上皮性卵巢癌中CD133与EMT相关因子表达的关系

发布时间：2018-07-28 16:08

【摘要】：目的探讨上皮性卵巢癌(Epithelial ovarian cancer,EOC)中CD133蛋白及上皮-间质转化(Epithelial mesenchymal transition,EMT)相关因子Snail、E-cadherin蛋白表达的关系及其功能意义。方法采用免疫组织化学ElivisionTMplus法检测150例EOC和50例良性上皮性卵巢肿瘤中CD133、Snail和E-cadherin蛋白的表达情况。结果良性上皮性卵巢肿瘤组织中CD133、Snail和E-cadherin蛋白的表达阳性率分别为10%、8.0%和70%,在EOC组织中3者表达分别为58.7%、60.7%和32.7%,差异有统计学意义(P0.05)。CD133、Snail和E-cadherin的表达与EOC的腹腔脏器和淋巴结转移以及FIGO分期有关(P0.01);E-cadherin蛋白的表达与Snail及CD133蛋白的表达均呈负相关关系(r分别为-0.545和-0.570,P均0.01),CD133蛋白的表达与Snail蛋白的表达呈正相关关系(r=0.599,P0.01)。Kaplan-Meier生存分析表明,CD133和Snail的过表达均与患者的生存率有关,阳性表达的患者生存率明显低于阴性者(P0.05);而随着E-cadherin蛋白表达水平的降低EOC患者生存率明显下降(P0.05)。多因素分析表明FIGO分期、CD133、Snail和E-cadherin的表达是影响EOC根治术后患者预后的独立因素(P0.05)。结论 CD133和Snail表达的升高以及E-cadherin表达的降低与EOC的侵袭、转移和预后等因素相关,这些指标的联合检测有可能作为评估EOC患者临床预后的重要指标。
[Abstract]:Objective to investigate the relationship between the expression of CD133 protein and the expression of Snail-E-cadherin protein in epithelial ovarian carcinoma (Epithelial ovarian cancer-EOC) and the related factor of epithelial-interstitial transformation (EMTs) (Epithelial mesenchymal transition.Methods the expression of Snail-E-cadherin protein and its functional significance were investigated. Methods Immunohistochemical ElivisionTMplus method was used to detect the expression of CD133 snail and E-cadherin protein in 150 cases of EOC and 50 cases of benign epithelial ovarian tumors. Results the positive rates of CD133Snail and E-cadherin protein in benign epithelial ovarian tumors were 10% and 70%, respectively. In EOC tissues, the positive rates were 58.7% and 32.7%, respectively. The difference was statistically significant (P0.05). The expression of CD133 snail and E-cadherin was significantly higher than that of EOC in abdominal organs and lymph nodes. The expression of E-cadherin was negatively correlated with the expression of Snail and CD133 (r = -0.545 and -0.570 P0.01, respectively). The expression of CD133 was positively correlated with the expression of Snail (r = 0.599, P0.01). Kaplan-Meier survival analysis showed that CD133 and Snail were positively correlated with the expression of CD133 and Snail. Overexpression was associated with survival rate. The survival rate of patients with positive expression was significantly lower than that of patients with negative expression (P0.05), but with the decrease of E-cadherin protein expression level, the survival rate of patients with EOC decreased significantly (P0.05). Multivariate analysis showed that the expression of CD133 snail and E-cadherin in FIGO staging was an independent factor influencing the prognosis of EOC patients after radical operation (P0.05). Conclusion the increased expression of CD133 and Snail and the decrease of E-cadherin expression are related to the invasion, metastasis and prognosis of EOC. The combined detection of these markers may be an important index to evaluate the clinical prognosis of EOC patients.
【作者单位】：蚌埠医学院第一附属医院临床病理科;蚌埠医学院病理学教研室//安徽省感染与免疫重点实验室;
【基金】：安徽省高等学校省级优秀青年人才基金项目(2012SQRL094) 蚌埠医学院自然科学类重点项目(BYKY1416ZD)
【分类号】：R737.31

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