EZH2蛋白在卵巢上皮性肿瘤中的表达及其作用

发布时间：2018-07-13 18:15

【摘要】：目的观察EZH2在不同卵巢组织中的表达,探讨其在肿瘤发展中可能发挥的作用。方法采用免疫组织化学染色检测15例正常卵巢组织、32例卵巢良性肿瘤、28例卵巢交界性肿瘤及50例卵巢癌中EZH2蛋白的表达,其中15例正常卵巢组织、23例卵巢良性肿瘤、23例交界性肿瘤及25例卵巢癌行RT-PCR检测。结果 1EZH2蛋白在卵巢癌细胞核中的表达明显高于正常卵巢组织(P0.01)、卵巢良性肿瘤(P0.01)及交界性肿瘤(P0.05),且与肿瘤的分级和分期具有相关性(P0.01,P0.05);2 EZH2蛋白在卵巢肿瘤组织的细胞质中亦有表达,且良性肿瘤和交界性肿瘤中的表达高于卵巢癌(P均0.05);3 EZH2蛋白在卵巢癌细胞核中的表达与Ki-67呈正相关性(r=0.40,P0.01)。结论卵巢肿瘤组织细胞核及细胞质中均有EZH2蛋白的表达,且卵巢癌细胞核中EZH2蛋白的表达与肿瘤的分期及分级具有相关性,并参与细胞增殖。
[Abstract]:Objective to investigate the expression of EZH2 in different ovarian tissues and its possible role in tumor development. Methods Immunohistochemical staining was used to detect the expression of EZH2 protein in 32 cases of benign ovarian tumors and 28 cases of ovarian borderline tumors and 50 cases of ovarian cancer. Among them 15 cases of normal ovarian tissues 23 cases of benign ovarian tumors 23 cases of borderline tumors and 25 cases of ovarian cancer were detected by RT-PCR. Results 1the expression of EZH2 protein in the nucleus of ovarian cancer was significantly higher than that in normal ovarian tissue (P0.01), ovarian benign tumor (P0.01) and borderline tumor (P0.05). Also expressed in the cytoplasm, The expression of EZH2 in benign and borderline tumors was significantly higher than that in ovarian carcinoma (P 0.05), and the expression of Ki-67 was positively correlated with Ki-67 (r = 0.40, P 0.01). Conclusion EZH2 protein is expressed in the nucleus and cytoplasm of ovarian tumor, and the expression of EZH2 protein is correlated with the stage and grade of the tumor, and it is involved in cell proliferation.
【作者单位】：山东大学附属省立医院妇产科;山东大学附属省立医院病理科;山东大学附属省立医院手术麻醉科;
【基金】：国家自然科学基金(81172482) 中国博士后科学基金(2012M520201)
【分类号】：R737.31

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