金雀异黄素对PCOS大鼠卵巢和子宫组织中P450arom mRNA的作用

发布时间：2018-08-16 12:51

【摘要】：目的:通过建立多囊卵巢综合征(PCOS)高雄血症动物模型,观察金雀异黄素(GEN)对大鼠卵巢组织中雌雄激素转换关键酶P450芳香化酶(P450arom)的影响。方法:按照胰岛素(INS)联合HCG造模法建立实验动物模型,分为阴性对照组、模型组、GEN低、中、高剂量组及雌激素阳性对照组,其中剂量组分别灌胃GEN 5,10,20 mg/(kg体质量),雌激素组给予己烯雌酚0.5 mg/kg,持续15 d,观察动情周期10 d。实验结束后,取大鼠血液、子宫和卵巢,ELISA方法测定大鼠血清中雌二醇(E2)、睾酮(T)水平,并计算E2/T比值。RT-PCR法测定大鼠子宫和卵巢中P450arom m RNA表达情况。结果:与模型组相比,中、高剂量组及雌激素组大鼠血清E2水平上升,差异显著(P0.05);各GEN剂量组大鼠血清睾酮水平下降,差异不显著(P0.05);各GEN剂量组及雌激素组E2/T比值升高,差异显著(P0.05)。与模型组相比,高剂量组大鼠卵巢和子宫中P450arom m RNA水平下降明显(P0.05),差异显著;中、低剂量组大鼠卵巢和子宫中P450arom m RNA水平也呈下降趋势,差异不显著(P0.05)。结论 :不同剂量GEN可通过升高大鼠血清E2/T比值,拟补芳香化酶芳香化的缺陷;高剂量【20 mg/(kg体质量)】金雀异黄素可使PCOS大鼠卵巢和子宫组织中P450arom m RNA表达量显著升高,其效果与雌激素相近。
[Abstract]:Objective: To observe the effect of genistein (GEN) on the key enzyme of estrogen and androgen conversion (P450arom) in ovarian tissues of rats by establishing hyperandrogenism animal model of polycystic ovary syndrome (PCOS). Methods: According to insulin (INS) combined with HCG method, the experimental animal model was established and divided into negative control group, model group with low GEN and middle GEN. The estrogen group was given diethylstilbestrol 0.5 mg/kg for 15 days, and the estrous cycle was observed for 10 days. After the experiment, the blood, uterus and ovary of rats were taken out. The levels of estradiol (E2) and testosterone (T) in serum were measured by ELISA, and the E2/T ratio was calculated. Results: Compared with the model group, the levels of serum E2 in the middle and high dose group and estrogen group increased significantly (P 0.05); the levels of serum testosterone in each GEN dose group decreased, the difference was not significant (P 0.05); the E2/T ratio in each GEN dose group and estrogen group increased significantly (P 0.05). (P 0.05). Compared with the model group, the level of P450 AROM m RNA in ovary and uterus of rats in high dose group decreased significantly (P 0.05), the difference was significant; the level of P450 AROM m RNA in ovary and uterus of rats in middle and low dose group also showed a downward trend, the difference was not significant (P 0.05). Conclusion: Different doses of GEN can increase the ratio of E2/T in serum of rats to supplement aromatase. High dose of genistein (20 mg / (kg body weight)) could significantly increase the expression of P450arom m RNA in ovaries and uterus of PCOS rats, and the effect was similar to that of estrogen.
【作者单位】：黑龙江八一农垦大学;哈尔滨医科大学;
【基金】：黑龙江省博士后科研启动基金(LBH-Q15112) 国家自然科学基金-青年基金项目(81102136) 黑龙江省高校农产品加工与质量安全创新团队课题(2014TD006)
【分类号】：R711.75

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