蜕膜基质细胞培养液对滋养细胞增殖、凋亡及浸润的影响及对浸润作用机制的研究

发布时间：2018-08-03 07:30

【摘要】：目的:探讨早孕蜕膜基质细胞培养液(DSCM)对滋养细胞增殖、凋亡以及浸润能力的影响及对浸润功能的作用机制。方法:原代分离、培养并鉴定人早孕蜕膜基质细胞,收集并制备成不同浓度的DSCM,采用噻唑兰(MTT)、Annexin V-FITC、Transwell方法检测不同浓度的DSCM对滋养细胞增殖、凋亡、浸润能力的影响,采用RT-PCR及明胶酶谱法检测滋养细胞基质金属蛋白酶(MMPs)的改变。结果:得到纯度大于90%的蜕膜基质细胞。随着DSCM的浓度增高(5%DSCM,20%DSCM,50%DSCM,100%DSCM)细胞的吸光度值增高、凋亡率减少、浸润细胞数增多(P0.05)。不同浓度的DSCM组MMP-2及MMP-9 mRNA、蛋白的变化与对照组相比差异均有统计学意义(P0.05)。结论:早孕DSCM可以促进滋养细胞增殖,抑制凋亡,并通过上调滋养细胞对MMP-2与MMP-9的表达来促进其浸润功能。
[Abstract]:Aim: to investigate the effect of (DSCM) on the proliferation, apoptosis and infiltration of trophoblast cells and its mechanism. Methods: primary human decidua stromal cells were isolated, cultured and identified. Different concentrations of DSCMs were collected and prepared. The effects of different concentrations of DSCM on the proliferation, apoptosis and infiltration of trophoblast cells were detected by (MTT) Annexin V-FITCX Transwell method. The changes of matrix metalloproteinase (MMPs) in trophoblasts were detected by RT-PCR and gelatinase assay. Results: decidual stromal cells with purity more than 90% were obtained. With the increase of DSCM concentration, the absorbance of the cells increased, the apoptosis rate decreased and the number of infiltrating cells increased (P0.05). The changes of MMP-2 and MMP-9 mRNAs and proteins in DSCM group were significantly different from those in control group (P0.05). Conclusion: DSCM can promote the proliferation of trophoblast, inhibit apoptosis, and promote the infiltration function by upregulating the expression of MMP-2 and MMP-9 in trophoblast.
【作者单位】：第四军医大学唐都医院;
【基金】：国家自然科学基金(编号:81471474) 唐都医院创新基金(编号:2015LCYJ013)
【分类号】：R714

【参考文献】