多囊卵巢综合征大鼠AMH表达与胰岛素抵抗的相关性研究

发布时间：2018-07-28 06:48

【摘要】：目的:对多囊卵巢综合征(Polycystic ovary syndrome,PCOS)模型大鼠与卵巢局部及单卵泡中抗苗勒管素(Anti-mullerian hormone,AMH)表达与胰岛素抵抗(Insulin resistance,IR)之间的相关性进行研究。方法:采用DHEA皮下注射造模建立PCOS大鼠模型,然后计算胰岛素抵抗稳态模型(Homeostasis model assessment of insulin resistance,HOMA-IR)值,根据HOMA-IR值将所筛选对象分成单纯PCOS组,PCOS合并IR组和对照组,采用免疫组化法检测模型鼠、正常鼠血清AMH的表达及单卵泡及卵巢局部大鼠胰岛素受体及AMH表达。结果:PCOS大鼠无论是否合并IR LH、T、Flns及HOMA-IR明显高于对照组,两组差异有统计学意义(P0.05);而PCOS合并IR组T、Flns及HOMA-IR较对照组明显升高,两组差异亦有统计学意义(P0.05)。经过Pearson相关性分析结果显示卵巢局部AMH表达强度与胰岛素受体表达强度无明显相关性(r=0.015,P0.05);而卵巢局部AMH表达与血清胰岛素明显相关性(r=0.671,P=0.003);单卵泡AMH表达强度与胰岛素受体表达强度无明显相关性(r=0.011,P0.05)。而对血清AMH的表达与HOMA-IR进行Pearson相关性分析结果显示:HOMA-IR与血清AMH之间存在明显的正相关性(r=0.683,P=0.001)。结论:PCOS大鼠AMH与血清胰岛素水平呈正相关,卵巢局部AMH表达与胰岛素受体有正相关性,而单卵泡AMH表达与胰岛素受体无明显相关性,对于是否与高雄激素有关,还需要进一步研究。
[Abstract]:Aim: to study the relationship between the expression of Anti-mullerian hormonein (Anti-mullerian) and Insulin resistance (IR) in the ovary and single follicle of (Polycystic ovary syndrome model rats with polycystic ovary syndrome (PCOS). Methods: the PCOS rat model was established by subcutaneous injection of DHEA, and then the (Homeostasis model assessment of insulin resistance homeostasis (HOMA-IR) value was calculated. According to the HOMA-IR value, the selected subjects were divided into simple PCOS group with IR group and control group. The expression of AMH in serum and the expression of insulin receptor and AMH in single follicle and ovary were detected by immunohistochemical method in model rats and normal rats. Results compared with the control group, there was a significant difference between the two groups (P0.05), while the PCOS combined with IR group was significantly higher than that of the control group (P0.05), while the PCOS combined with IR group was significantly higher than that of the control group (P0.05). The results of Pearson correlation analysis showed that there was no significant correlation between the intensity of AMH expression and insulin receptor expression in ovary (r 0.015 P 0.05), but there was a significant correlation between AMH expression in ovary and serum insulin (r 0.671P 0. 003), and the AMH expression intensity in single follicle was significantly correlated with insulin. There was no significant correlation between the intensity of receptor expression (r = 0.011, P 0.05). The Pearson correlation analysis of serum AMH expression and HOMA-IR showed that there was a significant positive correlation between serum AMH and serum HOMA-IR (r = 0.683P0. 001). Conclusion there is a positive correlation between AMH and serum insulin level, a positive correlation between AMH expression in ovary and insulin receptor, but no significant correlation between AMH expression in single follicle and insulin receptor, and whether it is related to hyperandrogen. Further research is needed.
【作者单位】：深圳市龙岗区妇幼保健院生殖内分泌科;哈尔滨职工医学院解剖教研室;
【分类号】：R711.75

【参考文献】