12种microRNA在卵巢癌中的表达及临床意义

发布时间：2018-08-12 18:36

【摘要】：目的利用公共数据库分析卵巢癌组织中12种micro RNA(mi R)的表达及其临床意义。方法筛选在GSE14407数据集和TCGA数据库中均具有表达信息的micro RNA,得到mi R-10B、mi R-1244、mi R-622、mi R-21、mi R-503、Let-7D、mi R-155、mi R-30C、mi R-17、mi R-101-1、mi R-186以及mi R-770共12种micro RNA,在这12种micro RNA中寻找在卵巢癌(卵巢癌组)和癌旁组织(癌旁组)中表达有差异的基因。利用在TCGA数据库中筛选得到的505例卵巢癌患者数据,年龄按照中位数分为高年龄(≥59岁)组和低年龄(59岁)组;临床分期分为早期组(Ⅰ期+Ⅱ期)、晚期组(Ⅲ期+Ⅳ期)及未知;肿瘤分级分为低级别(G1+G2)组和高级别(G3+G4)组;病发位置分为单侧组、双侧组;肿瘤残余分为10 mm组、≥10 mm组;micro RNA按照表达量的中位数分为高表达组和低表达组,进行Kaplan-Meier单因素生存分析和多因素Cox回归分析。结果癌旁组Let-7D和mi R-101-1表达水平高于卵巢癌组,而mi R-155和mi R-770表达水平低于卵巢癌组(均P0.05)。生存分析显示,年龄≥59岁、临床分期(Ⅲ+Ⅳ)期和Let-7D低表达的患者生存情况较差(P0.05)。Let-7D低表达是卵巢癌患者生存的独立危险因素。结论 Let-7D的表达与卵巢癌预后相关,可能是卵巢癌预后的独立影响指标。
[Abstract]:Objective to analyze the expression of 12 kinds of micro RNA (mi R) and its clinical significance in ovarian cancer by using public database. Methods micro RNAs expressing information in GSE14407 data set and TCGA database were screened, and 12 micro RNAs were obtained in the 12 micro RNA types: mi R-10BPU mi R-1244N mi R-622mmi R-222mmi R-503n R-503n R-155Cnmi R-30Cnmi R-17Cmmi R-101-1mmi R-186 and mi R-770 micro RNAs. Among the 12 micro RNA RNAs, 12 micro RNAs were found in ovarian cancer (ovarian cancer group) and paracarcinomatous group. Differentially expressed genes are expressed in the tissue (paracancerous group). According to the data of 505 ovarian cancer patients screened in TCGA database, age was divided into high age group (鈮，

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