卵巢功能调控相关蛋白在不同发育阶段卵泡中的表达及其意义

发布时间：2018-08-07 15:39

【摘要】：目的:检测卵巢功能调控相关蛋白在不同发育阶段卵泡中的表达,探讨其在卵泡发育及卵巢功能调控中的作用及其意义。方法:选取包含原始卵泡、初级卵泡、次级卵泡、成熟卵泡、闭锁卵泡及白体的育龄期女性卵巢组织,采用免疫组织化学法分析乙二醛酶I(Glyoxalase I)、泛素羧基末端水解酶L1(UCH-L1)、热休克蛋白27(HSP27)和血清淀粉样蛋白P(SAP)在不同阶段卵泡中的表达情况,并比较卵泡中其表达强度。结果:Glyoxalas I和UCH-L1在次级卵泡、成熟卵泡的颗粒细胞及卵泡膜细胞中呈强表达,表达强度高于其在原始卵泡及初级卵泡胞质中的表达;HSP27在原始卵泡及初级卵泡细胞质中呈强表达,表达强度高于其在在次级卵泡和成熟卵泡颗粒细胞及卵泡膜细胞中的表达;Glyoxalase I和HSP27在闭锁卵泡中呈强表达,表达强度高于其在生长卵泡中的表达;SAP在原始卵泡、初级卵泡、次级卵泡和成熟卵泡均表达,表达强度无明显差异;4种蛋白在白体中均不表达。结论:UCH-L1强表达和HSP27弱表达与卵泡成熟发育有关;Glyoxalase I强表达及HSP27强表达与卵泡闭锁及卵巢功能衰退有关。
[Abstract]:Aim: to detect the expression of ovarian functional regulation related protein in follicles at different stages of development and to explore its role and significance in follicular development and ovarian function regulation. Methods: the ovarian tissues of women with primary follicles, secondary follicles, mature follicles, atresia follicles and white bodies were selected. The expressions of Glyoxal enzyme I (Glyoxalase I), ubiquitin terminal hydrolase L1 (UCH-L1), heat shock protein 27 (HSP27) and serum amyloid P (SAP) in different stages of follicles were analyzed by immunohistochemical method. Results the expression of glioxalas I and UCH-L1 in secondary follicles, granulosa cells and theca cells of mature follicles was stronger than that in primordial and primary follicular cytoplasm. HSP27 was strongly expressed in the cytoplasm of primordial follicles and primary follicles. The expression intensity of Glyoxalase I and HSP27 in atretic follicles was higher than that in secondary follicles and mature follicle granulosa cells and theca cells. The expression intensity of Glyoxalase I and HSP27 in atretic follicles was higher than that in the growth follicles. The expression intensity of Glyoxalase I and HSP27 in primary follicles and primary follicles was higher than that in growth follicles. Secondary follicles and mature follicles were expressed, and no significant difference in expression intensity was found among the four proteins. Conclusion the strong expression of HSP27 and the strong expression of HSP27 are related to the strong expression of Glyoxalase I and the strong expression of HSP27 to follicular atresia and ovarian dysfunction.
【作者单位】：吉林大学第一医院妇产科;
【基金】：吉林省科技厅自然科学基金资助课题(20160101072JC)
【分类号】：R71

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