上皮性卵巢癌组织ATF4和RPL41表达及其临床意义

发布时间：2018-10-10 07:23

【摘要】：目的探讨转录激活因子4(activating transcription factor 4,ATF4)在许多肿瘤细胞的缺氧区被检测到呈高表达,并且与肿瘤的恶性程度相关。核糖体蛋白L41(ribosomal protein L41,RPL41)能够诱导肿瘤细胞死亡和细胞周期停滞,在调节ATF4的水平方面发挥着重要作用。本研究探讨ATF4和RPL41在上皮性卵巢癌组织中的表达及其与临床病理学特征的关系。方法利用免疫组化技术SP法分别检测中国医科大学附属盛京医院病理科2000-01-01-2015-12-31470例上皮性卵巢癌、42例交界性卵巢肿瘤、27例良性卵巢肿瘤和46例正常卵巢组织切片及芯片中ATF4和RPL41的表达情况。结果 ATF4在上皮性卵巢癌组织中阳性表达率为86.60%(407/470),明显高于正常卵巢与良性卵巢肿瘤组的39.73%(29/73)及交界性卵巢肿瘤组的73.81%(31/42),差异有统计学意义,P值分别为0.001和0.024。ATF4在上皮性卵巢癌组织中的表达明显高于其他组,并且与上皮性卵巢癌的组织病理学分级、临床分期、组织学类型及淋巴结转移均显著相关,P0.05。RPL41在正常卵巢与良性卵巢肿瘤组中的阳性表达率为82.19%(60/73),明显高于交界性卵巢肿瘤的54.76%(23/42)及上皮性卵巢癌的37.45%(176/470),差异有统计学意义,P值分别为0.002和0.001。RPL41在正常卵巢及卵巢良性肿瘤组织中的表达明显高于其他组;在上皮性卵巢癌中与淋巴结转移有关,P=0.016;但与组织病理学分级、临床分期及组织学类型均无关,P0.05。Spearman相关性分析显示,ATF4和RPL41在上皮性卵巢癌组织中呈中等程度相关,r=-0.508。结论 ATF4表达升高和RPL41表达降低可能在上皮性卵巢癌的发生、分化进展过程中起重要作用。
[Abstract]:Objective to investigate the high expression of transcriptional activator 4 (activating transcription factor 4 (ATF4) in the anoxic region of many tumor cells, which is related to the malignancy of the tumor. Ribosomal protein L41 (ribosomal protein L41 RPL41 can induce tumor cell death and cell cycle arrest, and play an important role in regulating the level of ATF4. This study was to investigate the expression of ATF4 and RPL41 in epithelial ovarian carcinoma and their relationship with clinicopathological features. Methods Immunohistochemical technique (SP) was used to detect histologic sections and microarrays in 42 cases of borderline ovarian tumors and 27 cases of benign ovarian tumors and 46 cases of normal ovarian tissues, respectively, from the Department of Pathology, Shengjing Hospital, affiliated to China Medical University, 2000-01-01-2015-31470 cases of epithelial ovarian carcinoma and 42 cases of borderline ovarian tumors. The expression of ATF4 and RPL41. Results the positive expression rate of ATF4 in epithelial ovarian cancer was 86.60% (407 / 470), which was significantly higher than that in normal and benign ovarian tumors (39.73%) and borderline ovarian tumors (73.81%) (31 / 42). The difference was statistically significant (P = 0.001 and 0.024.ATF4, respectively). The expression in ovarian cancer tissues was significantly higher than that in other groups. And with epithelial ovarian cancer histopathological grade, clinical stage, The positive expression rate of P0.05.RPL41 in normal and benign ovarian tumors was 82.19% (60 / 73), which was significantly higher than that in borderline ovarian tumors (54.76%, 23 / 42) and epithelial ovarian cancer (37.45%, 176 / 470) (P The expression of 0.001.RPL41 and 0.001.RPL41 in normal ovarian and ovarian benign tumors were significantly higher than those in other groups. In epithelial ovarian carcinoma, lymph node metastasis was associated with P0. 016, but it was not correlated with histopathological grade, clinical stage and histological type. The correlation analysis showed that ATF4 and RPL41 were moderately correlated in epithelial ovarian carcinoma tissues. Conclusion the increase of ATF4 expression and the decrease of RPL41 expression may play an important role in the development and progression of epithelial ovarian cancer.
【作者单位】：中国医科大学附属盛京医院妇产科;
【基金】：国家自然科学基金(81272873)
【分类号】：R737.31

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