FSH and P were decreased (t _ (2.309) ~ 2.8705.330-7.513137.323C P 0.05 or 0.01), the distribution of es positive in the model group was significantly higher than that in the control group (P0.05 or 0.01). The positive distribution of TSP-1 was lower than that of the control group (U-4.762 ~ 4.898P 0.0000.0002). Conclusion Letrozole can successfully induce PCOS rat model and TSP-1 plays an important role in the development of PCOS.
【作者单位】：哈尔滨医科大学附属第二医院妇产科;朝阳市中心医院妇产科;哈尔滨医科大学;
【基金】：黑龙江省自然科学基金资助项目(H201392)
【分类号】：R711.75

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