去氧肾上腺素介导的孕期母体高盐饮食对子代肾血管功能影响的研究

发布时间：2018-10-08 19:23

【摘要】：目的探讨母体高盐饮食对子代肾脏血管发育的影响及其相应机制。方法自然怀孕的Sprague-Dawley大鼠随机分为高盐组和对照组,高盐组孕鼠怀孕期间给予含8%NaCl的高盐饲料,对照组给予含1%NaCl的正常饲料,两组孕鼠均正常饮水。子代出生后,所有母鼠恢复正常饲料,新生鼠正常哺乳至1个月分笼。取成年子代公鼠肾脏叶间动脉进行血管功能检测、单个血管平滑肌(vascular smooth muscle cell,VSMC)细胞电生理实验。结果高盐组子代肾脏小动脉对去氧肾上腺素(phenylephrine,Phe)引起的收缩反应强于对照组(P0.05),蛋白激酶C(protein kinase C,PKC)非选择性阻断剂GF109203X对Phe收缩反应的作用两组间差异也有统计学意义(P0.05);电生理实验结果显示:Phe在两组子代肾脏小血管VSMC中,均可抑制大电导钙激活性钾通道(high-conductance Ca~(2+)-activated K+channel,BK通道)电流,但在高盐组的作用更为明显(P0.05),GF109203X的存在使BK电流在Phe作用前后差异无统计学意义(P0.05)。结论孕期高盐饮食可能通过改变Phe介导的成年♂性子代肾脏小动脉血管收缩的敏感性、PKC对BK通道的调控作用,从而影响其肾脏血管功能的发育,并可能由此增加其子代肾脏疾病发生的易感性。
[Abstract]:Objective to investigate the effect of maternal high salt diet on renal vascular development in offspring and its mechanism. Methods the naturally pregnant Sprague-Dawley rats were randomly divided into high salt group and control group. The pregnant rats in high salt group were given high salt diet containing 8%NaCl during pregnancy, while the control group were given normal diet containing 1%NaCl. After the birth of the offspring, all the female rats returned to normal feed, and the newborn mice suckled to 1 month cage. The vascular function of renal interlobar artery of adult male rat was measured and the electrophysiological experiment of single vascular smooth muscle (vascular smooth muscle cell,VSMC) cells was carried out. Results the contractile response of renal arterioles to noradrenaline (phenylephrine,Phe) in high salt group was stronger than that in control group (P0.05). The effect of GF109203X, a non-selective inhibitor of protein kinase C (protein kinase, on Phe contraction was significantly different between the two groups (P0.05). The results of electrophysiological experiments showed that in two groups of small renal vessel VSMC, The high-conductance Ca~ (2) -activated K channel,BK channel (high-conductance Ca~ (2) -activated K channel,BK channel) current was inhibited, but the effect was more obvious in high salt group (P0.05). The existence of GF109203X resulted in no significant difference in BK current before and after Phe treatment (P0.05). Conclusion High salt diet during pregnancy may affect the development of renal vascular function by altering the sensitivity of Phe mediated renal arterioles contraction and the regulation of BK channels. This may increase the susceptibility to kidney disease in their offspring.
【作者单位】：苏州大学附属第一医院生殖医学中心&胎儿医学研究所;
【基金】：国家自然科学基金资助项目(No 81370719) 江苏省社会发展项目(No BE2015642) 江苏省医学重点人才项目
【分类号】：R714

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