Dicer1在宫颈肿瘤组织中的表达及意义

发布时间：2018-08-24 17:14

【摘要】：目的:探讨Dicer1在正常宫颈上皮、宫颈上皮内瘤变和宫颈鳞癌的表达及意义。方法:Western blot和RT-PCR法检测正常细胞系(End1/E6E7)、宫颈内瘤变细胞系(S12)及宫颈癌细胞系(Ca Ski、He La、Si Ha和C33A)中Dicer1蛋白及其mRNA表达水平,免疫组织化学技术检测Dicer1在正常宫颈、宫颈上皮内瘤变和宫颈鳞癌中的表达水平。结果:正常宫颈上皮细胞系Dicer1表达水平低于宫颈癌细胞系表达量。随着恶性程度增加,Dicer1呈驼峰状表达,即在正常宫颈组织及Grade III级宫颈鳞癌中表达较低,但在宫颈上皮内瘤变及高分化宫颈肿瘤(grade I级)中表达较高。结论:Dicer1在正常宫颈、宫颈上皮内瘤变和宫颈鳞癌中的表达有差异,为进一步研究Dicer1在宫颈癌发生发展中的作用及分子机制提供依据。
[Abstract]:Objective: to investigate the expression and significance of Dicer1 in normal cervical epithelium, cervical intraepithelial neoplasia and cervical squamous cell carcinoma. Methods the expression of Dicer1 protein and mRNA in normal cell line (End1/E6E7), cervical carcinoma cell line (S12) and cervical carcinoma cell line (Ca Ski,He La,Si Ha and C33A) were detected by Western blot and RT-PCR. The expression of Dicer1 in normal cervix was detected by immunohistochemistry. Expression level of cervical intraepithelial neoplasia and cervical squamous cell carcinoma. Results: the expression level of Dicer1 in normal cervical epithelial cell line was lower than that in cervical carcinoma cell line. The expression of Dicer1 in normal cervix and Grade III grade cervical squamous cell carcinoma was lower with the increase of malignant degree, but was higher in (grade I grade of cervical intraepithelial neoplasia and highly differentiated cervical carcinoma. Conclusion there are differences in the expression of Dicer1 in normal cervix, cervical intraepithelial neoplasia and cervical squamous cell carcinoma, which provides a basis for further study of the role and molecular mechanism of Dicer1 in the carcinogenesis and development of cervical cancer.
【作者单位】：华中科技大学同济医学院附属武汉儿童医院;华中科技大学同济医学院附属同济医院;
【基金】：国家自然基金项目(No:81472444)
【分类号】：R737.33

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