洛铂对体外培养人卵巢癌细胞增殖的抑制作用

发布时间：2018-10-08 18:12

【摘要】：目的观察洛铂(LBP)对体外培养人卵巢癌细胞增殖的抑制作用。方法选用7种人卵巢癌细胞株SK-OV-3、ES-2、OVCAR-8、3AO、SW626、A2780、OVCAR-3,以顺铂(DDP)、卡铂(CBP)为对照,评价LBP单药或联合紫杉醇(PTX)、多西他赛(TXT)对体外培养人卵巢癌细胞增殖的抑制作用及其联合用药效应。结果 LBP、DDP、CBP单药均可明显抑制体外培养人卵巢癌细胞增殖,IC50分别为1.5~13.8、0.9~8.1和14.6~145.7μmol·L-1。LBP、DDP、CBP联合PTX、TXT的效应具有细胞特异性,其中,LBP联合TXT在SK-OV-3、OVCAR-8细胞中显示相加效应。结论 LBP单药具有明确的体外抗人卵巢癌细胞增殖活性,其活性与DDP相当,强于CBP;LBP联合TXT对卵巢癌细胞有可能产生相加的抗肿瘤作用,但有细胞特异性。
[Abstract]:Objective to observe the inhibitory effect of roplatin (LBP) on the proliferation of human ovarian cancer cells in vitro. Methods Seven human ovarian cancer cell lines SK-OV-3,ES-2,OVCAR-8,3AO,SW626,A2780,OVCAR-3, were used to evaluate the inhibitory effect of LBP single drug or combination of paclitaxel (PTX), docetaxel (TXT) on the proliferation of human ovarian cancer cell line SK-OV-3,ES-2,OVCAR-8,3AO,SW626,A2780,OVCAR-3, in vitro and the combined effect of cisplatin (DDP), carboplatin (CBP). Results the inhibitory effects of LBP,DDP,CBP alone on the proliferation of human ovarian cancer cells in vitro were significantly inhibited as follows: IC50 was 1.5 ~ (13. 8) 渭 mol / L ~ (0.98.1) and 145.7 渭 mol / L ~ (-1) mol L ~ (-1), respectively. The additive effect of TXT combined with LBP,DDP,CBP was found in SK-OV-3,OVCAR-8 cells. Conclusion LBP has a distinct anti-proliferation activity in vitro, which is similar to that of DDP, and is stronger than that of CBP;LBP combined with TXT, which may produce additive anti-tumor effect on ovarian cancer cells, but it has cell specificity.
【作者单位】：贵州益佰制药股份有限公司医学部;溧阳市人民医院药剂科;中国科学院上海药物研究所;
【基金】：贵州省科技重大专项(黔科合中药字[2011]5068号)
【分类号】：R737.31

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