B7-H4和HK6在上皮性卵巢肿瘤中的表达及临床意义
发布时间：2018-09-02 08:06
【摘要】：目的 通过检测B7-H4蛋白和HK6蛋白在正常卵巢组织、卵巢上皮性良性肿瘤组织、交界性肿瘤组织以及恶性肿瘤组织中的表达水平，探讨B7-H4蛋白和HK6蛋白在上皮性卵巢肿瘤的发生发展中的可能作用及其临床意义。研究方法 本研究以70例卵巢上皮性肿瘤组织为研究对象，以10例正常卵巢组织为对照组，采用免疫组织化学染色SP法检测B7-H4蛋白和HK6蛋白的表达，比较它们在正常卵巢组织、卵巢上皮性良性、交界性及恶性肿瘤组织中的阳性表达情况；并对二者在卵巢上皮性恶性肿瘤组织中的表达情况与患者的临床病理参数之间的关系进行统计学分析。 结果 1、B7-H4蛋白在正常卵巢组织、卵巢上皮性良性、交界性及恶性肿瘤组织中的阳性表达率分别为0.0%（0/10）、20.0%（3/15）、30.0%（3/10）、80.0%（36/45），在卵巢上皮性恶性肿瘤组的阳性表达率明显高于前三组，差异有统计学意义（χ2=31.192，P=0.000），而卵巢上皮性良性肿瘤组与交界性肿瘤组相比较，差异无统计学意义（χ2=0.009，P=0.924）。 2、B7-H4蛋白在卵巢上皮性恶性肿瘤组织中的表达： （1）≤50岁组的阳性表达率为75.0%（6/8），＞50岁组的阳性表达率为81.1%（30/37），两者比较差异无统计学意义（χ2=0.000，P=1.000）； （2）浆液性癌、黏液性癌、子宫内膜样癌和透明细胞癌中的阳性表达率分别为96.2%（25/26）、28.6%（2/7）、83.8%（5/6）和66.7%（4/6），在黏液性癌组的阳性表达率为28.6%（2/7），明显低于非粘液性癌组89.5%（34/38），差异有统计学意义（χ2=10.161，P=0.001），而非粘液性癌各组之间比较差异无统计学意义（χ2=4.052，P=0.132）； （3）Ⅰ~Ⅱ期的阳性表达率为52.9%（9/17），明显低于Ⅲ~Ⅳ期96.5%（27/28），差异具有统计学意义（χ2=9.932，P=0.002）； （4）高分化（G1）组阳性表达率为37.5%（3/8），明显低于中（G2）、低分化（G3）组89.2%（33/37），差异具有统计学意义（χ2=10.984，P=0.001）； （5）有淋巴结转移组的阳性表达率为94.7%（18/19），高于无淋巴结转移组69.2%（18/26），差异具有统计学意义（χ2=4.464，P=0.035）； （6）有腹水组的阳性表达率为85.7%（12/14），无腹水组的阳性表达率为77.4%（24/31），两者比较差异无统计学意义（χ2=0.058，P=0.809）。 3、HK6蛋白在正常卵巢组织、卵巢上皮性良性、交界性及恶性肿瘤组织中的阳性表达率分别为10.0%（1/10）、13.3%（2/15）、50.0%（5/10）和60.0%（27/45）。HK6蛋白在卵巢上皮性良性肿瘤组中的阳性表达率明显低于交界性及恶性肿瘤组，差异有统计学意义（χ2=9.490，P=0.002），而交界性肿瘤组与恶性肿瘤组相比较，差异无统计学意义（χ2=0.051，P=0.822）。 4、HK6蛋白在卵巢恶性肿瘤组织中的表达： （1）≤50岁组的阳性表达率为62.5%（5/8），＞50岁组的阳性表达率为59.5%（22/37），两者比较差异无统计学意义（χ2=0.000，P=1.000）； （2）浆液性癌、黏液性癌、子宫内膜样癌和透明细胞癌中的阳性表达率分别为65.4%（17/26）、57.1%（4/7）、50.0%（3/6）和50.0%（3/6），各种不同组织学类型间相互比较，差异无统计学意义（χ2=0.833，P=0.842）； （3）Ⅰ~Ⅱ期的阳性表达率为35.3%（6/17），低于Ⅲ~Ⅳ期75.0%（21/28），差异有统计学意义（χ2=6.949，P=0.008）； （4）高分化（G1）组的阳性表达率为12.5%（l/8），明显低于中（G2）、低分化（G3）组70.3%（26/37），差异具有统计学意义（χ2=6.898，P=0.009）； （5）有淋巴结转移组的阳性表达率为84.2%（16/19），，高于无淋巴结转移组42.3%（11/26），差异具有统计学意义（χ2=8.031，P=0.005）。 （6）有腹水组的阳性表达率为64.3%（9/14），无腹水组的阳性表达率为58.2%(18/31)，两者比较差异无统计学意义（χ2=0.004，P=0.948）。 5、B7-H4蛋白和HK6蛋白的表达呈低度线性正相关关系（r=0.218，P=0.039）。 结论 1、B7-H4蛋白和HK6蛋白在卵巢上皮性肿瘤组织中均较高水平表达，且表达强度随病理分期增加而增强，提示B7-H4蛋白和HK6蛋白可能参与卵巢上皮性肿瘤的进展。 2、在卵巢上皮性恶性肿瘤组织中B7-H4蛋白和HK6蛋白的表达程度均与病理分期、病理分级及淋巴结转移有关，而与患者年龄及腹水无关。B7-H4蛋白的表达还与组织学类型有关，HK6蛋白的表达则与之无关。 3、B7-H4蛋白和HK6蛋白的表达间存在一定的线性正相关关系，说明B7-H4蛋白和HK6蛋白可能协同参与了卵巢上皮性恶性肿瘤的发生和发展。
[Abstract]:objective
The expression of B7-H4 protein and HK6 protein in normal ovarian tissues, ovarian epithelial benign tumor tissues, borderline tumor tissues and malignant tumor tissues were detected to explore the possible role of B7-H4 protein and HK6 protein in the occurrence and development of epithelial ovarian tumors and its clinical significance.
