大黄素衍生物A对卵巢癌细胞迁移和侵袭的影响

发布时间：2018-06-29 13:26

本文选题：大黄素衍生物A + 抗转移　；参考：《中国现代医学杂志》2015年19期

【摘要】：目的研究大黄素衍生物A对具有不同淋巴结转移潜能的卵巢癌细胞的迁移和侵袭能力的抑制作用,并且探讨其可能的作用机制。方法四甲基偶氮唑蓝(MTT)法检测不同浓度大黄素衍生物A处理人卵巢浆液性乳头状腺癌细胞(SKOV3)和具有淋巴结定向高转移能力的亚克隆细胞(SKOV3-pm4)24 h后细胞的增殖作用。免疫荧光法观察细胞中Rac1蛋白的分布情况。不同浓度药物作用两种细胞后,Western blot检测细胞Rac1蛋白的表达量,细胞划痕实验检测细胞的迁移能力,Transwell侵袭实验测定细胞的侵袭能力。结果在一定浓度范围内大黄素衍生物A对SKOV3和SKOV3-pm4细胞的增殖均有抑制作用,24 h的半数抑制浓度(IC50)分别为23.21和34.46 mg/L。免疫荧光观察到两种细胞中Rac1蛋白均广泛分布于细胞质中,Western blot结果显示SKOV3-pm4细胞Rac1蛋白表达量高于SKOV3(P0.05)。4和8 mg/L处理组中Rac1蛋白表达较空白组明显降低。细胞划痕实验和Transwell小室侵袭实验结果显示,大黄素衍生物A处理后两种细胞的迁移和侵袭能力均受到抑制。结论大黄素衍生物A能抑制具有高淋巴结转移潜能的卵巢癌细胞的迁移和侵袭能力,其作用机制可能与下调Rac1蛋白的表达有关。
[Abstract]:Objective to study the inhibitory effect of emodin derivative A on the migration and invasion ability of ovarian cancer cells with different lymph node metastasis potential, and to explore its possible mechanism. Methods four methyl azazolium (MTT) method was used to detect different concentrations of emodin derivative A in human ovarian serous papillary adenocarcinoma cells (SKOV3) and to have the effect of drenching. The proliferation of subclone cells (SKOV3-pm4) 24 h with high metastasis ability. The distribution of Rac1 protein in the cells was observed by immunofluorescence. After two cells with different concentrations of drugs, Western blot was used to detect the expression of Rac1 protein. Cell scratch test was used to detect cell migration ability and Transwell invasion test. Results in a certain concentration, emodin derivative A could inhibit the proliferation of SKOV3 and SKOV3-pm4 cells. The median inhibitory concentration of 24 h (IC50) was 23.21 and 34.46 mg/L., respectively, and the two cells were found to be widely distributed in the cytoplasm, and Western blot results showed SKOV3-pm4. The expression of Rac1 protein was higher than that in SKOV3 (P0.05).4 and 8 mg/L treatment group, the expression of Rac1 protein was significantly lower than that in the blank group. The cell scratch test and the Transwell chamber invasion experiment showed that the migration and invasion ability of the two cells after the emodin derivative A treatment were inhibited. Conclusion the emodin derivative A can inhibit the high lymph nodes. The migration and invasion of ovarian cancer cells with metastatic potential may be related to the down-regulation of Rac1 protein expression.
【作者单位】：广西医科大学医学科学实验中心;广西医科大学附属肿瘤医院实验研究部;广西医科大学药学院;
【基金】：国家自然基金(No:81060218;81360502) 广西自然科学基金(No:2014GXNSFAA118161) 广西区域性高发肿瘤早期防治研究重点实验室自主研究项目(No:GK2014-ZZ15)
【分类号】：R737.31

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