尼古丁暴露孕鼠血代谢组学研究

发布时间：2018-09-10 21:08

【摘要】：目的研究尼古丁暴露孕鼠外周血代谢物改变特征,寻找可能反映胎鼠发育异常的早期特征性代谢物。方法将Wistar孕鼠16只分为空白对照组和尼古丁处理组,于孕9~20 d分别皮下注射生理盐水和尼古丁[2 mg/(kg·d)],观察孕鼠体重变化,并分析不同时间点(孕11、14、17、20 d)血浆1H NMR代谢谱改变。结果尼古丁处理后孕鼠体重增长减慢,孕20 d体重低于对照组(P0.01)。孕鼠血浆糖、脂和蛋白质代谢相关的多种物质发生改变,孕14 d为重要变化时间点。孕11~14 d血浆中柠檬酸、甘油、N-乙酰糖蛋白、缬氨酸、亮氨酸、色氨酸、赖氨酸、异亮氨酸、不饱和脂肪酸、低密度脂蛋白胆固醇、尿囊素浓度升高(P0.05),而丙酮酸、β-羟丁酸、肌醇浓度降低(P0.05)。结论 NMR代谢组学可作为孕鼠代谢改变检测的技术手段,β-羟丁酸、丙酮酸、柠檬酸、不饱和脂肪酸、甘油、缬氨酸、亮氨酸可能作为母体外周血中反映胎儿发育异常的早期特征代谢物。
[Abstract]:Objective to study the changes of metabolites in peripheral blood of pregnant rats exposed to nicotine and to search for the early characteristic metabolites which may reflect the abnormal development of fetal mice. Methods Sixteen Wistar pregnant rats were divided into blank control group and nicotine treated group. Normal saline and nicotine [2 mg/ (kg d)] were injected subcutaneously on the 20th day of gestation. The changes of body weight were observed and the changes of 1H NMR metabolic spectrum in plasma at different time points (11141720 days) were analyzed. Results the weight of pregnant rats decreased after nicotine treatment, and the body weight of pregnant rats on day 20 was lower than that of control group (P0.01). The plasma glucose, lipid and protein metabolism related substances were changed in pregnant rats, and 14 days of gestation was an important time point of change. Plasma citric acid, glycerol N-acetylglycoprotein, valine, leucine, tryptophan, lysine, isoleucine, unsaturated fatty acids, low density lipoprotein cholesterol, allantoin were elevated (P0.05), while pyruvate, 尾 -hydroxybutyric acid, 尾 -hydroxybutyric acid, pyruvate, 尾 -hydroxybutyric acid, 尾 -hydroxybutyric acid The concentration of inositol decreased (P0.05). Conclusion NMR metabolomics can be used as a technique to detect metabolic changes in pregnant rats, such as 尾 -hydroxybutyric acid, pyruvate, citric acid, unsaturated fatty acid, glycerol, valine. Leucine may be an early characteristic metabolite in maternal peripheral blood reflecting fetal dysplasia.
【作者单位】： 武汉大学基础医学院药理学系;
【基金】：国家自然科学基金(81430089);国家自然科学基金(81270950)
【分类号】：R714.5
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本文编号：2235618