Ikaros的3种亚型对人卵巢癌SKOV3细胞增殖的影响

发布时间：2018-07-06 07:48

本文选题：Ikaros亚型 + 卵巢癌　；参考：《中国病理生理杂志》2015年08期

【摘要】：目的:探讨Ikaros的3种亚型对人卵巢癌SKOV3细胞增殖的影响。方法:利用逆转录病毒转染人卵巢癌SKOV3细胞,分别表达Ikaros的3种亚型(IK1、IK2和IK6);采用CCK-8法分析表达不同亚型后SKOV3细胞的增殖能力;流式细胞术检测细胞周期的改变;Western blot检测细胞周期相关蛋白的表达。结果:CCK-8结果显示IK1和IK2能明显抑制SKOV3细胞的增殖;细胞周期结果表明IK1和IK2能诱导SKOV3细胞发生G1期阻滞,IK6则对SKOV3细胞的增殖能力和细胞周期则无明显影响;Western blot检测结果显示,IK1和IK2明显降低cyclin D1和cyclin D2蛋白的表达水平,同时升高p21蛋白的表达水平。IK6则对SKOV3细胞的cyclin D1、cyclin D2及p21蛋白表达水平无明显改变。结论:IK1和IK2这2种亚型能明显抑制卵巢癌SKOV3细胞的增殖,其机制可能是由于IK1和IK2能降低细胞周期促进蛋白cyclin D1和cyclin D2的表达及增加细胞周期抑制蛋白p21的表达,从而诱导细胞周期发生G1期阻滞。而IK6亚型对SKOV3细胞增殖能力及细胞周期无明显影响。
[Abstract]:Aim: to investigate the effects of three Ikaros subtypes on the proliferation of human ovarian cancer SKOV3 cells. Methods: human ovarian cancer SKOV3 cells were transfected with retrovirus and expressed three Ikaros subtypes (IK1K2 and Ik6) respectively, and the proliferative ability of SKOV3 cells after expressing different subtypes was analyzed by CCK-8 method. Flow cytometry was used to detect the change of cell cycle. Western blot was used to detect the expression of cell cycle associated protein. Results the results of 1: CCK-8 showed that IK1 and IK2 could significantly inhibit the proliferation of SKOV3 cells. The results of cell cycle showed that I K1 and I K2 could induce G1 phase arrest of SKOV3 cells and Ik6 had no effect on the proliferation and cell cycle of SKOV3 cells. The results of Western blot showed that Ik1 and Ik2 significantly decreased the expression of cyclin D1 and cyclin D2 proteins in SKOV3 cells. The expression level of cyclin D1cyclin D2 and p21 protein in SKOV3 cells was not significantly changed by increasing the expression level of p21 protein. Conclusion the proliferation of ovarian cancer SKOV3 cells can be significantly inhibited by the two subtypes: 1: IK1 and IK2. The mechanism may be that IK1 and IK2 can decrease the expression of cyclin D1 and cyclin D2 and increase the expression of cell cycle inhibitor p21. Thus, G 1 arrest was induced in cell cycle. The proliferation and cell cycle of SKOV3 cells were not affected by IK6 subtype.
【作者单位】：温州医科大学检验医学院、生命科学学院;
【基金】：国家自然科学基金资助项目(No.81400125) 浙江省自然科学基金资助项目(No.LQ13H080002) 教育部博士点新教师类资助项目(No.2013321120003) 温州医科大学科研启动资金资助项目(No.QTJ12009)
【分类号】：R737.31

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