培美曲塞联合β-榄香烯对宫颈癌HeLa细胞增殖和凋亡的影响

发布时间：2018-07-15 18:23

【摘要】：目的培美曲塞和β-榄香烯均可抑制肿瘤细胞的生长。文中探讨培美曲塞联合β-榄香烯对宫颈癌HeLa细胞增殖和凋亡的影响。方法体外培养宫颈癌HeLa细胞,将培美曲塞(浓度为38、76、152、228、304μg/m L)单独作用于HeLa细胞后24、48 h用MTT比色法检测细胞的增殖情况,实验分β-榄香烯组(125μg/m L)、培美曲塞组(76μg/m L)、联合用药组(培美曲塞76μg/m L,β-榄香烯125μg/m L)及空白对照组(0μg/m L),24 h后用MTT法检测细胞的增殖情况,用流式细胞术检测24 h细胞的凋亡率。结果 MTT法显示,培美曲塞浓度在38、76、152、228、304μg/m L梯度范围内对HeLa细胞有抑制作用,随浓度增加,抑制率增强。38、76、152、228、304μg/m L的培美曲塞作用24后,增殖抑制率分别为(7.24±3.78)%、(7.94±4.37)%、(11.10±2.86)%、(15.88±3.38)%、(16.52±2.54)%;其中38、76、152、228μg/m L培美曲塞抑制率两两比较的差异有统计学意义(P0.05)。联合用药组24 h抑制率[(49.95±5.76)%]高于β-榄香烯组[(24.36±5.59)%]和培美曲塞组[(10.69±1.37)%],差异有统计学意义(P0.01)。结论培美曲塞与β-榄香烯联合应用可抑制HeLa细胞的增殖,协同促进HeLa细胞的凋亡。
[Abstract]:Objective both pemetrexed and 尾-elemene can inhibit the growth of tumor cells. To investigate the effects of pemetrexide combined with 尾 -elemene on the proliferation and apoptosis of cervical cancer HeLa cells. Methods Cervical cancer HeLa cells were cultured in vitro. The proliferation of HeLa cells was detected by MTT colorimetry at 24 h after treatment with pemetrexil (3876 152228304 渭 g / mL) alone. The cells were divided into 尾 -elemene group (125 渭 g / mL), pemetrexide group (76 渭 g / mL), combination group (pemetrexide 76 渭 g / mL, 尾 -elemene 125 渭 g / mL) and control group (0 渭 g / mL) for 24 h. Results MTT assay showed that pemetrexed had inhibitory effect on HeLa cells in the gradient range of 38,76152228304 渭 g / mL. With the increase of concentration, the inhibition rate increased after 24 渭 g / ml of pemetrexed. The inhibition rates of proliferation were (7.24 卤3.78), (7.94 卤4.37), (11.10 卤2.86), (15.88 卤3.38), (16.52 卤2.54), respectively, in which the inhibition rate of pemetrexed was significantly higher than that of pemetrexil (P 0.05), and the inhibition rate was (7.24 卤3.78), (7.94 卤4.37), (11.10 卤2.86), (15.88 卤3.38), (16.52 卤2.54), respectively. The 24 h inhibition rate in combination group [(49.95 卤5.76)%] was significantly higher than that in 尾 -elemene group [(24.36 卤5.59)%] and pemetrexide group [(10.69 卤1.37)%] (P0.01). Conclusion the combination of pemetrexide and 尾 -elemene can inhibit the proliferation of HeLa cells and promote the apoptosis of HeLa cells.
【作者单位】：第四军医大学;解放军68222部队卫生队;第四军医大学唐都医院中医科暨中西医结合肿瘤科;第四军医大学西京医院放疗科;
【基金】：国家自然科学基金(81072973) 陕西省中医管理局中医药科研课题(lc70)
【分类号】：R737.33

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