申请试管婴儿妇女TORCH感染指标检测结果分析
本文选题:TORCH + 试管婴儿 ; 参考:《青岛大学》2017年硕士论文
【摘要】:TORCH是一组能够引起孕妇流产和胎儿感染,造成先天畸形或发育异常的几种病原体的统称,包括弓形体、风疹病毒、巨细胞病毒和单纯疱疹病毒为主的的四种病原体。发生TORCH感染的育龄妇女尤其是妊娠期,病原体会通过胎盘或产道感染胎儿,造成早产、流产、死胎、胎儿宫内发育不良、先天畸形以及新生儿中枢神经损害等后果。生殖医学科的不孕患者日渐增加,由于各种原因申请试管婴儿育龄期妇女也越来越多,TORCH感染可导致惯性流产、输卵管堵塞以及宫颈炎症等等,这些原因都是孕龄期妇女不孕的重要因素,也是申请试管婴儿的适应症。目的:目前国内尚无申请试管婴儿妇女TORCH感染调查研究,本研究对来我院生殖医学科申请试管婴儿的不孕妇女进行常规TORCH感染筛查,以了解我院申请试管婴儿的不孕妇女TORCH感染情况;目前国内也尚无与产科或妇科筛查TORCH的病人结果对比分析的研究,通过对比分析可以了解我院申请试管婴儿的不孕妇女和产科以及妇科筛查TORCH的病人感染结果的情况,从而获得更好的提示临床大夫关于TORCH感染情况,为优生优育提供更好的数据依据。方法:本文通过申请试管婴儿妇女信息采集、血清标本采集、应用LIAISON XL全自动化学发光免疫分析仪及其配套试剂检测对申请试管婴儿妇女TORCH各项Ig M和Ig G抗体检测,利用数据统计分析建立数据库;通过产科和妇科病人信息采集、血清标本采集、应用LIAISON XL全自动化学发光免疫分析仪及其配套试剂检测对申请试管婴儿妇女TORCH各项Ig M和Ig G抗体检测,利用数据统计分析建立数据库;通过数据库进行对比分析获得想要的结果。结果:(1)患者年龄主要分布在25岁~30岁、30岁~35岁和35岁~40岁区段,其在申请试管婴儿妇女所占百分比分别为32.8%、34.8%和22.8%。(2)4种病原体Ig M阳性检出结果:TOX-Ig M阳性率为0.25%,RV-Ig M阳性率为1.47%,CMV-Ig M阳性率为1.25%和HSV(Ⅰ+Ⅱ)-Ig M为7.06%。(3)4种病原体Ig G阳性检出结果:TOX-Ig G为0.93%、RV-Ig G为90.39%、CMV-Ig G为92.52%和HSV(Ⅰ+Ⅱ)-Ig G为97.6%。(4)三种病人RV+CMV+HSV-Ig G混合阳性所占比率最高,申请试管婴儿病人、妇科病人和产科病人分别为85.69%、83.72%和80.39%。三种病人各种病原体均阴性所占比率产科最高为2.34%,其次为申请试管婴儿患者为0.97%,妇科患者最低为0.47%。(5)三种病人均以TORCH-Ig M阴性为主,产科最高为95.3%,其次为妇科患者91.35%,最低为申请试管婴儿的患者90.32%;三种病人均以HSV(Ⅰ+Ⅱ)-Ig M阳性为最高,产科病人为3.16%,申请试管婴儿患者为6.81%,妇科病人为4.20%。结论:(1)我院申请试管婴儿育龄期妇女年龄以25-40岁之间为主。(2)4种病原体Ig G阳性率以弓形虫最低,其它3种均较高,单纯疱疹病毒为最高。(3)4种病原体以Ig G阳性作为分类表明以RV+CMV+HSV病原体混合感染为最高,单纯感染所占比率及阴性患者均较低。(4)Ig M阳性作为分类表明,以Ig M阴性为主,混合感染所占比率较低。(5)三种患者TORCH-Ig G筛查结果中RV+CMV+HSV混合感染以申请试管婴儿为最高,感染情况比妇科和产科患者要严重。(6)三种患者TORCH-Ig M筛查结果中申请试管婴儿的患者HSV(Ⅰ+Ⅱ)-Ig M比率最高,其它三种病原体均介于产科与妇科患者之间。
[Abstract]:TORCH is a group of pathogens that can cause abortion and fetal infection, cause congenital malformation or abnormal development of several pathogens, including Toxoplasma, rubella virus, cytomegalovirus and herpes simplex virus four pathogens. TORCH infected women of childbearing age, especially pregnancy, are infected by placenta or birth canal infection. Fetus, resulting in premature birth, abortion, stillbirth, fetal intrauterine dysplasia, congenital malformation, and neonatal central nerve damage. Infertility patients in the reproductive medical department are increasing. For various reasons, more and more women of childbearing age are applied for test tube infants, TORCH infection can lead to inertial abortion, tubal blockage and cervicitis and so on. These reasons are important factors for infertility in pregnant women and are also suitable for the application of test tube infants. Objective: at present, there is no investigation and Study on TORCH infection in test tube baby women. This study is a routine screening of TORCH infection for infertile women in the Department of reproductive medicine of our hospital to understand our application for test tube infants. At present, there is no comparative analysis of the results of the patients with obstetric or gynecologic screening of TORCH in China. Through comparative analysis, we can get a better understanding of the infection results of the patients who have applied for the infertility women and obstetrics and the gynecologic screening of