miR-423、miR-205和miR-1307单核苷酸多态性与子宫颈癌的相关性研究

发布时间：2018-07-02 12:40

  本文选题：miRNA + 基因多态性　； 参考：《中国妇产科临床杂志》2017年04期

【摘要】：目的探讨mi R-423、mi R-205和mi R-1307单核苷酸多态性与宫颈癌的相关性。方法以病例-对照研究的方法收集104例宫颈癌及186例骨折、高血压等同期非肿瘤患者为对照,采用PCR-限制性酶切片段长度多态性技术对mi R-423 rs6505162位点进行基因型分析;采用PCR和多重连接酶检测反应技术对mi R-205 rs3842530和mi R-1307 rs7911488基因多态性进行分析。结果 rs6505162 CC、rs6505162 AC和rs6505162AA基因型在宫颈癌组中分布为64.4%、31.7%和3.8%,对照组分布为66.1%、30.1%和3.8%;rs3842530-/-、-/insert和inser/inser基因型在宫颈癌组中分布为56.7%、34.6%和8.7%,对照组分布为52.7%、36.0%和7.0%;rs7911488 AA、AG和GG基因型在宫颈癌组中分布为51.9%、39.4%和8.7%,对照组分布为45.1%、45.1%和9.7%。各位点基因型分布比较,差异均无统计学意义(P0.05)。进一步按年龄分层分析显示,≤50岁和50岁不同年龄组各基因型分布在对照组和宫颈癌组中比较,差异无统计学意义(P0.05)。结论 mi R-423 rs6505162、mi R-205 rs3842530和mi R-1307 rs7911488基因多态性可能与宫颈癌发生无关。
[Abstract]:Objective to investigate the association of single nucleotide polymorphisms (SNP) of mi R 423 M M R 205 and mi R 1307 with cervical cancer. Methods A case-control study was conducted in 104 patients with cervical cancer, 186 patients with fracture, hypertension and other non-tumor patients. The genotypes of miR-423 rs6505162 locus were analyzed by PCR- restriction fragment length polymorphism (PCR- restriction fragment length polymorphism). The polymorphism of mi R-205 rs3842530 and mi R-1307 rs7911488 gene was analyzed by PCR and multiplex ligase assay. Results the distribution of AC and rs6505162AA genotypes of rs6505162 CCS rs6505162 in the cervical cancer group was 64.7% and 3.8%, and that in the control group was 66.1% and 3.8G%, respectively. The distribution of rs3842530-r-rinsert and inser/inser genotype in the cervical cancer group was 56.734.6% and 8.7%, respectively. In the control group, the distribution of rs7911488 AAAG and GG genotypes was 52.7% and 7.0% respectively. The distribution was 39.4% and 8.7% in the control group and 45.1% and 9.7% in the control group. There was no significant difference in genotypic distribution among the points (P0.05). Further age stratification analysis showed that the genotypes of different age groups 鈮，

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