子痫前期患者中NFAT5、MCP-1的表达与巨噬细胞浸润的相关性研究
发布时间：2018-08-05 20:07
【摘要】：子痫前期(preeclampsia,PE)是怀孕期间特有的并发症,特点是水肿、高血压、尿蛋白并存,涉及多个器官系统损害。产后上述症状仍持续存在,它是导致全世界母婴病死率增加的主要原因之一,发病率在全球孕妇中为5%~8%。尽管目前针对子痫前期的发病有多种学说,但其发病机制仍不明确。PE分为轻度和重度,重度子痫前期以34周为界分为两个主要亚型,早发型(early-onset severe pre-eclampsia,EPE)与晚发型重度子痫前期(late-onset severe pre-eclampsia,LPE)。EPE的特点为妊娠早中期出现血压及尿蛋白迅速升高,终末器官损害严重,并发症较多,其胎盘功能损伤严重,因此又称为“胎盘源性疾病”,胎儿在宫内生长受限,母婴结局的预后较差,已经引起产科医生高度重视;而LPE的病情发展缓慢,各种临床症状与体征均较EPE轻,胎盘形态学基本正常,多与母体自身疾病相关,又称为“母源性疾病”。核转录因子5(nuclear factor of activated T cells 5,NFAT5)广泛存在于眼睛、肝脏、肾脏、胎盘等多个器官表达。高渗条件下被激活,调节多种渗透性保护基因的表达。组织局部渗透压升高与机体的炎症状态紧密相关。NFAT5在高渗条件下激活后能够促进炎症因子的表达,在自身免疫、细胞应激、炎症性疾病方面发挥着重要的作用。单核细胞趋化蛋白1(monocyte chemoattractant protein-1,MCP-1)含有76个氨基酸,与其受体(chemokine receptor 2,CCR2)结合后趋化或激活炎症细胞聚集到炎症部位,参与机体的病理生理反应。有研究显示滋养细胞和蜕膜细胞可分泌MCP-1,趋化血液中单核巨噬细胞迁移至蜕膜,在螺旋动脉重塑过程中意义重大。最近研究表明在腹膜间皮细胞Met5A细胞中MCP-1启动子区域有Ton E结合位点,NFAT5与其结合后可导致MCP-1表达升高,然而,在子痫前期胎盘组织中NFAT5与MCP-1是否存在相关性国内外未见报道,本研究以此为切入点,探讨子痫前期患者胎盘组织中NFAT5与MCP-1之间的相关性,为子痫前期的发病机制开辟一个新窗口。巨噬细胞能够清除体内细胞凋亡成分,它的免疫调节作用在妊娠期子宫胎盘床附近受到关注。子痫前期患者中有大量的巨噬细胞浸润,通过多种机制调控着滋养细胞的侵袭能力。目前,国外对于巨噬细胞与子痫前期的关系已做了很多研究,但是巨噬细胞浸润和MCP-1、NFAT5的关系国内外研究较少。本研究以此为突破口,通过分析巨噬细胞浸润和MCP-1、NFAT5的关系,初步探讨其在子痫前期发病机制中的作用。目的本研究通过比较MCP-1在不同类型重度子痫前期患者母血、脐血及胎盘组织中的水平,并分析其相关性,为将来早期诊断子痫前期孕妇提供分子标记物和新靶点;通过检测不同类型重度子痫前期胎盘中MCP-1、NFAT5及CD68+巨噬细胞的表达,分析巨噬细胞浸润和MCP-1、NFAT5的关系,初步探讨其在子痫前期发病机制中的作用。方法1研究对象选取在郑州大学第三附属医院产科住院分娩的重度子痫前期患者61例为试验组,其中EPE 31例(早发组),LPE 30例(晚发组),均为剖宫产分娩,收集标本时间为2015年1月至2016年1月。另选取同期正常孕妇30例为对照组,均为健康足月孕妇,因社会因素或骨盆异常等原因行剖宫产。各组孕妇均为单胎妊娠,此次妊娠均为自然受孕,未采取辅助生殖技术;各组孕妇既往均无自身免疫性疾病、糖尿病、染色体结构异常以及胎膜早破等其他妊娠合并症病史。2实验方法1.血清及胎盘标本采集:所有研究对象入院后次日清晨空腹抽取孕妇肘静脉血3ml,脐静脉血则是在胎盘娩出后抽取3ml。室温下放置半小时,随后离心约15min,取上层淡黄色血清分装在EP管中,避免溶血标本,置于-80℃冰箱保存待测。所取的胎盘组织标本均在剖宫产胎盘娩出后15 min内,在母体面中央区域(无出血、梗死及钙化),剪下大小约(1.0 cm×1.0 cm×1.0 cm)2块组织,用生理盐水漂洗后,其中一块投入液氮,后转-80℃冰箱保存备用。另一块固定于福尔马林溶液中,用于免疫组化实验。2.采用酶联免疫吸附(enzyme-linked immunoadsordent assay,ELISA)方法对孕妇母血清、脐血清中MCP-1的水平进行检测。3.采用Real-time PCR技术检测胎盘组织中NFAT5和MCP-1 m RNA的表达水平,数据统计分析采用2-△△CT相对定量方法。4.采用免疫组化方法检测NFAT5、MCP-1、CD68+巨噬细胞在胎盘中的表达。通过图像分析技术对上述三个指标的免疫组化结果进行分析比较。3统计学处理统计学分析采用SPSS 21.0软件。频数资料采用(?)±s表示,多组之间的比较采用单因素方差分析进行,多组之间的两两相比用LSD-t法;用Pearson方法分析两组之间的相关性,检验水准为α=0.05。结果1三组间一般临床资料的比较对照组、早发组和晚发组孕妇之间的年龄、终止孕周、分娩前BMI比较,差异均无统计学意义(P0.05);早发组新生儿出生体重、胎盘重量均低于对照组和晚发组,差异均有统计学意义(P0.05);早发组24h尿蛋白高于晚发组,差异有统计学意义(P0.05)。2三组间母血清、脐血清中MCP-1水平的比较早发组母血清中MCP-1水平185.80±15.73ng/L高于晚发组164.93±6.24ng/L和对照组154.78±9.61ng/L(P0.05);早发组脐血清中MCP-1水平为223.49±41.68ng/L高于晚发组151.85±13.92ng/L和对照组113.91±6.67ng/L(P0.05)。3三组间胎盘中NFAT5和MCP-1 m RNA表达水平的比较早发组胎盘中NFAT5 m RNA的表达水平为(1.07±0.03),高于晚发组(0.92±0.02)及对照组(0.90±0.04)(P0.05);早发组胎盘中MCP-1 m RNA的表达水平为(1.08±0.12)高于晚发组(1.02±0.08)及对照组(0.99±0.02)(P0.05)。4三组间胎盘中NFAT5、MCP-1、CD68+巨噬细胞蛋白水平表达的比较早发组胎盘中NFAT5蛋白的表达水平为(34.16±4.65),高于晚发组(11.39±3.77)及对照组(10.65±1.85)(P0.05);早发组胎盘中MCP-1蛋白的表达水平为(38.47±4.81)高于晚发组(19.29±3.86)及对照组(20.33±3.92)(P0.05);早发组胎盘中CD68+巨噬细胞的表达水平为(80.60±10.47)高于晚发组(24.25±6.69)及对照组(22.79±5.58)(P0.05)。5 NFAT5、MCP-1与CD68+巨噬细胞浸润的相关性分析1.早发组脐血清与母血清中MCP-1呈正相关(r=0.587,P0.05);早发组脐血清与胎盘中MCP-1呈正相关(r=0.754,P0.05);而在晚发组中也均呈正相关(r=0.576,0.791,P均0.05);早发组及晚发组脐血清中MCP-1与新生儿出生体质量均呈负相关(r=-0.508,-0.496,P均0.05)。2.早发组及晚发组母血清中MCP-1水平与胎盘中MCP-1 m RNA的表达水平均呈正相关(r=0.671,0.676,P均0.05)。3.早发组胎盘中NFAT5 m RNA与MCP-1 m RNA表达水平呈正相关(r=0.724,P0.05),早发组胎盘中NFAT5 m RNA表达水平与CD68+巨噬细胞的表达呈正相关(r=0.640,P0.05);而在晚发组中也均呈正相关(r=0.631,0.608,P均0.05)。4.早发组及晚发组胎盘中MCP-1 m RNA表达水平与CD68+巨噬细胞的表达均呈正相关(r=0.704,0.686,P均0.05)。结论1.MCP-1在重度子痫前期孕妇母血、脐血和胎盘中具有相关性,检测其水平为早发型子痫前期的早期诊断提供依据。2.早发型重度子痫前期胎盘中NFAT5、MCP-1、CD68+巨噬细胞的表达两两间呈正相关,说明NFAT5-MCP-1-CD68+巨噬细胞途径可能在早发型重度子痫前期的发病过程中发挥作用。