TORCH in TORCH, so as to get a better indication of the clinical doctor about the TO. RCH infection conditions provide a better data basis for eugenics. Methods: by applying the information collection of the test tube baby women, the collection of serum specimens, the application of the LIAISON XL full automatic chemiluminescence immunoassay analyzer and its matching reagents to test the Ig M and Ig G antibodies of the application test tube baby women's Ig M and Ig G. The data collection of the obstetric and gynecologic patients, the collection of serum samples, the application of LIAISON XL full automatic chemiluminescence immunoassay analyzer and its matching reagents to test the TORCH Ig M and Ig G antibody of the application test tube baby women, and establish the data base by the statistical analysis of the data, and obtain the desired results through the comparative analysis of the database. Results: (1) the age of the patients was mainly distributed at the age of 25 years old ~30, 30 year old ~35 and 35 year old ~40, and the percentage of women in the application for test tube infants was 32.8%, 34.8% and 22.8%. (2) of Ig M positive results: the positive rate of TOX-Ig M was 0.25%, the positive rate of RV-Ig M was 1.47%, CMV-Ig M positive rate was 1.25% and CMV-Ig M (I + II). The positive results of 7.06%. (3) 4 pathogens were positive: TOX-Ig G was 0.93%, RV-Ig G was 90.39%, CMV-Ig G was 92.52% and HSV (I + II), -Ig G was the highest proportion of three patients (4). The application for test tube infants, gynecologic and family patients were 85.69%, 83.72% and three of the patients. The highest rate of all negative obstetrical obstetrics was 2.34%, followed by 0.97% for test tube infants, 0.97% for gynaecologic patients and 0.47%. (5) in gynecologic patients (5), with TORCH-Ig M negative, 95.3% in obstetrics, 91.35% in gynecologic patients and 90.32% for patients who applied for test tube infants; and three patients with HSV (I + II) -Ig M positive as the most High, obstetric patients 3.16%, 6.81% for test tube infants and 4.20%. for gynecologic patients: (1) the age of women of childbearing age in our hospital was 25-40 years old. (2) the Ig G positive rate of 4 pathogens was the lowest, the other 3 were higher, and the Dan Chunpao rash virus was the highest. (3) 4 pathogens were classified as Ig G positive as the classification table. RV+CMV+HSV pathogen mixed infection was the highest, the ratio of simple infection and negative patients were low. (4) Ig M positive as the classification showed that Ig M negative mainly, the ratio of mixed infection was lower. (5) the three cases of TORCH-Ig G screening results of RV+CMV+HSV mixed infection to apply for test tube infants is the highest, infection than gynecology and production. The patients were serious. (6) the rate of HSV (I + II) -Ig M was the highest in the results of TORCH-Ig M screening, and the other three pathogens were between obstetric and gynecologic patients.
【学位授予单位】:青岛大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:R714.8
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