Interceed对宫腔粘连兔子宫内膜容受性的影响

发布时间：2018-09-13 16:42

【摘要】：目的探讨氧化再生纤维素防粘连膜(Interceed)治疗新西兰大白兔宫腔粘连(IUA)的有效性及其对子宫内膜容受性的影响。方法将48只雌兔随机分为4组(每组12只):正常对照组(A)、Interceed对照组(B)、模型未治疗组(C)、Interceed治疗组(D)。A组行假手术,不造模;B组:正常兔子宫内放置Interceed;C和D组均采用机械和感染双重损伤法构建IUA动物模型,C组不进行治疗,D组建模后7d宫腔内放置Interceed。各组于28d各处死4只动物,收集双侧子宫组织,行HE和Masson染色检测子宫内膜腺体计数和纤维化面积。每组剩余的8只兔于动情期刺激阴道排卵,于假孕第7天处死,取子宫组织,扫描电镜观察子宫内膜胞饮突的表达,免疫组化检测子宫内膜中整合素αVβ3蛋白水平。结果与C组比较,D组子宫内膜腺体表达及纤维化程度均明显改善(P0.05),但A、B、D组两两比较差异均无统计学意义。A组子宫内膜表面有大量发育完全的胞饮突;B组胞饮突表达与A组相似;C组胞饮突分布稀少,发育不同步;D组胞饮突分布较C组增多,形态欠规则,发育同步。C组子宫内膜整合素αVβ3蛋白表达明显低于A组(P0.05);D组整合素αVβ3蛋白表达较C组明显增高(P0.05)。结论 Interceed可一定程度逆转IUA的病理改变,增加子宫内膜整合素αVβ3蛋白的表达,并改善胞饮突的表达和发育,从而改善子宫内膜的容受性。
[Abstract]:Objective to investigate the efficacy of oxidized regenerated cellulose antiadhesion membrane (Interceed) in the treatment of New Zealand rabbits with intrauterine adhesion (IUA) and its effect on endometrial receptivity. Methods 48 female rabbits were randomly divided into 4 groups (12 in each group): normal control group (A) interceed control group (B), model untreated group (C) interceed treatment group (D) .A group underwent sham-operation. Group B: normal rabbit intrauterine placement of Interceed;C and group D were used to construct IUA animal model by mechanical and infective double injury method. Group C did not undergo treatment and Interceed. was placed in uterine cavity 7 days after modeling in group D. Four animals died in each group on the 28th day. The bilateral uterine tissues were collected and the endometrial gland count and fibrosis area were detected by HE and Masson staining. The remaining 8 rabbits in each group stimulated vaginal ovulation during estrus and were killed on the 7th day of pseudopregnancy. The endometrial tissues were taken and the expression of endometrial cell processes was observed by scanning electron microscope. The level of integrin 伪 V 尾 3 protein in endometrium was detected by immunohistochemistry. Results compared with group C, the expression of endometrial glands and the degree of fibrosis in group D were significantly improved (P0.05), but there was no significant difference between group A and group B.There were a large number of fully developed cellular processes on the surface of endometrium in group A. Similar to group A, the distribution of the process was rare in group C. The expression of integrin 伪 V 尾 3 protein in group D was significantly lower than that in group A (P0.05), and the expression of integrin 伪 V 尾 3 protein in group D was significantly higher than that in group C (P0.05). Conclusion Interceed can reverse the pathological changes of IUA to some extent, increase the expression of integrin 伪 V 尾 3 protein, improve the expression and development of cellular processes, and improve the receptivity of endometrium.
【作者单位】：南方医科大学珠江医院妇产科;
【基金】：国家自然科学基金(81270658)~~
【分类号】：R711.74

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