Livin、XIAP在子宫内膜样腺癌中的表达及相关性研究
[Abstract]:Objective: endometrial carcinoma (Endometrial Carcinoma) is a common malignant tumor in the female reproductive system, and cervical cancer, and ovarian cancer is called the three largest killer of women. Endometrioid adenocarcinoma is the most common histologic type. Its development involves apoptosis and is a complex process. The family of apoptosis suppressor protein (IAPs) family Members XIAP and Livin have inhibition of cysteine asparagus (Caspase), regulate cell apoptosis, cell division and proliferation, and play a key role in the development of malignant tumors. This experiment adopts an immunohistochemical method for normal endometrium, endometrium atypical hyperplasia and uterus. The expression of XIAP and Livin in the adenocarcinoma of the membrane was detected, and the relationship between the development of XIAP and Livin and endometrioid adenocarcinoma and the possible relationship between the two were discussed, and the theoretical basis for the clinical diagnosis and treatment of endometrial cancer was provided to improve the survival rate of the cancer patients. Methods: this experiment selected Hebei medicine. 40 cases of endometrioid adenocarcinoma, 40 specimens of endometrium hyperplasia confirmed by pathology, and 40 cases of endometrium adenocarcinoma confirmed by pathology in October 2013 -2016 years, and 40 specimens of endometrium in normal hyperplastic period of hysterectomy or hysteromyoma were selected as control. The immunohistochemical SP method was used to detect the expression of XIAP and Livin in the three groups. The data were statistically treated with SPSS13.0 statistics software, and the comparison between the count data groups was statistically analyzed by X2 test. P0.05 was considered as a difference in the correlation between.Livin and XIAP expression using Spearman grade correlation score. Results: the results of 1 HE staining showed that the specimens of HE stained slices were placed under the optical microscope. The results showed that the normal endometrium glands were arranged orderly, the morphology was regular and no mitosis was found; the atypical hyperplasia tissues of the endometrium showed that the cells were heterotypic, the endometrial glands were very disorderly and irregular in permutation, and the endometrium was visible in the uterus. The glandular cells of the adenocarcinoma of the membrane were characterized by an obvious dysplasia, extremely disorderly arrangement and more mitosis. Among them, the adenocarcinoma of the highly differentiated adenocarcinoma was arranged in disorder, crowded, the structure resembles hyperplasia gland, the cell was mild heteromorphic, the adenocarcinoma in the middle differentiated adenocarcinoma was irregular, the cell heteromorphosis was obvious, and the mitosis was easy to see; the low differentiated adenocarcinoma without adenoid structure and nuclear heteromorphosis The positive rate of XIAP expression in endometrioid adenocarcinoma group was 77.5% (31/40), and the positive rate of XIAP expression in the endometrium atypical hyperplasia group was 70% (28/40), and the positive rate of the normal endometrium group was only 20% (8/40). Statistical analysis showed that endometrioid adenocarcinoma group and endometrium were found in endometrioid adenocarcinoma group and endometrium. The positive rate of XIAP expression in the atypical hyperplasia group was significantly higher than that in the normal endometrium group (P0.05). The positive rate of XIAP expression in endometrioid adenocarcinoma group was higher than that of the endometrium atypical hyperplasia group, but the difference was not statistically significant (P0.05) the expression of.3livin protein in the endometrioid adenocarcinoma group was 80% (32/4). 0) the positive rate of Livin expression in the endometrium atypical hyperplasia group was 75% (30/40), and the positive rate of Livin expression in the normal endometrium group was only 22.5% (9/40). The statistical analysis showed that the positive rate of Livin expression in endometrioid adenocarcinoma group and endometrium atypical hyperplasia group was significantly higher than that of normal endometrium group (P0.05), endometrioid adenocarcinoma. The positive rate of Livin expression in the group was higher than that of the endometrium atypical hyperplasia group, but the difference was not statistically significant (P0.05) the relationship between the clinicopathological parameters and the expression of XIAP in the endometrioid adenocarcinoma of.4 was in 40 endometrioid adenocarcinoma tissues. The positive expression of XIAP was associated with the patient's age, the depth of myometrium infiltration, and the metastasis of lymph nodes. The positive rate of XIAP in stage I was 55.6% (5/9), and the positive rate of XIAP in stage II patients was 62.5% (5/8), and the positive rate of XIAP in stage III - IV patients was 91.3% (21/23). Statistical analysis showed that the positive rate of XIAP was statistically different (P0.05) in the clinical stages (P0.05). The positive rate of XIAP at grade G1 was 55.6% (5/9), the positive rate of XIAP at G2 level was 57.1% (4/7), and the positive rate of XIAP in G3 grade was 91.7% (22/24). Statistical analysis showed that the positive rate of XIAP was statistically different in different pathological grades (P0.05) all the clinicopathological parameters and the protein table in the endometrioid adenocarcinoma tissue of the.5 subuterine. In 40 cases of endometrioid adenocarcinoma, the positive expression of Livin was not statistically significant (P0.05). In clinical stage, the positive rate of Livin in stage I was 55.6% (5/9); the positive rate of Livin in stage II patients was 62.5% (5/8), and liv in stage III - IV patients liv. The positive rate of in was 95.7% (22/23). Statistical analysis showed that the positive rate of Livin was statistically different in each clinical stage (P0.05). The positive rate of Livin in G1 grade patients was 55.6% (5/9); the positive rate of Livin at G2 grade was 57.1% (4/7), and G3 class Livin was 95.8%. The expression rate was statistically different in different pathological grades (P0.05) the correlation between XIAP and Livin in.6 endometrioid adenocarcinoma was in XIAP positive endometrioid adenocarcinoma tissue, the positive rate of Livin was 93.5% (29/31). In XIAP negative endometrioid adenocarcinoma tissue, the positive rate of Livin was 33.3% (3/9), and in Livin positive uterus. In endometrioid adenocarcinoma, the positive rate of XIAP was 90.6% (29/32); in Livin negative endometrioid adenocarcinoma tissue, the positive rate of XIAP was 25% (2/8). Statistical analysis showed that the expression of XIAP and Livin in endometrioid adenocarcinoma was correlated, and the two was positively correlated (P0.05). Conclusion: 1 compared with normal endometrium, endometrioid glands are compared. The expression of XIAP and Livin in the atypical hyperplasia of carcinoma and endometrium increased significantly. It was suggested that the overexpression of XIAP and Livin may be associated with the occurrence of endometrioid adenocarcinoma in endometrioid adenocarcinoma. The positive expression of XIAP and Livin is not related to age, lymph node metastasis and depth of infiltration, but with clinical stage and pathology. The higher the clinical stage and pathological grade, the higher the positive expression rate of XIAP and Livin. It is suggested that the overexpression of XIAP and Livin has a certain significance in judging the clinical prognosis of endometrioid adenocarcinoma. In endometrioid adenocarcinoma tissue, the positive expression of Livin is positively correlated with the positive expression of XIAP, and the two have synergistic effect.
【学位授予单位】:河北医科大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:R737.33
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