The expression of B7-H4 protein and HK6 protein in 70 cases of ovarian epithelial tumors and 10 cases of normal ovarian tissues were detected by immunohistochemical staining SP method. The positive expression of B7-H4 protein and HK6 protein in normal ovarian tissues, ovarian epithelial benign, borderline and malignant ovarian tissues were compared. Statistical analysis was made on the relationship between the expression of the protein in ovarian epithelial malignant tumors and the clinicopathological parameters.
Result
1. The positive rates of B7-H4 protein in normal ovarian tissues, benign epithelial ovarian tissues, borderline ovarian tissues and malignant tumors were 0.0% (0/10), 20.0% (3/15), 30.0% (3/10) and 80.0% (36/45), respectively. The positive rates in ovarian epithelial malignant tumors were significantly higher than those in the first three groups (2 = 31.192, P = 0.000). There was no significant difference between benign tumor group and borderline tumor group (x 2=0.009, P=0.924).
2, the expression of B7-H4 protein in epithelial ovarian cancer tissues:
(1) The positive expression rate was 75.0% (6/8) in the group under 50 years old and 81.1% (30/37) in the group over 50 years old. There was no significant difference between the two groups (_2 = 0.000, P = 1.000).
(2) The positive expression rates in serous carcinoma, mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma were 96.2% (25/26), 28.6% (2/7), 83.8% (5/6) and 66.7% (4/6), respectively. The positive expression rates in mucinous carcinoma group were 28.6% (2/7), significantly lower than those in non-mucinous carcinoma group (89.5% (34/38), and the difference was statistically significant (2 = 10.161, P = 0.001). There was no significant difference among groups (chi 2=4.052, P=0.132).
(3) The positive expression rate of stage I-II was 52.9% (9/17), significantly lower than that of stage III-IV (96.5% (27/28), and the difference was statistically significant (_2 = 9.932, P = 0.002).
(4) The positive expression rate in G1 group was 37.5% (3/8), significantly lower than that in G2 group and 89.2% (33/37) in G3 group. The difference was statistically significant (2 = 10.984, P = 0.001).
(5) The positive rate of lymph node metastasis group was 94.7% (18/19), which was higher than that of non-lymph node metastasis group (69.2% (18/26), and the difference was statistically significant (_2 = 4.464, P = 0.035).
