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激活的蛋白激酶C受体1 RACK1在宫颈上皮内瘤变及宫颈癌中的表达及意义

发布时间:2018-10-10 07:10
【摘要】:目的:1.检测激活的蛋白激酶C受体1(receptor for activated C kinase 1,RACK1)在正常宫颈、宫颈上皮内瘤变及宫颈鳞癌组织中的表达。2.结合高危型人乳头瘤病毒(high risk human papillomavirus,HR-HPV)检测结果,分析RACK1与HR-HPV感染的相关性。方法:随机选取于2015年10月-2016年10月在山西医科大学附属第二医院妇产科进行诊治的患者,其中行阴道镜活检、宫颈锥切术、子宫全切术、或宫颈癌手术后经病理证实为正常宫颈组织(正常对照)15例,早期宫颈鳞癌(cervical squamous cell carcinoma,CSCC)的标本15例,宫颈上皮内瘤变I级(Cervical intraepithe lia neoplasisa grade I,CINI)、宫颈上皮内瘤变II级(Cervical intraepithel ia neoplasisa grade II,CINII)、宫颈上皮内瘤变III级(Cervical intraepit helia neoplasisa grade III,CINIII)标本各30例。使用免疫组织化学SP法检测上述所有不同种类石蜡切片标本中RACK1表达情况,同时使用免疫印记western blot法分别检测RACK1在15例早期宫颈鳞癌及15例正常宫颈组织新鲜组织标本中的表达情况。同时取上述病人的宫颈分泌物标本采用HPV基因分型测定检测上述组织中HR-HPV感染情况,并对HR-HPV与RACK1的结果进行对照分析。从而探讨RAC K1蛋白在正常宫颈、宫颈上皮内瘤变及宫颈鳞癌组织中的表达与HR-HPV感染的相关性。结果:1.免疫组化法检测结果显示:RACK1蛋白在正常宫颈组织、CINI级组织,CINII级组织,CINIIII级组织和CSCC组织中的阳性表达率逐渐增高,依次为13.33%(2/15),30%(9/30),53.33%(16/30),80%(24/30)和100%(15/15),可见每组之间呈现逐渐升高的阳性表达率,五组间有明显的统计学差异(P0.05)。免疫印迹法检测结果显示:RACK1蛋白在宫颈鳞癌组和正常宫颈组中的相对表达水平分别为:0.89±0.07,0.29±0.01。宫颈鳞癌组与正常宫颈组阴性对照相比,RACK1的相对表达量增加了3.07倍,差异有统计学意义(P0.05)2.HR-HPV在正常宫颈组织、CINI级组织,CINII级组织,CINIIII级组织和CSCC组织中的阳性表达率分别为13.33%(2/15)、60%(18/30)、76.67%(23/30),90%(27/30)以及100%(15/15),四组间感染率的差异具有统计学意义(P0.05)。且经过Spearman相关性分析后显示,HP-HPV的感染同RACK1的阳性表达呈正相关性,相关系数r=0.266。结论:1.RACK1的阳性表达率均随着宫颈病变程度加重逐渐升高。且HR-HPV的阳性表达率均随着宫颈病变程度加重逐渐升高。2.RACK1的表达与HPV的感染呈正相关,表明RACK1蛋白可能在HPV致宫颈癌过程中起重要的作用。
[Abstract]:Purpose 1. The expression of activated protein kinase C receptor 1 (receptor for activated C kinase 1 rack1 was detected in normal cervix, cervical intraepithelial neoplasia and cervical squamous cell carcinoma. Combined with high risk human papillomavirus (high risk human papillomavirus,HR-HPV) detection results, the relationship between RACK1 and HR-HPV infection was analyzed. Methods: randomly selected patients from October 2015 to October 2016 in the second affiliated Hospital of Shanxi Medical University for gynecology and obstetrics, including colposcopy biopsy, cervical conization, and total hysterectomy. Or 15 cases of normal cervical tissue (normal control) and 15 cases of early cervical squamous cell carcinoma (cervical squamous cell carcinoma,CSCC) confirmed by pathology after operation of cervical carcinoma. There were 30 cases of cervical intraepithelial neoplasia (Cervical intraepithe lia neoplasisa grade I), 30 cases of cervical intraepithelial neoplasia (II grade (Cervical intraepithel ia neoplasisa grade II,CINII) and 30 cases of cervical intraepithelial neoplasia (III grade (Cervical intraepit helia neoplasisa grade III,CINIII). Immunohistochemical SP method was used to detect the expression of RACK1 in all kinds of paraffin sections. The expression of RACK1 in 15 cases of early squamous cell carcinoma of cervix and 15 cases of fresh tissue of normal cervix were detected by western blot. HPV genotyping was used to detect the infection of HR-HPV in the above tissues, and the results of HR-HPV and RACK1 were compared and analyzed. To investigate the expression of RAC K1 protein in normal cervix, cervical intraepithelial neoplasia and cervical squamous cell carcinoma (SCC) and its correlation with HR-HPV infection. The result is 1: 1. The results of immunohistochemical staining showed that the positive expression rate of 1% RACK1 protein in CINI grade III and CSCC tissues of normal cervix was increased gradually, which was 13.33% (2 / 15) 30% (9 / 30) and 80% (24 / 30) and 100% (15 / 15) respectively. There was significant statistical difference among the five groups (P0.05). The results of Western blotting showed that the relative expression level of 1% RACK1 protein in cervical squamous cell carcinoma group and normal cervical cancer group was 0.89 卤0.07 and 0.29 卤0.01, respectively. The relative expression of rack1 in cervical squamous cell carcinoma group was 3.07 times higher than that in normal cervical cancer group. The positive expression rates of 2.HR-HPV were 13.33% (2 / 15) and 60% (18 / 30) respectively in normal cervical tissue of CINI grade III and CSCC, respectively. The positive rates of 2.HR-HPV were 76.67% (23 / 30), 90% (27 / 30) and 100% (15 / 15), respectively. There was significant difference in the infection rate among the four groups (P0.05). Spearman correlation analysis showed that the infection of HP-HPV was positively correlated with the positive expression of RACK1, and the correlation coefficient was 0.266. Conclusion 1. The positive expression rate of RACK1 increased with the severity of cervical lesions. The positive expression rate of HR-HPV increased with the severity of cervical lesions. 2. The expression of RACK1 was positively correlated with the infection of HPV, indicating that RACK1 protein may play an important role in the process of cervical cancer induced by HPV.
【学位授予单位】:山西医科大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:R737.33

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