NF-κB和MMP-9在亚临床甲减孕鼠胎盘中的表达及意义

发布时间：2018-07-11 13:34

本文选题：亚临床甲减 + 胎盘　；参考：《南京医科大学学报(自然科学版)》2017年05期

【摘要】：目的:通过研究核转录因子-κB(Nuclear Kappa B,NF-κB)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在亚临床甲减孕鼠胎盘组织中的变化,分析亚临床甲减孕鼠早期先兆流产的发生机制。方法:采用免疫组织化学法、Western blot检测NF-κB和MMP-9在亚临床甲减孕鼠胎盘组织中(E10)和正常组中的表达情况,分析二者与亚临床甲减孕鼠早期先兆流产的相关性。结果:NF-κB和MMP-9在亚临床甲减孕鼠胎盘组织中的阳性表达率分别为16.7%和50.0%,而在正常组胎盘组织中的阳性率分别为83.3%和100.0%,差异均有统计学意义;Western blot检测结果与免疫组织化学检测结果基本一致。结论:NF-κB和MMP-9在亚临床甲减孕鼠胎盘组织中低表达,导致滋养细胞侵袭力下降,对判断妊娠期亚临床甲减早期先兆流产发生机制具有重要指导意义。
[Abstract]:Objective: to study the changes of nuclear Kappa BnNF- 魏 B and matrix metalloproteinase-9 (MMP-9) in placenta of subclinical hypothyroidism rats, and to analyze the mechanism of early threatened abortion in subclinical hypothyroidism mice. Methods: the expression of NF- 魏 B and MMP-9 in placenta of subclinical hypothyroidism rats (E10) and normal group were detected by immunohistochemical method and Western blot, and the correlation between them and early threatened abortion in subclinical hypothyroidism rats was analyzed. Results the positive expression rates of 10% NF- 魏 B and MMP-9 in placenta of subclinical hypothyroidism mice were 16.7% and 50.0%, respectively, while those in normal placenta tissues were 83.3% and 100.0.The differences were statistically significant. The results of chemical detection are basically the same. Conclusion the low expression of 10% NF- 魏 B and MMP-9 in placenta of subclinical hypothyroidism rats leads to the decrease of trophoblastic invasiveness, which is of great significance in judging the mechanism of threatened abortion in the early stage of subclinical hypothyroidism in pregnancy.
【作者单位】：南京中医药大学附属常州市中医医院妇科;南京中医药大学附属苏州市中医医院生殖医学科;
【基金】：苏州市科技局科技发展计划(SYSD2012115,SYSD2013126) 苏州市卫生局科技项目(LCZX201512)
【分类号】：R714.256

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