(6) The positive expression rate was 85.7% (12/14) in ascites group and 77.4% (24/31) in non-ascites group. There was no significant difference between the two groups (_2 = 0.058, P = 0.809).
3. The positive rates of HK6 protein in normal ovarian tissues, benign epithelial ovarian tissues, borderline ovarian tissues and malignant tumors were 10.0% (1/10), 13.3% (2/15), 50.0% (5/10) and 60.0% (27/45), respectively. The positive rates of HK6 protein in benign ovarian epithelial tumors were significantly lower than those in borderline ovarian tissues and malignant ovarian tumors (2=9). There was no significant difference between the borderline tumor group and the malignant tumor group (_2 = 0.051, P = 0.822).
4, the expression of HK6 protein in ovarian cancer tissues:
(1) The positive expression rate was 62.5% (5/8) in the group under 50 years old and 59.5% (22/37) in the group over 50 years old. There was no significant difference between the two groups (_2 = 0.000, P = 1.000).
(2) The positive expression rates of serous carcinoma, mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma were 65.4% (17/26), 57.1% (4/7), 50.0% (3/6) and 50.0% (3/6), respectively. There was no significant difference between different histological types (2 = 0.833, P = 0.842).
(3) The positive rate of stage I-II was 35.3% (6/17), lower than 75.0% (21/28) in stage III-IV, and the difference was statistically significant (_2 = 6.949, P = 0.008).
(4) The positive expression rate of G1 group was 12.5% (l/8), significantly lower than that of G2 group and 70.3% (26/37) in G3 group. The difference was statistically significant (2 = 6.898, P = 0.009).
(5) The positive rate of lymph node metastasis group was 84.2% (16/19), which was higher than that of non-lymph node metastasis group (42.3% (11/26), and the difference was statistically significant (_2 = 8.031, P = 0.005).
(6) The positive expression rate was 64.3% (9/14) in ascites group and 58.2% (18/31) in non-ascites group. There was no significant difference between the two groups (_2 = 0.004, P = 0.948).
5, there was a low positive linear correlation between B7-H4 protein and HK6 protein (r=0.218, P=0.039).
conclusion
1, B7-H4 protein and HK6 protein were highly expressed in ovarian epithelial tumors, and the expression intensity increased with the pathological stage, suggesting that B7-H4 protein and HK6 protein may be involved in the progression of ovarian epithelial tumors.
2. The expression of B7-H4 and HK6 proteins in ovarian epithelial malignant tumors was correlated with pathological stage, pathological grade and lymph node metastasis, but not with age and ascites. The expression of B7-H4 protein was correlated with histological type, but not with HK6 protein.
3. There is a linear positive correlation between the expression of B7-H4 protein and HK6 protein, indicating that B7-H4 protein and HK6 protein may be involved in the occurrence and development of ovarian epithelial malignancies.
【学位授予单位】：泰山医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.31
本文编号：2218762
[Abstract]:objective
The expression of B7-H4 protein and HK6 protein in normal ovarian tissues, ovarian epithelial benign tumor tissues, borderline tumor tissues and malignant tumor tissues were detected to explore the possible role of B7-H4 protein and HK6 protein in the occurrence and development of epithelial ovarian tumors and its clinical significance.
The expression of B7-H4 protein and HK6 protein in 70 cases of ovarian epithelial tumors and 10 cases of normal ovarian tissues were detected by immunohistochemical staining SP method. The positive expression of B7-H4 protein and HK6 protein in normal ovarian tissues, ovarian epithelial benign, borderline and malignant ovarian tissues were compared. Statistical analysis was made on the relationship between the expression of the protein in ovarian epithelial malignant tumors and the clinicopathological parameters.
Result
1. The positive rates of B7-H4 protein in normal ovarian tissues, benign epithelial ovarian tissues, borderline ovarian tissues and malignant tumors were 0.0% (0/10), 20.0% (3/15), 30.0% (3/10) and 80.0% (36/45), respectively. The positive rates in ovarian epithelial malignant tumors were significantly higher than those in the first three groups (2 = 31.192, P = 0.000). There was no significant difference between benign tumor group and borderline tumor group (x 2=0.009, P=0.924).
