陕西省商洛地区HPV感染状况和分型特征

发布时间：2018-07-21 17:07

【摘要】：目的:研究陕西省商洛地区妇女人乳头瘤病毒(human papillomavirus,HPV)的感染状况和亚型分布,为HPV分子流行病学和宫颈癌的防治提供依据。方法:选择2012年6月至2014年7月在商洛中心医院体检、门诊就诊的937例患者的宫颈脱落细胞标本,采用聚合酶链反应(PCR)体外扩增和脱氧核糖核酸(DNA)反向点杂交相结合的DNA芯片技术进行HPV基因分型检测。结果:HPV检出率937例标本中HPV感染566例,总感染率为60.41%(566/937),其中高危型感染病例总检出率为51.44%(482/937);低危型感染总检出率26.47%(248/937);感染年龄分布以61岁年龄段感染率最高,为88.89%,其次是≤20岁年龄段(67.86%),51~60岁年龄段(62.90%),41~50岁年龄段(60.29%),各个年龄组的感染率没有统计学差异(P0.05)。23种HPV基因亚型中,高危感染中以16型最高,占26.58%(277/1042),其次是58型,占8.93%(93/1024),66型,占6.53%(68/1024),低危感染中以6型最高,占12.38%(129/1024),其次是43型,占4.32%(45/1024),42型,占4.22%(44/1024)。单一型别占总感染例数的50.53%(286/566),多重感染以二重感染最多,占总感染例数的12.92%(158/566)。结论:陕西省商洛地区HPV感染率高,主要亚型为HPV16、HPV58、HPV66,提示HPV亚型的分析对疫苗的研发和宫颈癌的筛查及防治有指导意义。
[Abstract]:Objective: to study the infection status and subtype distribution of human papillomavirus in Shangluo area, Shaanxi Province, and to provide evidence for the molecular epidemiology and prevention and treatment of cervical cancer. Methods: from June 2012 to July 2014, 937 specimens of cervical exfoliated cells were collected from 937 patients who underwent physical examination in Shangluo Central Hospital. HPV genotyping was detected by polymerase chain reaction (PCR) amplification in vitro and DNA reverse dot hybridization. Results among 937 specimens, 566 cases were infected with human papillomavirus (HPV), the total infection rate was 60.41% (566 / 937), among which 51.44% (482 / 937) were high risk type, 26.47% (248 / 937) were low risk type, and the highest was 61 years old. It was 88.89, followed by 鈮，

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