miR-106b在卵巢浆液性癌发生发展中的作用

发布时间：2018-07-01 13:58

本文选题：卵巢浆液性癌 + miR-b　；参考：《现代妇产科进展》2015年09期

【摘要】：目的:研究miR-106b在卵巢浆液性癌中的表达及其对卵巢癌细胞增殖、侵袭的影响,并对其可能机制进行初步分析。方法:运用原位杂交和RT-PCR技术检测输卵管上皮和卵巢浆液性癌组织中miR-106b表达情况;转染miR-106b的mimics作用于SKOV3细胞,应用生长曲线、克隆形成、侵袭实验检测miR-106b对细胞增殖和侵袭的影响。结果:原位杂交实验显示,miR-106b在卵巢浆液性癌组织中的表达明显高于输卵管上皮。Real time-PCR结果证实,miR-106b在多数卵巢浆液性癌患者(n=30)组织中的表达明显高于输卵管上皮(n=8);生长曲线和克隆形成试验显示,过表达miR-106b明显促进卵巢癌细胞的增殖和转化;侵袭实验显示,miR-106b明显促进卵巢癌细胞的侵袭力。结论:miR-106b在卵巢浆液性癌组织中高表达,miR-106b促进卵巢癌细胞的增殖、克隆形成和侵袭,miR-106b在卵巢浆液性癌发生发展中可能发挥重要作用。
[Abstract]:Aim: to study the expression of miR-106b in ovarian serous carcinoma and its effect on proliferation and invasion of ovarian cancer cells. Methods: in situ hybridization and RT-PCR were used to detect the expression of miR-106b in oviduct epithelial and ovarian serous carcinoma tissues, and mimics transfected with miR-106b was applied to SKOV3 cells. The effect of miR-106 b on cell proliferation and invasion was detected by invasive assay. Results: the expression of miR-106b in ovarian serous carcinoma was significantly higher than that in oviductal epithelium. Real time-PCR results showed that the expression of miR-106b in most ovarian serous carcinoma (nm30) tissues was significantly higher than that in oviduct epithelium (nm8). Long curves and clone formation tests showed that, Overexpression of miR-106b significantly promoted the proliferation and transformation of ovarian cancer cells, and invasion assay showed that miR-106b significantly promoted the invasiveness of ovarian cancer cells. Conclusion the high expression of miR-106b in ovarian serous carcinoma can promote the proliferation of ovarian cancer cells. Clone formation and invasion of miR-106b may play an important role in the occurrence and development of ovarian serous carcinoma.
【作者单位】：山东省交通医院妇产科;山东省肿瘤医院病理科;
【分类号】：R737.31

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