2, the expression of B7-H4 protein in epithelial ovarian cancer tissues:
(1) The positive expression rate was 75.0% (6/8) in the group under 50 years old and 81.1% (30/37) in the group over 50 years old. There was no significant difference between the two groups (_2 = 0.000, P = 1.000).
(2) The positive expression rates in serous carcinoma, mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma were 96.2% (25/26), 28.6% (2/7), 83.8% (5/6) and 66.7% (4/6), respectively. The positive expression rates in mucinous carcinoma group were 28.6% (2/7), significantly lower than those in non-mucinous carcinoma group (89.5% (34/38), and the difference was statistically significant (2 = 10.161, P = 0.001). There was no significant difference among groups (chi 2=4.052, P=0.132).
(3) The positive expression rate of stage I-II was 52.9% (9/17), significantly lower than that of stage III-IV (96.5% (27/28), and the difference was statistically significant (_2 = 9.932, P = 0.002).
(4) The positive expression rate in G1 group was 37.5% (3/8), significantly lower than that in G2 group and 89.2% (33/37) in G3 group. The difference was statistically significant (2 = 10.984, P = 0.001).
(5) The positive rate of lymph node metastasis group was 94.7% (18/19), which was higher than that of non-lymph node metastasis group (69.2% (18/26), and the difference was statistically significant (_2 = 4.464, P = 0.035).
(6) The positive expression rate was 85.7% (12/14) in ascites group and 77.4% (24/31) in non-ascites group. There was no significant difference between the two groups (_2 = 0.058, P = 0.809).
3. The positive rates of HK6 protein in normal ovarian tissues, benign epithelial ovarian tissues, borderline ovarian tissues and malignant tumors were 10.0% (1/10), 13.3% (2/15), 50.0% (5/10) and 60.0% (27/45), respectively. The positive rates of HK6 protein in benign ovarian epithelial tumors were significantly lower than those in borderline ovarian tissues and malignant ovarian tumors (2=9). There was no significant difference between the borderline tumor group and the malignant tumor group (_2 = 0.051, P = 0.822).
4, the expression of HK6 protein in ovarian cancer tissues:
(1) The positive expression rate was 62.5% (5/8) in the group under 50 years old and 59.5% (22/37) in the group over 50 years old. There was no significant difference between the two groups (_2 = 0.000, P = 1.000).
(2) The positive expression rates of serous carcinoma, mucinous carcinoma, endometrioid carcinoma and clear cell carcinoma were 65.4% (17/26), 57.1% (4/7), 50.0% (3/6) and 50.0% (3/6), respectively. There was no significant difference between different histological types (2 = 0.833, P = 0.842).
(3) The positive rate of stage I-II was 35.3% (6/17), lower than 75.0% (21/28) in stage III-IV, and the difference was statistically significant (_2 = 6.949, P = 0.008).
(4) The positive expression rate of G1 group was 12.5% (l/8), significantly lower than that of G2 group and 70.3% (26/37) in G3 group. The difference was statistically significant (2 = 6.898, P = 0.009).
(5) The positive rate of lymph node metastasis group was 84.2% (16/19), which was higher than that of non-lymph node metastasis group (42.3% (11/26), and the difference was statistically significant (_2 = 8.031, P = 0.005).
(6) The positive expression rate was 64.3% (9/14) in ascites group and 58.2% (18/31) in non-ascites group. There was no significant difference between the two groups (_2 = 0.004, P = 0.948).
5, there was a low positive linear correlation between B7-H4 protein and HK6 protein (r=0.218, P=0.039).
conclusion
1, B7-H4 protein and HK6 protein were highly expressed in ovarian epithelial tumors, and the expression intensity increased with the pathological stage, suggesting that B7-H4 protein and HK6 protein may be involved in the progression of ovarian epithelial tumors.
2. The expression of B7-H4 and HK6 proteins in ovarian epithelial malignant tumors was correlated with pathological stage, pathological grade and lymph node metastasis, but not with age and ascites. The expression of B7-H4 protein was correlated with histological type, but not with HK6 protein.
3. There is a linear positive correlation between the expression of B7-H4 protein and HK6 protein, indicating that B7-H4 protein and HK6 protein may be involved in the occurrence and development of ovarian epithelial malignancies.
【学位授予单位】：泰山医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.31
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