葛根素对妊娠期糖尿病大鼠胰腺组织let-7f及免疫因子IL-17、IL-23表达的影响

发布时间：2018-08-23 15:51

【摘要】：目的:探究葛根素对妊娠期糖尿病大鼠胰腺组织let-7f及免疫因子IL-17、IL-23表达的影响。方法:将120只大鼠分为正常对照组、空白对照组、辛伐他汀组、葛根素低剂量组、葛根素中剂量组、葛根素高剂量组,正常对照组以基础饲料、其他组以高脂饲料饲喂2周。辛伐他汀组给予2 mg/kg的辛伐他汀,葛根素组按低、中、高剂量分别给予70 mg/kg、120mg/kg、170 mg/kg葛根素,正常对照组和空白对照组给予等量生理盐水,治疗2周后,测定6组大鼠血糖和血脂水平、胰腺组织中let-7f的表达水平以及血清和胰腺组织中IL-17和IL-23的表达情况。结果:治疗后,辛伐他汀组和葛根素组大鼠的FBG、TC、TG、FFA显著低于空白对照组,且随着葛根素剂量的增加,FBG、TC、TG、FFA的水平显著降低(P0.05),呈剂量依赖性;辛伐他汀组和葛根素组胰腺组织中let-7f的表达显著高于空白对照组(P0.05),且呈剂量依赖性;辛伐他汀组和葛根素组血清和胰腺组织中IL-17和IL-23的表达显著高于空白对照组(P0.05),且呈剂量依赖性。结论:葛根素能提高妊娠期糖尿病大鼠胰腺组织let-7f表达,同时降低血清和胰腺组织中IL-17、IL-23表达,为妊娠期糖尿病临床治疗提供新的思路和理论依据。
[Abstract]:Aim: to investigate the effects of puerarin on the expression of let-7f and IL-17 IL-23 in pancreatic tissue of gestational diabetic rats. Methods: 120 rats were divided into normal control group, blank control group, simvastatin group, puerarin low dose group, puerarin medium dose group, puerarin high dose group, the normal control group was fed with basic diet, the other groups were fed with high fat diet for 2 weeks. Simvastatin group was given simvastatin for 2 mg/kg, puerarin group was given 70 mg / kg 120 mg / kg puerarin at low, middle and high doses, and normal control group and blank control group were given the same amount of normal saline after 2 weeks of treatment. The levels of blood glucose and blood lipid, the expression of let-7f in pancreatic tissue and the expression of IL-17 and IL-23 in serum and pancreatic tissue were measured in 6 groups. Results: after treatment, the FFA levels in simvastatin group and puerarin group were significantly lower than those in control group. The expression of let-7f in pancreatic tissue of simvastatin group and puerarin group was significantly higher than that of blank control group (P0.05), and the expression was dose-dependent. The expression of IL-17 and IL-23 in serum and pancreas in simvastatin group and puerarin group were significantly higher than those in control group (P0.05). Conclusion: puerarin can increase the expression of let-7f in pancreatic tissue of gestational diabetic rats and decrease the expression of IL-17 IL-23 in serum and pancreatic tissue, which provides a new idea and theoretical basis for the clinical treatment of gestational diabetes mellitus.
【作者单位】：昆明医科大学第一附属医院;
【基金】：国家自然科学基金地区项目(81460240) 云南省科技厅-昆明医科大学应用基础研究联合专项资金项目青年博士项目[2017FE467(-140)] 云南省卫生和计划生育委员会医学后备人才培养计划(H-201646)
【分类号】：R714.256

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