[Abstract]:Preeclampsia (PE) is a special complication during pregnancy, characterized by edema, high blood pressure, and urinary protein coexistence, involving multiple organ system damage. The postpartum symptoms continue to exist, which is one of the main causes of the increase of maternal and infant mortality in the world, the incidence of 5%~8%. in pregnant women around the world, although the current needle before the eclampsia There are a variety of doctrines in the pathogenesis, but the pathogenesis is still not clearly defined as mild and severe.PE, and severe preeclampsia is divided into two major subtypes with 34 weeks as the boundary. Early onset (early-onset severe pre-eclampsia, EPE) and late onset severe preeclampsia (late-onset severe pre-eclampsia, LPE).EPE are characterized by early and mid trimester blood pressure. And the rapid increase in urine protein, serious end organ damage, more complications and serious injury of the placenta function, so it is also called "placental source disease", the fetus is limited in intrauterine growth, the prognosis of the mother and baby is poor, and the obstetricians have paid much attention to it, and the disease of LPE develops slowly, and all kinds of clinical symptoms and signs are lighter than EPE. The disc morphology is basically normal and is related to the mother's own disease. It is also called "maternal disease". Nuclear transcription factor 5 (nuclear factor of activated T cells 5, NFAT5) is widely expressed in many organs such as eyes, liver, kidney, and placenta. Under hypertonic conditions, it is activated to regulate the expression of various osmotic protective genes. Tissue local osmosis Elevated pressure is closely related to the inflammatory state of the body..NFAT5 can promote the expression of inflammatory factors after activation in hypertonic conditions. It plays an important role in autoimmune, cell stress, and inflammatory diseases. Monocyte chemoattractant protein 1 (monocyte chemoattractant protein-1, MCP-1) contains 76 amino acids, and its receptor (chemokine R). Eceptor 2, CCR2) combine to chemotaxis or activate inflammatory cells to accumulate to the site of inflammation and participate in the body's pathophysiological response. Studies have shown that trophoblast and decidual cells can secrete MCP-1 and migrate to the decidua by mononuclear macrophages in the chemotaxis, which is significant in the process of spiral artery remodeling. Recent studies have shown that Met5 in peritoneal mesothelial cells There is a Ton E binding site in the MCP-1 promoter region of A cells. NFAT5 and its binding can lead to the increase of MCP-1 expression. However, there is no correlation between the existence of NFAT5 and MCP-1 in the placental tissue of preeclampsia. This study is the breakthrough point to explore the correlation between NFAT5 and MCP-1 in placental tissues of preeclampsia patients, which is eclampsia. The early pathogenesis opens up a new window. Macrophages can remove the body's apoptotic components, its immune regulation is concerned near the pregnancy uterus placenta bed. A large number of macrophages infiltrate in preeclampsia, and regulate the invasion ability of the nourishing cell through a variety of mechanisms. A lot of studies have been done on the relationship between cell and preeclampsia, but the relationship between macrophage infiltration and MCP-1, NFAT5 is seldom studied at home and abroad. This study is a breakthrough. By analyzing the relationship between macrophage infiltration and MCP-1 and NFAT5, the role of the macrophage infiltration in the pathogenesis of preeclampsia was preliminarily discussed. The purpose of this study was to compare MCP-1 in different classes. The level of maternal