HPV16E6和hTERT在宫颈癌致癌机制中的作用研究

发布时间：2018-07-28 11:30

【摘要】：目的观察和分析人乳头瘤病毒16(HPV16)E6蛋白和人端粒酶逆转录酶(h TERT)在宫颈癌致癌机制中的作用。方法选取220例宫颈病变标本,根据病理诊断将其分为宫颈炎症(38例)、宫颈上皮内瘤变(CIN,118例),宫颈鳞癌(SCC,36例)和宫颈腺癌(ADC,28例)。对标本中的各HPV亚型感染情况、HPV16 E6蛋白和h TERT蛋白表达情况进行检测和比较。结果在本组宫颈病变组织标本中,共有146例检出感染HPV,感染率为66.4%,其中,HPV16的感染率最高,占31.8%,占全部感染标本的47.9%;宫颈炎与CIN标本中的HPV16 E6蛋白表达阳性率均显著低于SCC标本或ADC标本(χ2=30.950、28.663、65.265、59.598,P0.05),宫颈炎标本中的h TERT蛋白表达阳性率显著低于其它病变类型(χ2=6.553、62.870、62.011,P0.05),CIN标本中的h TERT蛋白表达阳性率显著低于SCC标本或ADC标本(χ2=72.715、69.935,P0.05);HPV16 E6蛋白表达评分与SCC标本的分化程度(rs=-0.635)、ADC标本的分化程度(rs=-0.577)呈负相关关系(P0.05),hTERT蛋白表达评分与CIN标本的分期(rs=0.628)呈正相关关系而与SCC标本的分化程度(rs=-0.758)、ADC标本的分化程度(rs=-0.845)呈负相关关系(P0.05)。结论HPV感染与各类宫颈病变的发生具有相关性,HPV16 E6蛋白和h TERT蛋白的高表达可能在宫颈癌前病变和宫颈癌的发生、进展过程中发挥着重要的作用。
[Abstract]:Objective to investigate the role of human papillomavirus (HPV16) E6 protein and human telomerase reverse transcriptase (h TERT) in carcinogenesis of cervical cancer. Methods 220 cases of cervical lesions were divided into cervical inflammation (38 cases), cervical intraepithelial neoplasia (CINN 118 cases), squamous cell carcinoma of cervix (SCC) 36 cases and adenocarcinoma of cervix (ADCN 28 cases) according to pathological diagnosis. The expression of HPV16 E6 protein and h TERT protein in different HPV subtypes were detected and compared. Results among the specimens of cervical lesions, 146 cases were infected with HPV16, the infection rate was 66.4%, and the infection rate of HPV16 was the highest. The positive rate of HPV16 E6 protein expression in cervicitis and CIN specimens was significantly lower than that in SCC and ADC specimens (蠂 2 / 30.950 ~ 28.663n = 65.265nb / 59.598P 0.05), and the positive rate of h TERT protein expression in cervicitis specimens was significantly lower than that in other pathological types (蠂 2 / 6.553N 62.870 / 62.011P). The positive rate of h TERT protein expression in this study was significantly lower than that in SCC or ADC specimens (蠂 2 / 72.715 / 69.935 / P). There was a negative correlation between HPV16 E6 protein expression score and the differentiation degree (rs=-0.577) of SCC specimens (P0.05) and CIN staging (rs=0.628). There was a positive correlation and a negative correlation between the degree of differentiation (rs=-0.758) and the degree of differentiation (rs=-0.845) of SCC specimens (P0.05). Conclusion the high expression of HPV16 E6 protein and h TERT protein may play an important role in the occurrence and progression of cervical carcinoma and precancerous lesions.
【作者单位】：青海省西宁市第一人民医院妇科;
【分类号】：R737.33

【相似文献】