T细胞亚群与子痫前期的相关性研究
发布时间：2018-08-26 14:09
【摘要】：目的:子痫前期(preeclampsia PE)是一类以全身小血管痉挛,炎症反应及肾脏损伤为主要特点的妊娠期特有的并发症,初步研究T细胞亚群是否和子痫前期之间的相关性及不同比例的T细胞亚群对子痫前期患者的肾功及血脂的影响。方法:对于2016年7月-2017年1月期间在大连医科大学附属第一医院产科住院的孕妇分组,实验组子痫前期重度孕妇24例(A组)、子痫前期轻度孕妇21例(B组)及对照组正常妊娠孕妇24例(C组)的临床资料进行分析,流式细胞术检验所研究的孕妇T细胞亚群的比例,判断子痫前期与T细胞亚群的相关性。采用SPSS19.0对研究数据进行单因素方差及Pearson相关系数检验。结果:1、子痫前期重度组(A组)、子痫前期轻度组(B组)、正常妊娠组(C组)相比较,患者的年龄、孕次的均值水平差异不显著,组间差异也无统计学意义(均P0.05);子痫前期重度组及子痫前期轻度组的孕龄均值水平与正常妊娠组比较,差异均具有显著的统计学意义(P0.05),而子痫前期重度组及子痫前期轻度组之间的差异无统计学意义(P0.05)。2、通过流式细胞术的检测,发现子痫前期重度患者CD4+T细胞数(TH)与子痫前期轻度组及正常妊娠组相比,显著升高(P0.05),而CD8+T细胞数(TS)明显降低,显著低于正常妊娠组均值水平(P0.05),因而CD4+细胞数/CD8+细胞数比值(TH/TS),子痫前期重度组与子痫前期轻度组及正常妊娠组相比,明显升高(P0.05),同时研究发现子痫前期重度患者的TH/TS水平显著高于子痫前期轻度患者的均值水平(P0.05)。3、尿酸水平:子痫前期重度组与子痫前期轻度组、正常妊娠组相比,尿酸均值水平显著高于正常妊娠组(P0.05),子痫前期轻度组与正常妊娠组相比较,尿酸均值水平相差不大(P0.05);肾小球滤过率(GFR):子痫前期重度组相较于子痫前期轻度组和正常妊娠组而言,GFR均值水平明显降低(P0.05),而子痫前期轻度组与正常妊娠组之间GFR水平差异不具有统计学意义(P0.05);总胆固醇水平:子痫前期重度组、正常妊娠组及子痫前期轻度组比较,前两者稍高于后者(P0.05),同时前两组也高于正常范围;子痫前期重度组与正常妊娠组之间无显著性差异(P0.05);甘油三酯水平:三组均值均明显高于正常范围,子痫前期重度组、子痫前期轻度组与正常妊娠组(对照组)相比,该指标前两组明显高于正常妊娠组(P0.05),而子痫前期重度组该指标均值水平与子痫前期轻度组差异不具有统计学意义(P0.05)。子痫前期重度患者的尿酸、甘油三酯、总胆固醇水平显著提高,而GFR则显著下降。4、子痫前期患者血液中尿酸水平与TH具有正相关关系,相关系数为0.752(P0.05);GFR与TH/TS具有负相关关系,相关系数为-0.601(P0.05);总胆固醇与TH具有正相关性(P0.05),相关系数为0.707;甘油三酯与TH具有正相关关系,相关系数为0.590(P0.05),说明T细胞亚群与肾脏损伤及血脂异常紧密相关。结论:1、子痫前期与T细胞亚群具有相关性,子痫前期重度患者TH、TH/TS显著升高,TS显著下降。2、子痫前期重度患者的尿酸、总胆固醇、甘油三酯水平显著升高,肾小球滤过率明显下降。3、相关性分析表明,子痫前期重度患者的尿酸、肾小球滤过率、总胆固醇及甘油三酯水平均与T细胞亚群具有相关性。
[Abstract]:Objective: Preeclampsia PE is a kind of pregnancy-specific complications characterized by systemic vasospasm, inflammation and kidney injury. To study the correlation between T cell subsets and preeclampsia and the effect of different proportion of T cell subsets on renal function and blood lipids in preeclampsia patients. From July 2016 to January 2017, the clinical data of 24 pregnant women with severe preeclampsia (Group A), 21 mild preeclampsia (Group B) and 24 normal pregnant women (Group C) were analyzed. The T lymphocyte subsets of pregnant women were detected by flow cytometry. SPSS19.0 was used to test the single factor variance and Pearson correlation coefficient. Results: 1. Compared with severe preeclampsia group (group A), mild preeclampsia group (group B) and normal pregnancy group (group C), there was no significant difference in the mean level of age and pregnancy between groups. There was no significant difference between severe preeclampsia group and mild preeclampsia group (P 0.05). The mean gestational age of severe preeclampsia group and mild preeclampsia group had significant statistical significance (P 0.05). However, there was no significant difference between severe preeclampsia group and mild preeclampsia group (P 0.05). Compared with mild preeclampsia group and normal pregnancy group, the number of CD4 + T cells (TH) in severe preeclampsia group was significantly higher (P 0.05), while the number of CD8 + T cells (TS) was significantly lower than the mean level of normal pregnancy group (P 0.05), so the ratio of CD4 + cell number to CD8 + cell number (TH / TS), severe preeclampsia group and mild preeclampsia group and normal pregnancy was significantly lower. The levels of TH / TS and uric acid in severe preeclampsia group were significantly higher than those in mild preeclampsia group (P 0.05). The levels of uric acid in severe preeclampsia group were significantly higher than those in mild preeclampsia group and normal preeclampsia group (P 0.05). Compared with normal pregnancy group, the mean level of uric acid was not significantly different (P 0.05); glomerular filtration rate (GFR): Compared with mild preeclampsia group and normal pregnancy group, the mean level of GFR in severe preeclampsia group was significantly lower (P 0.05), but there was no significant difference between mild preeclampsia group and normal pregnancy group (P 0.0). 