blood, umbilical blood and placenta tissue in patients with severe preeclampsia and their correlation were analyzed to provide molecular markers and new targets for the early diagnosis of preeclampsia. By detecting the expression of MCP-1, NFAT5 and CD68+ macrophages in different types of severe preeclampsia, the infiltration of macrophages and the correlation of MCP-1 and NFAT5 were analyzed. A preliminary study of its role in the pathogenesis of preeclampsia. Method 1 the subjects selected 61 cases of severe preeclampsia in the obstetric department of the Third Affiliated Hospital of Zhengzhou University, 61 cases of severe preeclampsia, of which 31 cases (early onset group) and 30 LPE (late onset group) were Caesarean birth, and the collection time was from January 2015 to January 2016. At the same time, 30 normal pregnant women were selected as the control group, all of which were healthy full term pregnant women. All pregnant women were single pregnancy due to social factors or pelvic abnormality. All pregnant women were single pregnancy. All pregnant women were pregnant with natural pregnancy and did not adopt auxiliary reproductive technology. .2 experimental methods of other pregnancy complications, such as premature rupture of membrane, 1. serum and placenta samples: all the subjects were taken on the next morning after admission to the pregnant women's elbow vein blood 3ml. The umbilical vein blood was placed at the room temperature of 3ml. for half an hour after the delivery of the placenta, and then centrifuged for about 15min, and the upper layer of light yellow sera was separated into the EP tube to avoid dissolving. The specimen of the blood was kept in the refrigerator at -80 C for 15 min after cesarean section. In the central region of the mother body (no bleeding, infarction and calcification), 2 tissues were cut down (1 cm x 1 cm x 1 cm), and one of them was washed with saline, and the other was stored in the refrigerator at -80 centigrade. The block was fixed in the formalin solution and used in the immuno histochemical experiment.2. using enzyme-linked immunoadsordent assay (ELISA) method to detect the level of MCP-1 in maternal maternal serum and umbilical serum..3. was used to detect the expression level of NFAT5 and MCP-1 m in placenta tissue by Real-time PCR technique. Data statistical analysis was adopted. 2- Delta Delta CT relative quantitative method.4. was used to detect the expression of NFAT5, MCP-1, CD68+ macrophages in the placenta by immunohistochemical method. The immunohistochemical results of the above three indexes were analyzed by image analysis technique and compared with.3 statistics processing statistics analysis using SPSS 21 software. Frequency data were expressed by (?) + s, and the ratio of multiple groups was compared. Compared with the single factor analysis of variance, 22 of the multiple groups were compared with the LSD-t method, and the correlation between the two groups was analyzed with the Pearson method. The test level was the comparison group of the general clinical data between the 1 three groups of the alpha =0.05. results, the age, the final gestational age and the BMI comparison between the pregnant women in the early onset group and the late onset group were not statistically significant. P0.05. The birth weight of early onset group was lower than that of the control group and late onset group. The difference was statistically significant (P0.05). The 24h urine protein in the early onset group was higher than that in the late onset group (P0.05), the difference was statistically significant (P0.05) of the mother