孕期酒精暴露对新生大鼠延髓基本节律性呼吸放电的作用

发布时间：2018-07-06 07:38

本文选题：孕期酒精暴露 + 基本节律性呼吸放电　；参考：《南方医科大学学报》2015年04期

【摘要】：目的探讨孕期酒精暴露对子代新生大鼠延髓脑片基本节律性呼吸放电(rhythmic respiratory discharge activity,RRDA)的作用。方法制作包含面神经后核内侧区(the medial region of the nucleus retrofacialis,m NRF)并保留舌下神经根的新生大鼠离体延髓脑片标本,给予灌流改良的Kreb's液(modified Kreb's solution,MKS),使用吸附电极记录延髓脑片腹侧面舌下神经根RRDA,分析RRDA的变化。实验分为5组:对照组、4%酒精暴露组、6%酒精暴露组、8%酒精暴露组和10%酒精暴露组,研究孕期不同浓度酒精暴露对RRDA的作用。结果对照组、4%酒精暴露组、6%酒精暴露组、8%酒精暴露组各组组内RRDA在50min内吸气时程(TI)、呼吸频率(RF)、放电积分幅度(IA)无统计学差异,10%酒精暴露组RRDA节律不规整;与对照组相比,4%~10%酒精暴露组的RRDA随酒精浓度增加逐渐降低,TI缩短、RF下降、IA减弱。结论孕期酒精暴露抑制子代新生大鼠延髓脑片舌下神经根RRDA,这可能是孕期酒精暴露抑制子代呼吸功能的基础。
[Abstract]:Objective to investigate the effects of alcohol exposure during pregnancy on the basic rhythmic respiratory discharge (rhythmic respiratory discharge) activity in neonatal rat medulla oblongata. Methods New born rats with (the medial region of the nucleus retrofacialism NRF in the medial area of the posterior nucleus of the facial nerve and preserved the roots of the hypoglossal nerve were made in vitro medulla oblongata slices. The modified Krebos-solutionMKS solution was perfused to record the hypoglossal nerve root of ventral side of medulla oblongata with adsorption electrode, and to analyze the changes of RRDA in the hypoglossal nerve root of the ventral side of medulla oblongata. The experiment was divided into five groups: control group with 4% alcohol exposure group with 6% alcohol exposure group with 8% alcohol exposure group and 10% alcohol exposure group. The effects of different concentrations of alcohol exposure on RRDA during pregnancy were studied. Results there was no significant difference in the inspiratory duration (TI), respiratory rate (RF) and discharge integral amplitude (IA) between the control group (4% alcohol exposure group, 6% alcohol exposure group) and 8% alcohol exposure group (8% alcohol exposure group). Compared with the control group, the RRDA of 10% alcohol exposure group decreased gradually with the increase of alcohol concentration. Conclusion Alcohol exposure during pregnancy inhibits the respiratory function of the offspring of neonatal rats by inhibiting the RRDAs of hypoglossal nerve roots in the medulla oblongata of neonatal rats.
【作者单位】：新乡医学院基础医学院生理学教研室;新乡医学院基础医学院机能学实验室;
【基金】：河南省科技厅重点攻关项目(102102310156) 河南省教育厅自然科学项目(2010B180024)
【分类号】：R714

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