5) Total cholesterol level: compared with severe preeclampsia group, normal pregnancy group and mild preeclampsia group, the former two groups were slightly higher than the latter (P 0.05), and the former two groups were also higher than the normal range; there was no significant difference between severe preeclampsia group and normal pregnancy group (P 0.05); triglyceride level: the mean values of the three groups were significantly higher than the normal range, preeclampsia group were significantly higher than the normal range. In severe preeclampsia group, the levels of uric acid, triglyceride and total cholesterol in severe preeclampsia group were not significantly different from those in mild preeclampsia group (P 0.05). The serum uric acid level was positively correlated with TH, and the correlation coefficient was 0.752 (P 0.05); GFR was negatively correlated with TH / TS, and the correlation coefficient was - 0.601 (P 0.05); total cholesterol was positively correlated with TH (P 0.05), and the correlation coefficient was 0.707; triglyceride was positively correlated with TH. The coefficient was 0.590 (P 0.05), indicating that T cell subsets were closely related to renal injury and dyslipidemia. Conclusion: 1. Preeclampsia and T cell subsets were correlated. TH, TH / TS in severe preeclampsia patients increased significantly, TS decreased significantly. 2. Uric acid, total cholesterol, triglyceride levels in severe preeclampsia patients increased significantly, glomerular filtration rate was clear. The correlation analysis showed that uric acid, glomerular filtration rate, total cholesterol and triglyceride levels were correlated with T cell subsets in severe pre-eclampsia patients.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244
[Abstract]:Objective: Preeclampsia PE is a kind of pregnancy-specific complications characterized by systemic vasospasm, inflammation and kidney injury. To study the correlation between T cell subsets and preeclampsia and the effect of different proportion of T cell subsets on renal function and blood lipids in preeclampsia patients. From July 2016 to January 2017, the clinical data of 24 pregnant women with severe preeclampsia (Group A), 21 mild preeclampsia (Group B) and 24 normal pregnant women (Group C) were analyzed. The T lymphocyte subsets of pregnant women were detected by flow cytometry. SPSS19.0 was used to test the single factor variance and Pearson correlation coefficient. Results: 1. Compared with severe preeclampsia group (group A), mild preeclampsia group (group B) and normal pregnancy group (group C), there was no significant difference in the mean level of age and pregnancy between groups. There was no significant difference between severe preeclampsia group and mild preeclampsia group (P 0.05). The mean gestational age of severe preeclampsia group and mild preeclampsia group had significant statistical significance (P 0.05). However, there was no significant difference between severe preeclampsia group and mild preeclampsia group (P 