稽留流产患者与正常早孕ADAM12、IGF-Ⅱ的对比研究

发布时间：2018-09-14 09:39

【摘要】：目的检测解整合素-金属蛋白酶12在血清、绒毛及蜕膜组织中的表达含量，，胰岛素样生长因子II在绒毛组织内的表达含量，旨在探讨ADAM12可作为判断早孕期胚胎发育水平的客观指标。 方法选择早孕期女性（5~10周）49例，其中正常妊娠26例（占53.1％），稽留流产23例（占46.9％）；留取血清，采用酶联免疫吸附实验法检测血清ADAM12水平；收集人工流产负压吸引术后绒毛组织及蜕膜组织，采用免疫组织化学染色方法，检测其ADAM12蛋白表达水平。收集人工流产负压吸引术后绒毛组织，采用免疫组织化学染色方法，检测其IGF-II表达水平。 结果正常早孕女性血清ADAM12水平随着孕周的增加而升高；在不同孕周，稽留流产组孕妇血清ADAM12含量均明显低于同孕周的正常妊娠孕妇，且差异具有统计学意义（p＜0.05）；稽留流产组绒毛组织内ADAM12表达量较少，且表达量低于正常妊娠组，差异具有统计学意义（p＜0.05）；在蜕膜组织内情况相同，且差异具有统计学意义（p＜0.05）；稽留流产组绒毛组织内IGF-II表达量较少，且表达量低于正常妊娠组，差异具有统计学意义（P＜0.05）；正常早孕女性绒毛组织内ADAM12与IGF-II表达量水平呈明显正相关关系（r=0.369，p=0.016）。 结论正常早孕血清ADAM12水平随着孕周的增加而升高；稽留流产组孕妇血清ADAM12水平明显降低；稽留流产组孕妇绒毛组织及蜕膜组织内ADAM12蛋白表达量均明显降低；稽留流产组孕妇绒毛组织内IGF-II表达量明显降低；稽留流产组孕妇ADAM12含量的减少可以推测ADAM12可作为判断早孕期胚胎发育水平的客观指标；ADAM12可以作为检测指标判断早孕结局；正常早孕女性绒毛组织内ADAM12与IGF-II表达量水平呈明显正相关关系，可以推测二
[Abstract]:Objective to detect the expression of integrin-metalloproteinase-12 in serum, villi and decidua, and the expression of insulin-like growth factor II in chorionic villi. The aim of this study was to explore that ADAM12 could be used as an objective index to judge the level of embryonic development in early pregnancy. Methods A total of 49 women (510 weeks) with early pregnancy were selected, of whom 26 were normal pregnancy (53.1%), 23 were missed abortion (46.9%), and the serum was collected and detected by enzyme linked immunosorbent assay (Elisa). The chorionic villi and decidua tissues of induced abortion were collected and the expression of ADAM12 protein was detected by immunohistochemical staining. The expression of IGF-II was detected by immunohistochemical staining. Results the serum ADAM12 level of normal early pregnant women increased with the increase of gestational weeks, and the serum ADAM12 levels of women with missed abortion were significantly lower than those of normal pregnant women of the same gestational age (p < 0. 05). The expression of ADAM12 in chorionic villi of missed abortion group was lower than that in normal pregnancy group (p < 0. 05), the same in decidua tissue and the difference was statistically significant (p < 0. 05). The expression of IGF-II in chorionic villi of missed abortion group was lower than that in normal pregnancy group (P < 0. 05), and the expression level of ADAM12 and IGF-II in chorionic villi of women with normal early pregnancy was positively correlated (r 0. 369 p0. 016). Conclusion the serum ADAM12 level of normal early pregnancy increased with the increase of gestational age, the serum ADAM12 level of missed abortion group decreased significantly, and the expression of ADAM12 protein in villi and decidua tissues of missed abortion group decreased significantly. The expression of IGF-II in chorionic villi of missed abortion group was significantly decreased, and the decrease of ADAM12 content in missed abortion group could be speculated that ADAM12 could be used as an objective index to judge the level of embryonic development in early pregnancy. ADAM12 could be used as an index to judge the outcome of early pregnancy. There was a significant positive correlation between the expression of ADAM12 and IGF-II in the chorionic villi of normal early pregnant women.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.2

【参考文献】

相关期刊论文 前10条

1 顾一村,高永利;隐匿性高催乳素血症与不孕症[J];国外医学.妇产科学分册;2002年02期

2 韦兴来;莫祝宁;晏湘梅;刘江泽;胡小韦;梁健;李坚;陈小玲;;经阴道彩色多普勒超声检测早期妊娠黄体的研究[J];广西医科大学学报;2008年05期

3 张锦虹;解脲支原体、人型支原体感染与胚胎停止发育的关系[J];昆明医学院学报;2001年04期

4 虞伟，秦雪，李晓军，武建国;抗心磷脂抗体与反复自然流产关系的探讨[J];临床检验杂志;1995年01期

5 张雪艳;顾依群;王金玲;刘晓红;王笑红;王军;闫蕾;赵一鸣;贾光;;北京市某区胚胎停育环境危险因素初步研究[J];环境与职业医学;2008年04期

6 赵永平;林典梁;张晓威;吴丹;沈浣;;精子细胞凋亡率与胚胎停育相关分析[J];中国计划生育学杂志;2012年09期

7 蔡春环;张霞;翟军;;孕妇衣原体、支原体感染及血清β-HCG、孕酮水平与胚胎停止发育的关系[J];山东医药;2011年30期

8 李晓艳;王泽华;;妊娠期维生素和微量元素的补充[J];实用妇产科杂志;2006年05期

9 林羿,洪彤,侯家珠;反复自然流产的几种免疫相关因素[J];生殖与避孕;1999年03期

10 李笑梅,曹淑莉,陈永华,汤明礼,王望九,王道屏,夏光惠,包继平;116例人工流产后不孕妇女抗精子和抗子宫内膜抗体分析[J];生殖与避孕;1999年04期

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