serum of.2 three groups, and the level of MCP-1 in the umbilical serum was compared to the level of MCP-1 in the maternal serum of the early onset group, and the level of MCP-1 was higher than that in the late onset group. The group 164.93 + 6.24ng/L and the control group were 154.78 + 9.61ng/L (P0.05), and the level of MCP-1 in the umbilical serum in the early onset group was 223.49 + 41.68ng/L higher than that in the late onset group 151.85 + 13.92ng/L and the control group was 113.91 + 6.67ng/L (P0.05).3 three. The expression level of NFAT5 and MCP-1 m in the placenta was (1.07 + 0.03). Higher than the late onset group (0.92 + 0.02) and the control group (0.90 + 0.04) (P0.05), the expression level of MCP-1 m RNA in the placenta of the early onset group was higher than that in the late onset group (1.02 + 0.08) and the expression of NFAT5, MCP-1, and CD68+ macrophage protein in the placenta of the control group (0.99 + 0.02) (0.99 + 0.02) (P0.05).4 three. The expression level of NFAT5 protein in the placenta of the early onset group (34.16 + 4.65), higher than that in the late onset group (11.39 + 3.77) and the control group (10.65 + 1.85) (P0.05), the expression level of MCP-1 protein in the placenta of the early onset group was higher than that in the late onset group (19.29 + 3.86) and the control group (20.33 + 3.92) (P0.05), and the expression level of the placenta in the early onset group was higher than that of the late onset group. The correlation between (22.79 + 5.58) (22.79 + 5.58) (P0.05).5 NFAT5, MCP-1 and CD68+ macrophage infiltration in the early onset group, the umbilical serum was positively correlated with the MCP-1 in the maternal serum (r=0.587, P0.05); the umbilical serum in the early onset group was positively correlated with the MCP-1 in the placenta (r=0.754, P0.05), but also in the late onset group (r=0.576,0.791, 0.05), and the early onset and late onset groups There was a negative correlation between MCP-1 in umbilical serum and the quality of newborn birth body (r=-0.508, -0.496, P 0.05). The level of MCP-1 in the early hair group and the maternal serum of the late onset group was positively correlated with the expression level of MCP-1 m RNA in the placenta (r=0.671,0.676, P are 0.05). The expression level of NFAT5 m RNA in the placenta of hair group was positively correlated with the expression of CD68+ macrophage (r=0.640, P0.05), but also in the late onset group (r=0.631,0.608, P all 0.05).4. early onset group and the expression level of MCP-1 m RNA in the late hair group was positively correlated with the expression of macrophage. The correlation between maternal blood, umbilical cord blood and placenta in preeclampsia pregnant women was found to provide a basis for early diagnosis of early onset preeclampsia to provide a basis for 22 positive correlations between the expression of NFAT5, MCP-1, and CD68+ macrophages in the placenta of early onset severe preeclampsia, indicating that the NFAT5-MCP-1-CD68+ macrophage pathway may be in early onset severe eclampsia. It plays a role in the process of early onset.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244