0.05). Compared with mild preeclampsia group and normal pregnancy group, the number of CD4 + T cells (TH) in severe preeclampsia group was significantly higher (P 0.05), while the number of CD8 + T cells (TS) was significantly lower than the mean level of normal pregnancy group (P 0.05), so the ratio of CD4 + cell number to CD8 + cell number (TH / TS), severe preeclampsia group and mild preeclampsia group and normal pregnancy was significantly lower. The levels of TH / TS and uric acid in severe preeclampsia group were significantly higher than those in mild preeclampsia group (P 0.05). The levels of uric acid in severe preeclampsia group were significantly higher than those in mild preeclampsia group and normal preeclampsia group (P 0.05). Compared with normal pregnancy group, the mean level of uric acid was not significantly different (P 0.05); glomerular filtration rate (GFR): Compared with mild preeclampsia group and normal pregnancy group, the mean level of GFR in severe preeclampsia group was significantly lower (P 0.05), but there was no significant difference between mild preeclampsia group and normal pregnancy group (P 0.0). 5) Total cholesterol level: compared with severe preeclampsia group, normal pregnancy group and mild preeclampsia group, the former two groups were slightly higher than the latter (P 0.05), and the former two groups were also higher than the normal range; there was no significant difference between severe preeclampsia group and normal pregnancy group (P 0.05); triglyceride level: the mean values of the three groups were significantly higher than the normal range, preeclampsia group were significantly higher than the normal range. In severe preeclampsia group, the levels of uric acid, triglyceride and total cholesterol in severe preeclampsia group were not significantly different from those in mild preeclampsia group (P 0.05). The serum uric acid level was positively correlated with TH, and the correlation coefficient was 0.752 (P 0.05); GFR was negatively correlated with TH / TS, and the correlation coefficient was - 0.601 (P 0.05); total cholesterol was positively correlated with TH (P 0.05), and the correlation coefficient was 0.707; triglyceride was positively correlated with TH. The coefficient was 0.590 (P 0.05), indicating that T cell subsets were closely related to renal injury and dyslipidemia. Conclusion: 1. Preeclampsia and T cell subsets were correlated. TH, TH / TS in severe preeclampsia patients increased significantly, TS decreased significantly. 2. Uric acid, total cholesterol, triglyceride levels in severe preeclampsia patients increased significantly, glomerular filtration rate was clear. The correlation analysis showed that uric acid, glomerular filtration rate, total cholesterol and triglyceride levels were correlated with T cell subsets in severe pre-eclampsia patients.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244
【相似文献】
相关期刊论文 前10条
1 桑翠琴;张震宇;王淑珍;刘浩;郭淑丽;;重度子痫前期/子痫合并急性呼吸窘迫综合征的诊断与处理[J];中国医刊;2006年10期
2 赵桂花;;重度子痫前期与子痫82例临床分析[J];临床医药实践杂志;2007年01期
3 杨小颀;严妮子;;子痫前期与血钙水平相关性研究[J];实用临床医学;2007年04期
4 杨小颀;严妮子;;子痫前期与血钙水平相关性研究[J];四川生理科学杂志;2007年01期
5 陈茜;王泽华;;子痫前期的预测及预防[J];华中医学杂志;2007年03期
6 朱倩;;对38例子痫前期(重)、子痫治愈的体会[J];实用预防医学;2007年05期
7 梁英;周丽丽;甘娟;姜碧洋;杨承东;;重度子痫前期在产科ICU监护救治的临床分析[J];中国妇幼保健;2008年19期
8 詹卫星;郑九生;;血管紧张素转换酶基因多态性与重度子痫前期及肾功能损害的关系[J];江西医药;2008年08期
9 蔡颖;尹国武;姜锋;李怡;闵保华;王s，
本文编号：2205104
本文链接：https://www.wllwen.com/yixuelunwen/fuchankeerkelunwen/2205104.html
最近更新
教材专著
