宫颈上皮瘤变中P16蛋白表达及基因外显子缺失的意义
发布时间：2018-08-30 20:07
【摘要】：研究背景： P16是肿瘤抑制基因,其编码产生的P16蛋白直接参与细胞周期的调节，p16蛋白与多种肿瘤相关。P16通过缺失、高甲基化和点突变导致基因失活在肿瘤的发生发展中发挥重要作用。 目的： 1.探讨P16蛋白在宫颈上皮内瘤变的表达及相关性。 2.研究P16基因外显子的缺失与宫颈上皮内瘤变的关系。 方法： 1.免疫组化染色检测P16蛋白的表达情况：选取福建医科大学附属第一医院2011年6月-2013年2月宫颈手术切除及活检标本407例，包括正常宫颈鳞状上皮81例、CIN I95例、CIN II74例、CINIII77例、宫颈癌80例。 2. PCR扩增法检测P16基因外显1、2和3缺失情况：选取福建医科大学附属第一医院2011年6月-2013年2月宫颈手术切除标本80例，包括正常宫颈鳞状上皮10例、CIN I10例、CIN II20例、CINIII20例、宫颈癌20例。 结果： 1．随着宫颈鳞状上皮瘤变的进展，P16蛋白表达率逐渐升高：CIN Ⅰ组中阳性表达率为31.58%，，CIN Ⅱ组中阳性表达率为62.16%，CIN Ⅲ组中阳性表达率为93.50%，癌组中阳性表达率为100.00% 2.随着宫颈鳞状上皮瘤变的进展，P16蛋白表达范围逐渐扩大：：CIN Ⅰ级阳性细胞主要局限于鳞状上皮下1/3层；CIN Ⅱ级扩展至鳞状上皮的下2/3；CIN Ⅲ级阳性细胞表达于上皮全层。 3.随着宫颈鳞状上皮瘤变的进展，P16蛋白强阳性表达率逐渐增加：CINⅠ级为1.05%，CINⅡ级为5.41%，CINⅢ为50.65%，癌中p16强阳性表达率为85.00%。 4.在宫颈鳞状上皮瘤变过程中，P16基因发生外显子缺失：共检测39例外显子缺失的病例，其中11例外显子1缺失、26例外显子2缺失及2例外显子3缺失。9例同时存在外显子1和2缺失。 5. P16基因外显子2的缺失率显著高于外显子1和外显子3的缺失率。结论 1. P16蛋白表达参与宫颈鳞状上皮瘤变的进展过程，免疫组化染色结果可以协助判断宫颈病变情况。 2. P16基因外显子的缺失可能参与与宫颈鳞状上皮的瘤变。
[Abstract]:Background: P16 is a tumor suppressor gene. The P16 protein encoded by P16 protein is directly involved in the regulation of cell cycle. P16 protein is associated with a variety of tumors through deletion. Gene inactivation due to hypermethylation and point mutation plays an important role in tumorigenesis and development. Objective: 1. To investigate the expression and correlation of P16 protein in cervical intraepithelial neoplasia. 2. To study the relationship between deletion of P16 gene exon and cervical intraepithelial neoplasia. Methods: 1. Immunohistochemical staining was used to detect the expression of P16 protein. From June 2011 to February 2013, 407 specimens of cervical excision and biopsy were collected from the first affiliated Hospital of Fujian Medical University, including 81 cases of normal cervical squamous epithelium, 95 cases of cin I and 77 cases of cin II I. 80 cases of cervical cancer PCR amplification method was used to detect the deletion of P16 gene. 80 cases of cervical excision were selected from the first affiliated Hospital of Fujian Medical University from June 2011 to February 2013, including 10 cases of cervical squamous epithelium with cin I 10 cases of cin II20 and 20 cases of CINIII I. There were 20 cases of cervical cancer. Results: 1. With the progression of cervical squamous epithelioma, the expression rate of P16 protein increased gradually. The positive expression rate of P16 protein was 31.58% in cin 鈪
本文编号：2214150
[Abstract]:Background: P16 is a tumor suppressor gene. The P16 protein encoded by P16 protein is directly involved in the regulation of cell cycle. P16 protein is associated with a variety of tumors through deletion. Gene inactivation due to hypermethylation and point mutation plays an important role in tumorigenesis and development. Objective: 1. To investigate the expression and correlation of P16 protein in cervical intraepithelial neoplasia. 2. To study the relationship between deletion of P16 gene exon and cervical intraepithelial neoplasia. Methods: 1. Immunohistochemical staining was used to detect the expression of P16 protein. From June 2011 to February 2013, 407 specimens of cervical excision and biopsy were collected from the first affiliated Hospital of Fujian Medical University, including 81 cases of normal cervical squamous epithelium, 95 cases of cin I and 77 cases of cin II I. 80 cases of cervical cancer PCR amplification method was used to detect the deletion of P16 gene. 80 cases of cervical excision were selected from the first affiliated Hospital of Fujian Medical University from June 2011 to February 2013, including 10 cases of cervical squamous epithelium with cin I 10 cases of cin II20 and 20 cases of CINIII I. There were 20 cases of cervical cancer. Results: 1. With the progression of cervical squamous epithelioma, the expression rate of P16 protein increased gradually. The positive expression rate of P16 protein was 31.58% in cin 鈪
本文编号：2214150
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