[Abstract]:Preeclampsia (PE) is a special complication during pregnancy, characterized by edema, high blood pressure, and urinary protein coexistence, involving multiple organ system damage. The postpartum symptoms continue to exist, which is one of the main causes of the increase of maternal and infant mortality in the world, the incidence of 5%~8%. in pregnant women around the world, although the current needle before the eclampsia There are a variety of doctrines in the pathogenesis, but the pathogenesis is still not clearly defined as mild and severe.PE, and severe preeclampsia is divided into two major subtypes with 34 weeks as the boundary. Early onset (early-onset severe pre-eclampsia, EPE) and late onset severe preeclampsia (late-onset severe pre-eclampsia, LPE).EPE are characterized by early and mid trimester blood pressure. And the rapid increase in urine protein, serious end organ damage, more complications and serious injury of the placenta function, so it is also called "placental source disease", the fetus is limited in intrauterine growth, the prognosis of the mother and baby is poor, and the obstetricians have paid much attention to it, and the disease of LPE develops slowly, and all kinds of clinical symptoms and signs are lighter than EPE. The disc morphology is basically normal and is related to the mother's own disease. It is also called "maternal disease". Nuclear transcription factor 5 (nuclear factor of activated T cells 5, NFAT5) is widely expressed in many organs such as eyes, liver, kidney, and placenta. Under hypertonic conditions, it is activated to regulate the expression of various osmotic protective genes. Tissue local osmosis Elevated pressure is closely related to the inflammatory state of the body..NFAT5 can promote the expression of inflammatory factors after activation in hypertonic conditions. It plays an important role in autoimmune, cell stress, and inflammatory diseases. Monocyte chemoattractant protein 1 (monocyte chemoattractant protein-1, MCP-1) contains 76 amino acids, and its receptor (chemokine R). Eceptor 2, CCR2) combine to chemotaxis or activate inflammatory cells to accumulate to the site of inflammation and participate in the body's pathophysiological response. Studies have shown that trophoblast and decidual cells can secrete MCP-1 and migrate to the decidua by mononuclear macrophages in the chemotaxis, which is significant in the process of spiral artery remodeling. Recent studies have shown that Met5 in peritoneal mesothelial cells There is a Ton E binding site in the MCP-1 promoter region of A cells. NFAT5 and its binding can lead to the increase of MCP-1 expression. However, there is no correlation between the existence of NFAT5 and MCP-1 in the placental tissue of preeclampsia. This study is the breakthrough point to explore the correlation between NFAT5 and MCP-1 in placental tissues of preeclampsia patients, which is eclampsia. The early pathogenesis opens up a new window. Macrophages can remove the body's apoptotic components, its immune regulation is concerned near the pregnancy uterus placenta bed. A large number of macrophages infiltrate in preeclampsia, and regulate the invasion ability of the nourishing cell through a variety of mechanisms. A lot of studies have been done on the relationship between cell and preeclampsia, but the relationship between macrophage infiltration and MCP-1, NFAT5 is seldom studied at home and abroad. This study is a breakthrough. By analyzing the relationship between macrophage infiltration and MCP-1 and NFAT5, the role of the macrophage infiltration in the pathogenesis of preeclampsia was preliminarily discussed. The purpose of this study was to compare MCP-1 in different classes. The level of maternal blood, umbilical blood and placenta tissue in patients with severe preeclampsia and their correlation were analyzed to provide molecular markers and new targets for the early diagnosis of preeclampsia. By detecting the expression of MCP-1, NFAT5 and CD68+ macrophages in different types of severe preeclampsia, the infiltration of macrophages and the correlation