配对盒家族基因1甲基化检测在子宫颈癌诊断中的应用

发布时间：2018-09-07 13:17

【摘要】：目的检测配对盒家族基因1(PAX1)在不同宫颈病变宫颈脱落细胞中的甲基化水平,评估其作为宫颈上皮细胞癌变分子标志物的价值。方法采用焦磷酸测序法检测PAX1基因的甲基化情况。比较正常组织与低级别宫颈鳞状上皮内病变(LSIL)、高级别宫颈鳞状上皮内病变(HSIL)和宫颈癌组织(各组20例)甲基化水平的差异。并采用受试者工作特性曲线(ROC)确定癌变组与非癌变组之间的判别临界值。结果 1正常宫颈组PAX1基因甲基化水平为(4.57±2.43)%,LSIL组为(3.383±2.364)%,HSIL组为(13.64±13.35)%,宫颈癌组为(26.39±18.53)%,其中正常宫颈组与LSIL组PAX1基因甲基化水平比较,差异无统计学意义(P0.05),其他各组间比较,差异均有统计学意义(P0.05)。2 ROC曲线下面积(AUC)为0.893,提示有较高的诊断价值。取PAX1甲基化水平27%作为临界值,检测灵敏度为97.1%,特异度为85.2%。结论焦磷酸测序法PAX1基因甲基化水平定量检测对于宫颈癌早期临床诊断具有潜在的诊断价值。
[Abstract]:Objective to detect the methylation level of pairing box family gene 1 (PAX1) in cervical exfoliated cells from different cervical lesions and to evaluate its value as a molecular marker of cervical epithelial cell carcinogenesis. Methods the methylation of PAX1 gene was detected by pyrosequencing. The methylation levels of high grade cervical squamous intraepithelial lesions (HSIL) and cervical carcinoma (20 cases) were compared between normal and low grade cervical squamous intraepithelial lesions (LSIL),). The critical value between canceration group and non-cancerous group was determined by using the operating characteristic curve (ROC). Results 1 the methylation level of PAX1 gene was (4.57 卤2.43) and (3.383 卤2.364) in normal cervix group and (13.64 卤13.35) in cervical cancer group, and (26.39 卤18.53) in cervical cancer group. There was no significant difference in PAX1 methylation between normal cervix group and LSIL group (P0.05). The difference was statistically significant (P0.05). The area (AUC) under the ROC curve was 0.893, indicating that it had higher diagnostic value. The PAX1 methylation level was taken as the critical value, the sensitivity was 97.1and the specificity was 85.2. Conclusion the quantitative detection of methylation level of PAX1 gene by pyrosequencing has potential diagnostic value for early clinical diagnosis of cervical cancer.
【作者单位】：江苏省苏州市立医院妇产科;
【基金】：南京医科大学科技发展基金面上项目(09NJMUM133)
【分类号】：R737.33

【相似文献】