不同剂量戊酸雌二醇防治大鼠宫腔粘连的作用研究

发布时间：2018-09-09 15:43

【摘要】：目的:研究不同剂量的戊酸雌二醇(estradiol valerate,E2V)对大鼠宫腔粘连(intrauterine adhesions,IUA)的防治效果。方法:将48只8~10周龄健康雌性SD大鼠随机分为8组:空白对照组、实验对照组、3个雌激素预防组(低、中、高剂量)、3个雌激素治疗组(低、中、高剂量)。收集各组大鼠子宫组织,HE染色观察子宫内膜腺体,MASSON染色观察子宫内膜纤维化程度,免疫组化法检测子宫内膜转化生长因子-β1(transforming growth factor-β1,TGF-β1)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)、雌激素受体-α(estrogen receptor-α,ER-α)的表达水平。结果:与实验对照组相比,3个雌激素预防组大鼠的子宫内膜腺体计数增多(P值均为0.000)、纤维化面积降低(P_(低雌A组)=0.013,P_(中雌A组)=0.021,P_(高雌A组)=0.003),差异均有统计学意义,3个雌激素治疗组大鼠的子宫内膜腺体计数(P_(低雌B组)=0.536,P_(中雌B组)=0.536,P_(高雌B组)=1.000)及纤维化面积(P值均为1.000)与实验对照组相比均无显著性差异;6个雌激素组的子宫内膜TGF-β1表达水平均降低(P值均为0.000),其中中、高剂量组均低于低剂量组(P值分别为0.003及0.002),而中、高剂量2组相比无差异(P=0.842);6个雌激素组子宫内膜MMP-9表达水平均升高(P值均为0.000),中、高剂量组均高于低剂量组(P值均为0.000),而中、高剂量2组相比无差异(P=0.379);6个雌激素组子宫内膜ER-α表达水平均有升高(P值均为0.000),中、高剂量组均高于低剂量组(P值均为0.000),而中、高剂量2组相比无差异(P=0.513)。结论:在大鼠IUA建模早期应用雌激素,可显示较好疗效,但ER-α饱和后,其疗效不随剂量增加而增强;在建模完成后应用雌激素,治疗效果欠佳,考虑是否与单一治疗有关,如能增加其他治疗方法也许能增强疗效。
[Abstract]:Aim: to study the effect of different doses of estradiol valerate (estradiol valerate,E2V) on intrauterine adhesion (intrauterine adhesions,IUA) in rats. Methods: 48 healthy female SD rats aged 8 weeks and 10 weeks were randomly divided into 8 groups: blank control group, experimental control group, three estrogen prevention groups (low, medium and high doses) and three estrogen treatment groups (low, medium and high doses). To observe the degree of endometrial fibrosis by HE staining in uterine tissue of rats in each group, and to observe the degree of endometrial fibrosis by MASson staining of endometrial gland. The expression of TGF- 尾 1 (transforming growth factor- 尾 1 TGF- 尾 1, matrix metalloproteinase-9 (matrix metalloproteinase-9,MMP-9) and estrogen receptor- 伪 (estrogen receptor- 伪 -ER- 伪) in endometrium were detected by immunohistochemical method. Results: compared with the control group, the endometrial gland count increased (P = 0.000) and the fibrosis area decreased (P _ (0.013) P _ (middle female A) in the three estrogen prevention groups (P _ (0.021) P _ (high female A) 0. 003). There were significant differences in the number of endometrial glands (P < 0.05) and the fibrosis area (P _ (0.013) P _ (middle female A) in the three estrogen prevention groups (P < 0.05). There was no significant difference in endometrial glandular count (P _ (low female B) 0.536P _ (middle female B) and fibrotic area (P = 1.000) between the control group and the control group. The expression level of TGF- 尾 1 in membrane was decreased (P = 0.000). The expression of MMP-9 in the high dose group was lower than that in the low dose group (P = 0.003 and 0.002, respectively), but there was no difference between the two groups (P < 0. 842), and the expression of MMP-9 in the endometrium of the six estrogen groups were all increased (P = 0.000), and those in the high dose group were higher than those in the low dose group (P = 0.000), while in the middle and high dose groups were higher than those in the low dose group (P = 0.000). There was no difference between the two groups (P < 0. 379), and the expression of ER- 伪 in the endometrium of the 6 estrogen groups was higher than that in the low dose group (P = 0.000), while the expression of ER- 伪 in the high dose group was higher than that in the low dose group (P = 0.000), but there was no difference between the middle and high dose groups (P < 0. 513). Conclusion: early use of estrogen in rat IUA model can show a good effect, but after ER- 伪 saturation, the therapeutic effect does not increase with the increase of dose, after modeling, the therapeutic effect of estrogen is not good enough to consider whether it is related to single therapy. If other treatments can be added, they may enhance the efficacy.
【作者单位】：南方医科大学珠江医院妇产科;
【基金】：国家自然科学基金资助项目(编号:81270658)
【分类号】：R711.74

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