of MCP-1 and NFAT5 were analyzed. A preliminary study of its role in the pathogenesis of preeclampsia. Method 1 the subjects selected 61 cases of severe preeclampsia in the obstetric department of the Third Affiliated Hospital of Zhengzhou University, 61 cases of severe preeclampsia, of which 31 cases (early onset group) and 30 LPE (late onset group) were Caesarean birth, and the collection time was from January 2015 to January 2016. At the same time, 30 normal pregnant women were selected as the control group, all of which were healthy full term pregnant women. All pregnant women were single pregnancy due to social factors or pelvic abnormality. All pregnant women were single pregnancy. All pregnant women were pregnant with natural pregnancy and did not adopt auxiliary reproductive technology. .2 experimental methods of other pregnancy complications, such as premature rupture of membrane, 1. serum and placenta samples: all the subjects were taken on the next morning after admission to the pregnant women's elbow vein blood 3ml. The umbilical vein blood was placed at the room temperature of 3ml. for half an hour after the delivery of the placenta, and then centrifuged for about 15min, and the upper layer of light yellow sera was separated into the EP tube to avoid dissolving. The specimen of the blood was kept in the refrigerator at -80 C for 15 min after cesarean section. In the central region of the mother body (no bleeding, infarction and calcification), 2 tissues were cut down (1 cm x 1 cm x 1 cm), and one of them was washed with saline, and the other was stored in the refrigerator at -80 centigrade. The block was fixed in the formalin solution and used in the immuno histochemical experiment.2. using enzyme-linked immunoadsordent assay (ELISA) method to detect the level of MCP-1 in maternal maternal serum and umbilical serum..3. was used to detect the expression level of NFAT5 and MCP-1 m in placenta tissue by Real-time PCR technique. Data statistical analysis was adopted. 2- Delta Delta CT relative quantitative method.4. was used to detect the expression of NFAT5, MCP-1, CD68+ macrophages in the placenta by immunohistochemical method. The immunohistochemical results of the above three indexes were analyzed by image analysis technique and compared with.3 statistics processing statistics analysis using SPSS 21 software. Frequency data were expressed by (?) + s, and the ratio of multiple groups was compared. Compared with the single factor analysis of variance, 22 of the multiple groups were compared with the LSD-t method, and the correlation between the two groups was analyzed with the Pearson method. The test level was the comparison group of the general clinical data between the 1 three groups of the alpha =0.05. results, the age, the final gestational age and the BMI comparison between the pregnant women in the early onset group and the late onset group were not statistically significant. P0.05. The birth weight of early onset group was lower than that of the control group and late onset group. The difference was statistically significant (P0.05). The 24h urine protein in the early onset group was higher than that in the late onset group (P0.05), the difference was statistically significant (P0.05) of the mother serum of.2 three groups, and the level of MCP-1 in the umbilical serum was compared to the level of MCP-1 in the maternal serum of the early onset group, and the level of MCP-1 was higher than that in the late onset group. The group 164.93 + 6.24ng/L and the control group were 154.78 + 9.61ng/L (P0.05), and the level of MCP-1 in the umbilical serum in the early