DAPT抑制妊娠滋养细胞肿瘤Notch信号通路对细胞增殖和EMT影响的研究
发布时间：2018-08-28 17:29
【摘要】：目的:探讨Notch信号通路在人妊娠滋养细胞肿瘤发生发展过程中的重要作用,及其与EMT之间的关系。方法:采用γ-分泌酶抑制剂DAPT处理JAR和JEG-3两种人绒毛膜癌细胞株,MTT法检测两种细胞株的增殖情况,q PCR法检测Notch1 mRNA表达。Western blot法检测DAPT阻断Notch信号通路后JAR细胞中EMT相关蛋白E-cad的表达情况。结果:DAPT能抑制JEG-3、JAR细胞生长,10μmol/L和15μmol/L DAPT作用48h开始分别抑制JEG-3和JAR细胞生长(P0.05),且抑制作用呈时间和浓度依赖关系。JEG-3细胞中,1μmol/L和10μmol/L DAPT作用组中Notch1 mRNA表达下调,两组比较差异无统计学意义(P0.05);JAR细胞中,10μmol/L和15μmol/L DAPT作用组中Notch1 mRNA表达下调,两组比较差异有统计学意义(P0.05)。DAPT能上调JAR细胞中E-cad表达。结论:Notch信号通路和EMT与妊娠滋养细胞肿瘤的发生发展有关;γ-分泌酶抑制剂DAPT或可成为妊娠滋养细胞肿瘤治疗的一个新靶点。
[Abstract]:Aim: to investigate the important role of Notch signaling pathway in the development of human gestational trophoblastic tumor and its relationship with EMT. Methods: the proliferation of two kinds of human choriocarcinoma cell lines (JAR and JEG-3) was detected by DAPT, a 纬 -secretory enzyme inhibitor. The expression of Notch1 mRNA was detected by q-PCR and EMT in JAR cells after DAPT blocked Notch signaling pathway was detected by Western blot. Expression of related protein E-cad. Results the growth of JEG-3,JAR cells was inhibited by 10 渭 mol/L and 15 渭 mol/L DAPT for 48 h, respectively (P0.05). The inhibitory effect was in a time-and concentration-dependent manner. Notch1 mRNA expression was down-regulated in JEG-3 cells treated with 1 渭 mol/L and 10 渭 mol/L DAPT, respectively. There was no significant difference between the two groups (P0.05) the expression of Notch1 mRNA was down-regulated in the group treated with 10 渭 mol/L and 15 渭 mol/L DAPT in jar cells, and the difference between the two groups was statistically significant (P0.05) .DAPT could up-regulate the expression of E-cad in JAR cells. Conclusion the EMT and the signal pathway of Notch are related to the development of gestational trophoblastic tumors, and 纬 -secretase inhibitor DAPT may be a new target for the treatment of gestational trophoblastic tumors.
【作者单位】： 西安交通大学医学院第一附属医院妇产科;
【基金】：国家自然科学基金资助项目(No:81172489)
【分类号】：R737.33
[Abstract]:Aim: to investigate the important role of Notch signaling pathway in the development of human gestational trophoblastic tumor and its relationship with EMT. Methods: the proliferation of two kinds of human choriocarcinoma cell lines (JAR and JEG-3) was detected by DAPT, a 纬 -secretory enzyme inhibitor. The expression of Notch1 mRNA was detected by q-PCR and EMT in JAR cells after DAPT blocked Notch signaling pathway was detected by Western blot. Expression of related protein E-cad. Results the growth of JEG-3,JAR cells was inhibited by 10 渭 mol/L and 15 渭 mol/L DAPT for 48 h, respectively (P0.05). The inhibitory effect was in a time-and concentration-dependent manner. Notch1 mRNA expression was down-regulated in JEG-3 cells treated with 1 渭 mol/L and 10 渭 mol/L DAPT, respectively. There was no significant difference between the two groups (P0.05) the expression of Notch1 mRNA was down-regulated in the group treated with 10 渭 mol/L and 15 渭 mol/L DAPT in jar cells, and the difference between the two groups was statistically significant (P0.05) .DAPT could up-regulate the expression of E-cad in JAR cells. Conclusion the EMT and the signal pathway of Notch are related to the development of gestational trophoblastic tumors, and 纬 -secretase inhibitor DAPT may be a new target for the treatment of gestational trophoblastic tumors.
【作者单位】： 西安交通大学医学院第一附属医院妇产科;
【基金】：国家自然科学基金资助项目(No:81172489)
【分类号】：R737.33
【参考文献】
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1 邹晓英;庄Y，
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