基于NIR-SERS光谱技术分析宫颈癌氧合血红蛋白

发布时间：2018-08-04 09:55

【摘要】：采用电解法制备新型、高效纳米银膜作为近红外表面增强拉曼散射(NIR-SERS)基底,检测了22例健康女性和22例宫颈癌患者氧合血红蛋白的NIR-SERS光谱。对比宫颈癌患者与健康女性氧合血红蛋白NIR-SERS光谱,发现两者区别明显。利用多变量统计分析方法得到宫颈癌患者与健康女性氧合血红蛋白的NIR-SERS光谱在472、662、720和1209 cm-1谱峰处区别最为明显,诊断的特异性与灵敏度均为86.4%。由谱峰归属分析得知,宫颈癌患者血液中高铁血红蛋白吡咯环折叠振动、氧合血红蛋白吡咯环的对称变形振动、反对称变形振动和吡咯环间CmH基团的变形振动等振动模式与健康女性相比有明显的减少。研究表明,NIR-SERS技术结合多变量统计分析方法可以较好地区分宫颈癌患者和健康女性氧合血红蛋白,有望发展成为一种新型的宫颈癌临床诊断工具。
[Abstract]:A novel and high efficient silver nanocrystalline silver film was prepared by electrolysis as a near-infrared surface-enhanced Raman scattering (NIR-SERS) substrate. The NIR-SERS spectra of oxygenated hemoglobin in 22 healthy women and 22 cervical cancer patients were measured. The NIR-SERS spectra of oxygenated hemoglobin in cervical cancer patients and healthy women were compared. The NIR-SERS spectra of oxygenated hemoglobin in cervical cancer patients and healthy women were found to be the most distinct at the peak of 472662720 and 1209 cm-1 by multivariate statistical analysis. The specificity and sensitivity of diagnosis were both 86.4%. From the spectrum analysis, we know that the blood of cervical cancer patients with methemoglobin pyrrole ring folding vibration, oxygenated hemoglobin pyrrole ring symmetrical deformation vibration, The vibration modes such as antisymmetric deformation vibration and CmH group vibration between pyrrole rings were significantly reduced compared with healthy women. The results show that NIR-SERS technique combined with multivariate statistical analysis can distinguish oxygenated hemoglobin between cervical cancer patients and healthy women, and it is expected to develop into a new clinical diagnosis tool for cervical cancer.
【作者单位】：楚雄师范学院光谱技术应用研究所;遵义医学院基础医学院;
【基金】：国家自然科学基金(11364001,10864001,11064001) 云南省应用基础研究计划青年项目(2013FD044) 楚雄师范学院学术后备人才资助项目(13XJRC19)
【分类号】：O657.37;R737.33

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