onset group was 223.49 + 41.68ng/L higher than that in the late onset group 151.85 + 13.92ng/L and the control group was 113.91 + 6.67ng/L (P0.05).3 three. The expression level of NFAT5 and MCP-1 m in the placenta was (1.07 + 0.03). Higher than the late onset group (0.92 + 0.02) and the control group (0.90 + 0.04) (P0.05), the expression level of MCP-1 m RNA in the placenta of the early onset group was higher than that in the late onset group (1.02 + 0.08) and the expression of NFAT5, MCP-1, and CD68+ macrophage protein in the placenta of the control group (0.99 + 0.02) (0.99 + 0.02) (P0.05).4 three. The expression level of NFAT5 protein in the placenta of the early onset group (34.16 + 4.65), higher than that in the late onset group (11.39 + 3.77) and the control group (10.65 + 1.85) (P0.05), the expression level of MCP-1 protein in the placenta of the early onset group was higher than that in the late onset group (19.29 + 3.86) and the control group (20.33 + 3.92) (P0.05), and the expression level of the placenta in the early onset group was higher than that of the late onset group. The correlation between (22.79 + 5.58) (22.79 + 5.58) (P0.05).5 NFAT5, MCP-1 and CD68+ macrophage infiltration in the early onset group, the umbilical serum was positively correlated with the MCP-1 in the maternal serum (r=0.587, P0.05); the umbilical serum in the early onset group was positively correlated with the MCP-1 in the placenta (r=0.754, P0.05), but also in the late onset group (r=0.576,0.791, 0.05), and the early onset and late onset groups There was a negative correlation between MCP-1 in umbilical serum and the quality of newborn birth body (r=-0.508, -0.496, P 0.05). The level of MCP-1 in the early hair group and the maternal serum of the late onset group was positively correlated with the expression level of MCP-1 m RNA in the placenta (r=0.671,0.676, P are 0.05). The expression level of NFAT5 m RNA in the placenta of hair group was positively correlated with the expression of CD68+ macrophage (r=0.640, P0.05), but also in the late onset group (r=0.631,0.608, P all 0.05).4. early onset group and the expression level of MCP-1 m RNA in the late hair group was positively correlated with the expression of macrophage. The correlation between maternal blood, umbilical cord blood and placenta in preeclampsia pregnant women was found to provide a basis for early diagnosis of early onset preeclampsia to provide a basis for 22 positive correlations between the expression of NFAT5, MCP-1, and CD68+ macrophages in the placenta of early onset severe preeclampsia, indicating that the NFAT5-MCP-1-CD68+ macrophage pathway may be in early onset severe eclampsia. It plays a role in the process of early onset.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244
【相似文献】
相关期刊论文 前10条
1 宋成文;王泽华;;子痫前期患者的肾素-血管紧张素基因多态性研究进展[J];实用医学杂志;2006年14期
2 刘全;贺晶;董岳;胡文胜;王焓知;;子痫前期患者血清巨噬细胞集落刺激因子增高的意义[J];浙江大学学报(医学版);2005年06期
3 李桦;马玉燕;牟瑞丽;王磊一;;血管紧张素转化酶基因和血管紧张素Ⅱ受体1基因多态性与子痫前期患者蛋白尿的相关性研究[J];中华妇产科杂志;2006年04期
4 王伽略;杨孜;王荣;李智;李惠娟;曾琳;;子痫前期患者血脂代谢调节的探讨[J];现代妇产科进展;2006年06期
5 齐向红;范书萍;秦英;冯静;李季滨;吴化平;李丽;;子痫前期患者血清神经激肽B含量测定及其临床意义[J];河北医药;2006年11期
6 卢芝君;;重度子痫前期患者血清胱抑素C与尿酸测定的临床意义[J];江西医学检验;2007年02期
7 齐向红;范书萍;秦英;冯静;李季滨;吴化平;李丽;;子痫前期患者血清神经激肽B含量测定及其临床意义[J];中国妇幼保健;2007年14期
8 吴媛媛;乔福元;;瘦素及脂联素在子痫前期患者血清中的表达及相关性研究[J];华中医学杂志;2007年06期
9 古旭东;陈建波;;血清胱抑素C与尿转化生长因子β_1在重度子痫前期患者中的临床意义[J];国际检验医学杂志;2008年08期
10 李晓红;杨艳瑞;常雅丽;;子痫前期患者血清中脂联素与血脂水平的变化[J];河北医药;2008年06期
相关会议论文 前10条
1 张纪;古航;崔英;;子痫前期患者脐血中内源性硫化氢浓度变化的研究[A];第四届长三角妇产科学术论坛暨浙江省2009年妇产科学术年会论文汇编[C];2009年
2 刘全;贺晶;董e，
本文编号：2166878
本文链接：https://www.wllwen.com/yixuelunwen/fuchankeerkelunwen/2166878.html
最近更新
教材专著
