坤泰胶囊对促排卵小鼠子宫内膜溶血磷脂酸受体3、环氧合酶2表达的影响

发布时间：2018-09-07 14:38

【摘要】：目的观察坤泰胶囊对促排卵小鼠子宫内膜溶血磷脂酸受体3(LPAR3)、环氧合酶2(COX-2)表达的影响,探讨其是否具有提高子宫内膜容受性的作用。方法将90只6~8周龄的昆明雌鼠随机分为三组,各30只。A组:每天生理盐水灌胃1次(连续1个月),控制性超促排卵(COH);B组:每天坤泰胶囊生理盐水溶解后灌胃1次(连续1个月),COH;C组:每天生理盐水灌胃1次(连续1个月)。雌鼠与雄鼠以2∶1合笼,检查雌鼠次日晨阴栓(按天数表示为d1~d6)。每天每组处死5只雌鼠,取其子宫,观察子宫内膜腺体发育情况,检测其LPAR3、COX-2。结果 d4,A、B、C组子宫内膜腺体数分别为(13±3)、(20±2)、C(17±2)个,B组与A、C组相比,P均0.05。d3,A、B、C组子宫内膜LPAR3的积分光密度值(IOD值)分别为0.293±0.019、0.255±0.014、0.253±0.032,B组与A组相比,P0.05;d4,A、B、C组子宫内膜LPAR3的IOD值分别为0.207±0.022、0.330±0.026、0.311±0.019,B组与A组相比,P0.05。d4、d5、d6,A组子宫内膜COX-2的IOD值分别为0.282±0.019、0.419±0.052、0.573±0.057,B组分别为0.176±0.018、0.288±0.020、0.417±0.053,两组相比,P均0.05。结论坤泰胶囊可调控COH小鼠子宫内膜LPAR3及COX-2的表达,发挥提高子宫内膜容受性的作用。
[Abstract]:Objective to observe the effect of Kun Tai capsule on the expression of lysophosphatidic acid receptor 3 (LPAR3) and cyclooxygenase-2 (COX-2) in endometrium of ovulation promoting mice. Methods 90 female Kunming rats aged 6 and 8 weeks were randomly divided into three groups. 30 rats in group A were treated with normal saline once a day (1 month in succession), and those in group B of controlled hyperstimulation of ovulation (COH) group B) were treated with Kundai capsule once a day (1 month in a row) after dissolution of normal saline (continuous 1 month): group C (group C): once a day (1 month in succession). Female and male mice were caged at 2:1 to check the morning Yin suppository of female rats (expressed as d1~d6 by the number of days). Every day, 5 female rats were killed in each group, their uterus was taken, the development of endometrial glands was observed and their LPAR3,COX-2. was detected. Results the number of endometrial glands in group C was (13 卤3), (, 20 卤2), and (17 卤2) in group B, compared with that in group A, the integrated optical density (IOD) of endometrial LPAR3 in group B was 0.293 卤0.0190.255 卤0.0140.253 卤0.032, respectively, which was 0.207 卤0.022 卤0.330 卤0.026 卤0.311 卤0.019 卤0.311 卤0.019 卤0.311 卤0.019 in group A and group A, respectively. The IOD values of endometrial LPAR3 in group C were 0.207 卤0.022 卤0.330 卤0.026 卤0.311 卤0.311 卤0.019 respectively. The IOD values of endometrial COX-2 in group A were 0.282 卤0.019 卤0.052 卤0.573 卤0.057 and 0.176 卤0.018, 0.288 卤0.020, 0.417 卤0.053, respectively. Conclusion Kun Tai capsule can regulate the expression of LPAR3 and COX-2 in endometrium of COH mice and improve the endometrial receptivity.
【作者单位】：新乡医学院第三附属医院;
【基金】：河南省高等学校骨干教师科研基金资助项目(2012GGJS-135)
【分类号】：R714.8

【相似文献】