顺铂对子宫内膜癌Ishikawa细胞增殖及自噬的影响

发布时间：2018-07-02 13:50

本文选题：顺铂 + Ishikawa细胞　；参考：《实用医学杂志》2017年04期

【摘要】：目的:观察顺铂对子宫内膜癌Ishikawa细胞的增殖及自噬的影响。方法:MTS法检测不同剂量顺铂对Ishikawa细胞增殖的作用。透射电镜观察自噬泡形成,应用Western blot检测LC3及Beclin1的积累,免疫荧光检测LC3的荧光聚集情况。结果:(1)10μg/m L顺铂对Ishikawa细胞的增殖有抑制作用,随着时间及浓度的增加,细胞增殖的抑制作用明显增加(P0.01);(2)透射电镜显示顺铂处理后子宫内膜癌细胞发生自噬,随着时间剂量增加,Western blot检测LC3表达增加,Becline1表达无明显变化;(3)与对照组相比,20μg/m L-12 h组的自噬体及LC3表达增加;20μg/m L-24 h组较12 h组的自噬体及LC3表达增加。结论:顺铂对Ishikawa细胞增殖有明显抑制作用,呈时间剂量依赖性;顺铂诱导Ishikawa细胞发生自噬,呈时间剂量依赖性。自噬相关基因LC3的变化可能为顺铂诱导子宫内膜癌自噬分子机制之一。
[Abstract]:Aim: to observe the effect of cisplatin on proliferation and autophagy of endometrial carcinoma Ishikawa cells. Methods the effects of different doses of cisplatin on the proliferation of Ishikawa cells were detected by the method of: MTS. The formation of autophagy was observed by transmission electron microscope. The accumulation of LC3 and Beclin1 was detected by Western blot and the fluorescence aggregation of LC3 was detected by immunofluorescence. Results: (1) 10 渭 g / mL cisplatin inhibited the proliferation of Ishikawa cells. With the increase of time and concentration, the inhibitory effect of cisplatin on the proliferation of Ishikawa cells was significantly increased (P0.01); (2) transmission electron microscope showed that cisplatin induced autophagy in endometrial cancer cells. (3) compared with the control group, the autophagy and LC3 expression in the 20 渭 g / mL 12 h group increased significantly compared with the control group, and the expression of autophagy and LC3 in the 20 渭 g / mL 24 h group was higher than that in the 12 h group. Conclusion: cisplatin can inhibit the proliferation of Ishikawa cells in a time-dose dependent manner, and cisplatin can induce autophagy of Ishikawa cells in a time-dose-dependent manner. The change of autophagy associated gene LC3 may be one of the mechanisms of cisplatin induced autophagy in endometrial carcinoma.
【作者单位】：广州医科大学第三附属医院;南方医科大学珠江医院;
【基金】：广州市卫生局一般引导项目资助项目(编号:2015A013010068)
【分类号】：R737.33

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